排序方式: 共有23条查询结果,搜索用时 140 毫秒
1.
Kovalev LI Shishkin SS Khasigov PZ Dzeranov NK Kazachenko AV Toropygin IIu Mamykina SV 《Prikladnaia biokhimiia i mikrobiologiia》2006,42(4):480-484
A comparative analysis of the proteins in prostate tissues of the patients operated for hyperplasia (n = 7) or cancer (n = 5) was performed aiming to search for protein diagnostic markers. Differences in several minor proteins were detected using two-dimensional electrophoresis according to O'Farrel; among them, an additional protein with a molecular weight of 19 kDa and an isoelectric point of 9.0 was observed in four of the cancer cases. Mass spectrometry allowed this protein to be identified as the androgen-induced secreted protein AGR2. The possibility of using AGR2 as a diagnostic marker of prostate cancer is discussed. 相似文献
2.
Alla M. Andreeva Nina Lamash V. I. Martemyanov A. S. Vasiliev I. Yu. Toropygin D. V. Garina 《Journal of fish biology》2024,104(3):564-575
To investigate the stress response and physiological adaptations of goldfish (Carassius auratus) to critical salinity (CS) waters, we analyzed high-density lipoprotein (HDL) stoichiometry, stress markers (cortisol, glucose), and plasma osmotic properties (Na+, osmolality, water content) using ichthyology, biochemistry, and proteomics approaches. After 21 days of exposure to CS, plasma concentrations of cortisol, glucose, and Na+ increased, indicating stress. Total plasma osmolality (Osmtotal) and osmolality generated by inorganic (Osminorg) and organic osmolytes (Osmorg) also increased, the latter by ~2%. We associated the increase of Osmorg with (1) increased metabolite concentration (glucose), (2) dissociation of HDL particles resulting in increased HDL number per unit plasma volume (~1.5–2-fold) and (3) increased HDL osmotic activity. HDL remodeling may be the reason for the redistribution of bound and free water in plasma, which may contribute to water retention in plasma and, at the same time, to hemodynamic disturbances under CS conditions. The study's findings suggest that HDL remodeling is an important mechanism for maintaining osmotic homeostasis in fish, which is consistent with current capillary exchange models in vertebrates. 相似文献
3.
Nikita Biziaev Elizaveta Sokolova Dmitry V. Yanvarev Ilya Yu Toropygin Alexey Shuvalov Tatiana Egorova Elena Alkalaeva 《The Journal of biological chemistry》2022,298(7)
The nucleotide context surrounding stop codons significantly affects the efficiency of translation termination. In eukaryotes, various 3′ contexts that are unfavorable for translation termination have been described; however, the exact molecular mechanism that mediates their effects remains unknown. In this study, we used a reconstituted mammalian translation system to examine the efficiency of stop codons in different contexts, including several previously described weak 3′ stop codon contexts. We developed an approach to estimate the level of stop codon readthrough in the absence of eukaryotic release factors (eRFs). In this system, the stop codon is recognized by the suppressor or near-cognate tRNAs. We observed that in the absence of eRFs, readthrough occurs in a 3′ nucleotide context-dependent manner, and the main factors determining readthrough efficiency were the type of stop codon and the sequence of the 3′ nucleotides. Moreover, the efficiency of translation termination in weak 3′ contexts was almost equal to that in the tested standard context. Therefore, the ability of eRFs to recognize stop codons and induce peptide release is not affected by mRNA context. We propose that ribosomes or other participants of the elongation cycle can independently recognize certain contexts and increase the readthrough of stop codons. Thus, the efficiency of translation termination is regulated by the 3′ nucleotide context following the stop codon and depends on the concentrations of eRFs and suppressor/near-cognate tRNAs. 相似文献
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5.
Alexander T. Eprintsev Marina I. Falaleeva Maya S. Lyashchenko Ilya Y. Toropygin 《Bioscience, biotechnology, and biochemistry》2018,82(1):81-89
Malate dehydrogenase (EC 1.1.1.37) was purified to homogeneity from the phototrophic purple non-sulfur bacterium Rhodovulum steppense A-20s. According to gel-chromatography and electrophoretic studies, malate dehydrogenase is present as a dimer, tetramer and octamer depending on cultivation conditions. In phototrophic aerobic conditions only the tetrameric form was present, in chemotrophic aerobic conditions all three forms were detected, while in the absence of oxygen the octameric form disappeared. The malate dehydrogenase oligomers are encoded by a single gene and composed of the same 35 kDa polypeptide but differ in pH and temperature optimum, in affinities to malate, oxaloacetate, NADH and NAD+ and in regulation by cations and citrate. By modulating the cultivation conditions, it has been established that the dimer participates in the glyoxylate cycle; the tetramer operates in the tricarboxylic acid cycle, and the octamer may be involved in the adaptation to oxidative stress. 相似文献
6.
L. I. Vorob’eva E. Yu. Khodzhaev A. L. Mulyukin I. Yu. Toropygin 《Applied Biochemistry and Microbiology》2009,45(5):489-493
Reactivating factor (RF) from Luteococcus japonicus subsp. casei was shown to be constitutively synthesized and to act a by one-step mechanism, being activated independently from stress.
Cell reactivation (reversion of a cell’s ability to form macrocolonies) might be ensured by the membrane mechanism of RF action,
which is proved with the dependence of antistress activity from the condition of the cytoplasmic membrane and with the form
of concentration dependence. The incubation of UV-treated L. casei suspension with RF increased the number of cells with intact barrier membrane (1.6–1.8-fold increase compared to RF-untreated
cells) and the number of colony-forming cells. Cross defensive and reactivating RF effects on both L. casei and yeast Saccharomyces cerevisiae cells were described. Bacterial and yeast’s RF compete for membrane receptors. Matrix Assisted Laser Desorption/Ionization
time-of-flight (MALDI-TOF) spectrometry revealed that RF of L. casei contained two major peptides of 5.8 and 7.6 kDa, while RF of S. cerevisiae was represented by a single peptide of 5.8 kDa. The presence of 5.8 kDa peptide in RF from bacteria and yeasts might ensure
cross responses in these organisms. 相似文献
7.
L. I. Kovalyov S. S. Shishkin P. Z. Khasigov N. K. Dzeranov A. V. Kazachenko M. A. Kovalyova I. Yu. Toropygin S. V. Mamykina 《Applied Biochemistry and Microbiology》2006,42(4):424-427
A comparative analysis of the proteins in prostate tissues of the patients operated for hyperplasia (n = 7) or cancer (n = 5) was performed aiming to search for protein diagnostic markers. Differences in several minor proteins were detected using two-dimensional electrophoresis according to O’Farrel; among them, an additional protein with a molecular weight of 19 kDa and an isoelectric point of 9.0 was observed in four of the cancer cases. Mass spectrometry allowed this protein to be identified as the androgen-induced secreted protein AGR2. The possibility of using AGR2 as a diagnostic marker of prostate cancer is discussed. 相似文献
8.
E. O. Fedina F. G. Karimova I. A. Tarchevsky I. Yu. Toropygin V. A. Khripach 《Russian Journal of Plant Physiology》2008,55(2):193-200
Two-dimensional electrophoresis was used to separate proteins from crude extracts of pea (Pisum sativum L.) leaves, and thus isolated proteins were subjected to Western blot analysis with monoclonal antibodies against PY20 phosphotyrosine polypeptides. This analysis revealed 44 polypeptides phosphorylated on tyrosine residues. Phosphorylation of some of these proteins was changed under the action of epibrassinolide. Some of these polypeptides were identified by means of MALDI-TOF MS analysis. The results indicate that eight of these proteins belong to the Calvin cycle enzymes, namely, the isoforms of Rubisco large and small subunits, fructose-1,6-phosphate aldolases 1 and 2, and the precursor of α-subunit of Rubisco-binding protein. The observed changes in phosphorylation of these proteins may partly explain the effects of brassinosteroids on photosynthesis. The tyrosine phosphorylation sites were identified in silico for the fragments of polypeptides examined. 相似文献
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10.
Mirgorodskaya O. A. Kozmin Y. P. Protasov A. D. Toropygin I. Y. Oleinikov V. A. 《Russian Journal of Bioorganic Chemistry》2018,44(6):665-675
Russian Journal of Bioorganic Chemistry - The potential of the nitro compounds of the azoloazine class as regulators of aggregation of natural self-associating peptides was demonstrated by the... 相似文献