Synthesis of Random DNA Libraries for In Vitro Selection and Analysis of Their Nucleotide Composition

Russian Journal of Bioorganic Chemistry - Aptamers are DNA or RNA molecules that specifically bind certain targets with high affinity due to the formation of unique spatial structures. Aptamers...     

Comparative evaluation of the efficiency of the effect of very high frequency electromagnetic waves on platelet functional activity

A comparative analysis was made of the effect of two kinds of EMI MMD-radiation: EMI MMD-waves, generated by a vehicle "Jav-1 M" (42.2 and 53.5 HHz), and EMI MMD-waves exerting influence with frequencies of molecular spectrum of radiation and nitric oxide absorption (150.176-150.644 HHz), obtained with a specially created generator, with respect to their influence on the functional ability of platelets of unstable angina pectoris patients. It was shown that in vitro EMI MMD-fluctuations with frequencies of molecular spectrum of radiation and nitric oxide absorption exert a stronger inhibiting influence on the functional activity of platelets of unstable angina pectoris patients. Features of the action of various kinds of EMI MMD-effect on the activative-high-speed characteristics of platelet aggregation are shown.     

Information EHF-interactions in a system of live objects (human platelets)

The molecular informational interaction has been first detected in a system that involves human platelets, exposed to electromagnetic EHF-fluctuations at frequencies of molecular spectra of radiation and absorption of nitric oxide (150.176-150.644 HHz), and native platelets. It has been established that the incubation of a native platelet rich plasma with a similar plasma, exposed to a 5-minute effect of electromagnetic EHF-fluctuations at frequencies of molecular spectra of radiation and absorption of nitric oxide at a mode of peak and frequent modulation of a signal under in vitro conditions, causes a significant (P < 0.05) inhibition of platelet functional activity in the native plasma, in comparison with control. This was displayed by a decreased platelet activation and falling platelet aggregation ability. Some possible mechanisms of interaction are suggested to explain the described effect.     

Electromagnetic radiation of the terahertz range at the nitric oxide frequency in correction and prophylaxis of functional activity disorders in thrombocytes of white rats under long-term stress

The influence of electromagnetic waves of terahertz range at the frequencies of molecular spectrum o nitric oxide radiation and absorption on functional activity of thrombocytes in white rats under long-term stress has been studied. It has been shown that courses of THzF treatment applied during the stress can prevent and restore disorders in thrombocytes aggregative function. The stress factor does not induce characteristic of stress-reaction disturbances of microcirculation in animals treated with the preventive course of THzF.     

Restoration of blood rheological properties by means of SWF-irradiation at the frequency of nitric oxide molecular spectrum (in vivo)

We have investigated impact of electromagnetic SWF-oscillations at nitric oxide molecular spectrum of radiation and absorption (MSRA) on rheological blood properties of albino rats under the immobilizing stress. The SWF-irradiation was demonstrated to foster restoration of disordered rheological blood properties, its efficiency depending on the period of irradiation.     

Influence of MHF-radiation on nitrogen oxide molecular spectrum frequency and coagulation factors under experimental stress-reactions

The effects of EMR MHF of molecular spectrum of radiation and absorption of nitrogen oxide on blood coagulation properties in white rats subjected to immobilization stress, have been studied. It has been revealed that MHF irradiation of the animals results in restoration of hemostasis mechanism indices.     

Effect of SWF-radiation on thrombocytes and erythrocyte functions of albino rats upon stress condition

With the help of a specially designed generator, we have investigated the effect of electromagnetic SWF-oscillation, at nitric oxide molecular spectrum of radiation and absorption, on the function of thrombocytes and erythrocytes of albino rats in the state of immobilizing stress. 5, 15 and 30 min long SWF-radiation treatments were demonstrated to foster various degrees of restoration of thrombocyte and erythrocyte function, the efficiency depending on the period of radiation. It was after a 30 min radiation of rats that a most expressed restoration of thrombocyte and erythrocyte functional activity was observed.     

Structural and functional organization of the plasmid regulons of <Emphasis Type="Italic">Rhizobium leguminosarum</Emphasis> symbiotic genes

The structure of the plasmid locus containing the sym-genes (nod-, nif-, and fix-operons) was investigated in eight Rhizobium leguminosarum strains differing in their origin and host specificity, including five strains of the viciae biovar—symbionts of pea (3), forage beans (1), and Vavilovia (1)—as well as three strains of the biovar trifolii (clover symbionts). Strains of R. leguminosarum bv. viciae, which possess the nodX gene (controlling acetylation of the Nod factor, which is responsible for the ability of rhizobia to form symbioses with a broad spectrum of hosts, including the “Afghan” pea lines, homozygous by the allele sym^2A), are characterized by a less compact location of the sym-genes than the strains lacking the nodX gene. The size of the symbiotic cluster in the strains possessing nodX was 94.5 ± 3.5 kb, with the share of the sym-genes of 36.5 ± 1.5%, while for the strains lacking nodX these values were 61.7 ± 3.7 kb and 56.3 ± 1.4%, respectively (significant difference at P ₀ < 0.01). Syntenic structures were revealed in the symbiotic regions of strains Vaf12, UPM1131, and TOM, as well as syntenic structures of non-symbiotic regions in strains Vaf12, TOM, and WSM1689. The correlation coefficients between the matrices of genetic distances in the analyzed strains for the nodABC, nifHDK, and fixABC operons were on average 0.993 ± 0.002, while their values for the plasmid sites located between the sym-genes were considerably less (0.706 ± 0.010). In these regions, 21 to 27% of the genes were involved in amino acid transport and metabolism, which was substantially higher than the average for the genome of R. leguminosarum bv. viciae (11–12%). These data suggest that the evolution of R. leguminosarum bv. viciae, defined by narrowing of the host specificity (associated with a loss of the nodX gene), was accompanied by reduction of the regions of plasmids located between the sym-genes, as well as by specialization of these areas to perform the functions related to symbiotic nitrogen fixation. The observed increase of density in the cluster of sym-genes may be associated with intensification of their horizontal transfer in the populations of rhizobia, which determines the speed of evolution of the symbiotic system.     

The effect of electromagnetic waves of very high frequency of molecular spectra of radiation and absorption of nitric oxide on the functional activity of platelets

A study was made of the effect of electromagnetic EMI MMD-fluctuation on the frequencies of molecular spectra of radiation, and nitric oxide absorption under in vitro conditions on the functional activity of platelets in patients with unstable angina pectoris, with the help of a specially created generator. At amplitude-modulated and continuous modes of EMI MMD-irradiation of platelet-rich plasma for 5, 15 and 30 min the platelet functional activity decreases, which was shown up in reduction of their activation and fall of aggregative ability. The degree, to which platelet functional activity was inhibited, depended on the mode of irradiation and on duration of EMI MMD effect. The most obvious changes in platelet activation and in their readiness to aggregative response were observed at a continuous mode of irradiation within a 15 min interval.