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Saina Josphat K. Li Zhi-Zhong Mekbib Yeshitila Gichira Andrew W. Liao Yi-Ying 《Molecular biology reports》2021,48(3):2007-2023
Molecular Biology Reports - Ailanthus altissima Swingle, is a tree species native to East Asia and has a great potential in decorative, bioenergy and industrial applications in many countries. To... 相似文献
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Mutant Thermotoga neapolitana DNA polymerase I: altered catalytic properties for non-templated nucleotide addition and incorporation of correct nucleotides
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Thermotoga neapolitana (Tne) DNA polymerase belongs to the DNA polymerase I (Pol I) family. The O-helix region of these polymerases is involved in dNTP binding and also plays a role in binding primer–template during DNA synthesis. Here we report that mutations in the O-helix region of Tne DNA polymerase (Arg722 to His, Tyr or Lys) almost completely abolished the enzyme’s ability to catalyze the template-independent addition of a single base at the 3′-end of newly synthesized DNA in vitro. The mutations did not significantly affect the DNA polymerase catalytic activity and reduced base misinsertions 5- to 50-fold. The same Arg722 mutations dramatically increased the ability of the enzyme’s 3′→5′ exonuclease to remove mispaired 3′ bases in a primer extension assay. These mutant DNA polymerases can be used to accurately amplify target DNA in vitro for gene cloning and genotyping analysis. 相似文献
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David Reber Mekbib Fekadu Florian Detsch Ralf Vogelsang Tamrat Bekele Thomas Nauss Georg Miehe 《Human ecology: an interdisciplinary journal》2018,46(4):587-600
This first survey of rock shelters and settlements in the Bale Mountains in Ethiopia is a baseline assessment for further research into the settlement history of Africa’s largest alpine highlands. Extensive GPS-based mapping and interviews resulted in two detailed maps, a catalogue of profiles, and complete photographic documentation. In total, 331 rock shelters (four permanently inhabited, 51 seasonally inhabited, and 276 currently uninhabited) and 870 settlements (207 permanently inhabited, 449 seasonally inhabited, 214 uninhabited) were recorded together with information about the activities and livelihoods of the inhabitants of the current settlements. This 2015 study was part of the Ethiopian-European research project “The mountain exile hypothesis – how humans benefited from and re-shaped African high-altitude ecosystems during Quaternary climate changes” (DFG FOR 2358). It was designed to support future management plans in this internationally important conservation area that has recently faced increasing land-use pressure and the threat of degradation. 相似文献
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Kflay Gebrehiwot Sebsebe Demissew Zerihun Woldu Mekbib Fekadu Temesgen Desalegn Ermias Teferi 《Plant Diversity》2019,(4):220-228
The aim of this research is to investigate the patterns of vascular plant species richness,diversity,and distribution along an elevation gradient in the Abune Yosef mountain range,Ethiopia.Preferential systematic sampling was employed to collect vegetation and environmental data along the elevation gradient.We found that plant species richness declines monotonically from low to high elevations.Specifically,vascular plant species richness and diversity were lower in the Afroalpine grassland(high elevation)than in the Dry evergreen Afromontane forest and Ericaceous forest(low elevations).In contrast,endemic vascular plant richness was significantly higher in the Afroalpine grassland than in the Dry evergreen Afromontane forest and Ericaceous forest.Elevation showed a significant impact on the richness,diversity,and endemism of vascular plants.According to Sorensen's coefficient,the similarity between Dry evergreen Afromontane forest and Ericaceous forest vegetation types is higher(32%)than the similarity between Ericaceous forest and Afroalpine grassland(18%).Only 5%similarity was recorded between the Dry evergreen Afromontane forest and Afroalpine grassland.Growth forms showed different elevationai richness patterns.Trees and liana increased monotonically up to 3300 m.Shrub and herb richness patterns followed a hump-shaped and inverted hump-shaped pattern along the elevation gradient.The elevation patterns of vascular plant species richness,diversity,and growth form in the present study may be attributed to differences in management intensity,spatial heterogeneity,microclimatic variations,and anthropogenic disturbances. 相似文献
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Mekbib Yeshitila Saina Josphat K. Tesfaye Kassahun Eshetu Girma Hu Guangwan Chen Jinming Li Zhizhong 《Plant Molecular Biology Reporter》2020,38(3):491-502
Plant Molecular Biology Reporter - Coffee is a popular beverage in the world and a valuable agricultural export commodity for several developing countries. Coffea arabica is one of the widely... 相似文献
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