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1.
Molecular Biology - Preeclampsia (PE) is a severe hypertensive pathology and affects 2–8% of pregnancies worldwide. Its etiopathogenesis is poorly understood, and prognostic biomarkers and... 相似文献
2.
Teppei Sugawara Ekaterina A. Trifonova Alex V. Kochetov Yoshinori Kanayama 《BMC plant biology》2016,16(3):246
Background
The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. However, many plant RNases are multifunctional and the direct link between their ribonucleolytic activity and antiviral defense still needs to be clarified. In this study, we evaluated the resistance of Nicotiana tabacum plants expressing a non-plant single-strand-specific extracellular RNase against Cucumber mosaic virus.Results
Severe mosaic symptoms and shrinkage were observed in the control non-transgenic plants 10 days after inoculation with Cucumber mosaic virus (CMV), whereas such disease symptoms were suppressed in the transgenic plants expressing the RNase gene. In a Western blot analysis, viral proliferation was observed in the uninoculated upper leaves of control plants, whereas virus levels were very low in those of transgenic plants. These results suggest that resistance against CMV was increased by the expression of the heterologous RNase gene.Conclusion
We have previously shown that tobacco plants expressing heterologous RNases are characterized by high resistance to Tobacco mosaic virus. In this study, we demonstrated that elevated levels of extracellular RNase activity resulted in increased resistance to a virus with a different genome organization and life cycle. Thus, we conclude that the pathogen-induced expression of plant apoplastic RNases may increase non-specific resistance against viruses with RNA genomes.3.
Shlyavas A. V. Trifonova A. A. Dedova L. V. Boris K. V. Kudryavtsev A. M. 《Russian Journal of Genetics》2019,55(11):1338-1346
Russian Journal of Genetics - The genetic diversity of 123 apple accessions from the collection of the research and production base Pushkin and Pavlovsk Laboratories of VIR, including landraces,... 相似文献
4.
Iu B Lishmanov Zh V Trifonova A N Tsibin L V Maslova L A Dement'eva 《Biulleten' eksperimental'no? biologii i meditsiny》1987,103(4):422-424
The experiments on white rats have shown that the induction of 4 hour stress produces an acute increase in beta-endorphin level, as well as characteristic changes in ACTH, cortisol, insulin, thyroxin and triiodothyronine concentrations. Different types of adaptation (training with short stress periods or injection of rhodiola rosea extract) promote a moderate increase in the amount of serum immunoreactive beta-endorphin, preventing its subsequent stress-induced elevation. Adaptation is characterized by a decrease or total prevention of hormonal changes peculiar to stress. The role of opioid neuropeptides in enhancing stress tolerance and the effect of adaptation factors are discussed. 相似文献
5.
Trifonova TV Maksiutova NN Timofeeva OA Chernov VM 《Prikladnaia biokhimiia i mikrobiologiia》2004,40(6):675-679
The activity of soluble lectins in leaves and roots of seedlings of winter wheat (Triticum aestivum L.) cultivar Mironovskaya 808 increased 1 day and 2 days, respectively, after infection with the mycoplasma Acholeplasma laidlawii 118. Analysis of acid-soluble proteins of wheat leaves by PAGE revealed the appearance of 22- and 20-kDa polypeptides, the disappearance of a 14-kDa polypeptide, and an increase in the content of polypeptides with molecular weights of 76, 48, 25, and 18 kDa. The 18-kDa polypeptide is a subunit of wheat germ agglutinin. A change in the activity of lectins may be a nonspecific response of plants to infection with the pathogen. 相似文献
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Singh BN Hayes GR Lucas JJ Sommer U Viseux N Mirgorodskaya E Trifonova RT Sassi RR Costello CE Fichorova RN 《Glycoconjugate journal》2009,26(1):3-17
Trichomonas vaginalis causes the most common non-viral sexually transmitted infection linked to increased risk of premature birth, cervical cancer
and HIV. This study defines molecular domains of the parasite surface glycoconjugate lipophosphoglycan (LPG) with distinct
functions in the host immunoinflammatory response. The ceramide phospho-inositol glycan core (CPI-GC) released by mild acid
had Mr of ∼8,700 Da determined by MALDI-TOF MS. Rha, GlcN, Gal and Xyl and small amounts of GalN and Glc were found in CPI-GC.
N-acetyllactosamine repeats were identified by endo-β-galactosidase treatment followed by MALDI-MS and MS/MS and capLC/ESI-MS/MS analyses. Mild acid hydrolysis led to products
rich in internal deoxyhexose residues. The CPI-GC induced chemokine production, NF-κB and extracellular signal-regulated kinase
(ERK)1/2 activation in human cervicovaginal epithelial cells, but neither the released saccharide components nor the lipid-devoid
LPG showed these activities. These results suggest a dominant role for CPI-GC in the pathogenic epithelial response to trichomoniasis. 相似文献
9.
Kolodiazgnaia IaS Titov SE Kochetov AV Trifonova EA Romanova AV Komarova ML Koval' VS Shumnyĭ VK 《Genetika》2007,43(7):994-998
Analysis of resistance of genetically modified tobacco plants bearing antisense suppressor of proline dehydrogenase gene and characterized with higher content of proline to elevated concentrations of heavy metals was performed. It was demonstrated that progeny of transgenic plants have high resistance to lead, nickel and cadmium ions. 相似文献
10.
Kochetov AV Titov SE Kolodiazhnaia IaS Komarova M Koval' VS Makarova NN Ilinskiĭ IuIu Trifonova EA Shumnyĭ VK 《Genetika》2004,40(2):282-285
The antisense suppressor was constructed for proline dehydrogenase gene (PDH; a fragment of PDH from Arabidopsis in antisense orientation and under the control of 35S promoter of cauliflower mosaic virus, CMV). In Nicotiana tabacum SR1 tobacco transformants bearing antisense suppressor for PDH, the proline content and the cytoplasm osmotic pressure were increased. The proline content in these transformants varied, whereas cytoplasm osmotic pressure was stable, which seems to reflect complicated relationships between these characteristics of the plant cell. 相似文献