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排序方式: 共有56条查询结果,搜索用时 15 毫秒
1.
Erythrocytes suspended in a medium of low ionic strength lyse under the effect of an exponential electrical pulse. The percentage of haemolysed cells decreases several-fold in the presence of divalent cations. The protective action of the ions studied increases in the following order: Ca++, Mg++, Zn++. It is assumed that divalent ions bind to the negative charges of the lipid and protein molecules and reduce their electrostatic repulsion, which results in stabilization of the membranes. 相似文献
2.
N Tsanev E Tsvetkova A Tomov 《Journal of hygiene, epidemiology, microbiology, and immunology》1984,28(3):303-308
The applicability of coagglutination for the rapid detection and serogrouping of Legionellae has been investigated. The coagglutination reaction is carried out with the aid of self-made preparations of protein A containing staphylococci, sensitized with specific antibodies against the antigens of L. pneumophila (serogroups I to 6), L. bozemanii and L. micdadei. Preliminary heating of Legionella suspensions at 100% C for 15 min was used to prevent cross coagglutination reactions and ensure greater safety of laboratory personnel during the performance of the test. The results obtained demonstrate a high specificity of coagglutination. With the aid of the coagglutination reactions it has been shown that L. pneumophila strains isolated in Bulgaria belong to serogroup I. The coagglutination method is characterized by its rapidity, simplicity and feasibility. It is a useful and convenient means for the rapid detection and serogrouping of Legionellae. 相似文献
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4.
The effect of neurotensin on submaximally-stimulated hepatobiliary and pancreatic secretion was studied in 6 healthy subjects. An intravenous infusion of neurotensin 1.4 ± 0.3 pmol/kg/min, designed to reproduce plasma neurotensin immunoreactivity levels within the physiological range, produced a significant increase in pancreatic bicarbonate output. Plasma concentrations of pancreatic polypeptide rose by 83 ± 16 pmol/l and were associated with a small reduction in trypsin, but no significant change in bilirubin outputs. 相似文献
5.
We isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross‐species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species). 相似文献
6.
Parallorhogas pyralophagus (Marsh) is the principal parasitoid of the Mexican rice borer [Eoreuma loftini (Dyar)], the primary pest of sugarcane in south Texas. Insect resistant transgenic sugarcane producing Galanthus nivalis agglutinin (GNA) was developed to improve control of E. loftini. The present laboratory study addressed whether over two consecutive generations transgenic sugarcane delivered via artificial diet has adverse host-mediated effects on P. pyralophagus life history parameters. The results of this study showed that a number of life history parameters were affected by transgenic sugarcane, whereas others were not affected, and that effects varied between generations. In the first generation, adult longevity was increased by approximately 2 d, and cocoon to adult and egg to adult developmental times were prolonged by approximately 1 d in parasitoids exposed to transgenic sugarcane, whereas effects were not evident on adult size; egg load; egg to cocoon developmental time; rates of gain of longevity and egg load with adult size; and egg, larval, and pupal mortality. However, in the second generation, adult longevity was reduced by approximately 3 d, adult size by approximately 5%, egg load by approximately 24%, and rate of gain of longevity with adult size by approximately 21%, whereas effects were not evident on the rate of gain of egg load with adult size. It was concluded that although GNA transgenic sugarcane, ingested via E. loftini tissues, was not acutely toxic to P. pyralophagus, the sublethal effects on life history parameters measured in this study must be considered in a broader context to determine their possible ecological significance. 相似文献
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Long runs of seasonal rotifer population data allow analysis of seasonal occurrence using mathematical tools. The application of Fourier analysis to a 15 year dataset describes seasonality in simple mathematical terms. This facilitates comparison of population expression with potential population driving variables and provides a basic modelling tool. Results show that annual patterns of occurrence and density have linkages with annual maximum and minimum environmental temperature, although the exact relationships are not clear. 相似文献
9.
Induction of apoptosis by electrotransfer of positively charged proteins as Cytochrome C and Histone H1 into cells 总被引:1,自引:0,他引:1
Tsoneva I Nikolova B Georgieva M Guenova M Tomov T Rols MP Berger MR 《Biochimica et biophysica acta》2005,1721(1-3):55-64
Cytochrome C (Cyt. C) is a mitochondrial protein inducing apoptosis when it is accumulated in the cytosol by a currently unknown mechanism, but regulated by the bcl-2 family of proteins. The linker Histone H1 is another basic protein with highly conservative structure, composition, and equal molecular weight, not changed during the evolution. An attempt was made to understand better the apoptotic processes by electroloading of leukemic cells, such as K562, HL-60, and SKW3, and human lymphocytes with positively charged proteins, such as Cyt. C, Histone H1, and methylated BSA albumin (mBSA). The triggering apoptotic processes followed by MTT test, FACS analysis, and DNA fragmentation after the electrotransfer of these proteins into the cells were observed. Histone H1 and mBSA induce the release of Cyt. C from rat liver mitochondria. Cytochrome C release was higher when mitochondria were in "high-energy" state. It is supposed that release of Cyt. C from mitochondria is due to the mechanical rupture of the outer mitochondrial membrane, rich in negatively charged groups, predominately due to cardiolipin. The reason for the morphological rupture of the outer mitochondial membrane could be the rigidification and segregation of the membrane and the destroyed membrane asymmetries of both monolayers in the presence of positively charged proteins at higher linear charges such as Histone H1. We suggested that Histone H1, at a given moment of activated signaling for apoptosis, could be not transported to the nucleus and could lead to the release of Cyt. C from the mitochondria in the cytoplasm. It is temping to speculate that Histone H1 has other physiological extranuclear functions involved in apoptosis. 相似文献
10.
Ziver Sahin Muge Bıcakcıgil Kenan Aksu Sevil Kamali Servet Akar Fatos Onen Omer Karadag Zeynep Ozbalkan Askin Ates Huseyin TE Ozer Vuslat Yilmaz Emire Seyahi Mehmet A Ozturk Ayse Cefle Veli Cobankara A Mesut Onat Ercan Tunc Nursen Düzgün Sibel Z Aydin Neslihan Yilmaz İzzet Fresko Yasar Karaaslan Sedat Kiraz Nurullah Akkoc Murat Inanc Gokhan Keser F Aytul Uyar Haner Direskeneli Güher Saruhan-Direskeneli 《Arthritis research & therapy》2012,14(1):R27-5