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排序方式: 共有433条查询结果,搜索用时 31 毫秒
1.
Endothelin, the most potent vasoconstrictor found in nature, is thought to be important in the regulation of blood pressure and/or local blood distribution. Human placenta cDNA fragment encoding preproendothelin-1 (preproET-1) and its carboxyl terminal mature precursor (C-matured precursor) was expressed in E. coli. These products were characterized by both enzyme immunoassay and Western blot analysis. 相似文献
2.
K Tomoo H Ohishi M Doi T Ishida M Inoue K Ikeda Y Hata Y Samejima 《Biochemical and biophysical research communications》1992,184(1):137-143
The crystal structure of acidic phospholipase A2 from the venom of Agkistrodon halys blomhoffii has been determined by molecular replacement methods based on the known structure of Crotalus atrox PLA2, a same group II enzyme. The overall structures, except the calcium-binding regions, are very similar to each other. A calcium ion is pentagonally ligated to two carboxylate oxygen atoms of Asp-49 and each carbonyl oxygen atoms of Tyr-28, Gly-30 and Ala-31. A reason why the former enzyme functions as monomeric form, while the latter one does as dimer, could be presumed by the structural comparison of these calcium-binding regions. Although Gly-32 is usually participated as a ligand in the coordination with calcium ion in group I PLA2, it is characteristically replaced to Ala-31 in the present structure, and thus the coordination geometry of calcium ion is rather different from the usually observed one. 相似文献
3.
Seasonal changes in the spermatogenic epithelium of adult Japanese macaques (Macaca fuscata fuscata)
Tomoo Enomoto Kiyoaki Matsubayashi Yasukazu Nagato Mayumi Nakano 《Primates; journal of primatology》1994,35(4):465-472
A histological study was undertaken to clarify seasonal changes in the spermatogenic epithelium of Japanese macaques. Testicular
tissue samples were excised by biopsies from five adult laboratory-maintained males in mating and non-mating seasons. The
samples were fixed with Bouin's solution, embedded in paraffin, and stained with PAS and hematoxylin. Microscopic observations
on cross-sections of seminiferous tubules revealed that the seminiferous epithelium in the mating season was thicker than
in the non-mating season. PAS-stained granules were found in some of the dark A-type spermatogonia, which significantly increased
in the non-mating season. Spermatids of the steps preceding the appearance of the acrosomic cap in stages I to III were observed
significantly more often than those in the step coinciding with the formation of the acrosomic cap in stage IV. In stage I,
the ratio of mature spermatids or spermatozoa to immature spermatids in the mating season was higher than that in the non-mating
season. These findings suggest that spermiogenesis, as well as spermatocytogenesis, is inhibited in the non-mating season. 相似文献
4.
Hideaki Matsuoka Yasushi Kazuno Takuji Horie Tomoo Homma Yasuyuki Nemoto 《Cytotechnology》1993,11(1):59-65
Possible roles of coexisting cells in inducing neurite growth from a nerve cell were studied. Nerve growth factor (NGF)-inducing neurite growth from PC12h-R (a cell line derived from cultured nerve cells) was investigated at various cell densities. At the cell density 102104 cells/ml neurites appeared even without NGF. In contrast, no neurite appeared without NGF in single cell culture. The neurite growth observed in plural cell culture without NGF was only partially inhibited by antibody to NGF receptor (Ab-NGFR). However, the effect of the used medium alone was mostly inhibited by Ab-NGFR. These results suggest that the neurite inducing potency of coexisting cells is via different sites than the NGF receptor.Abbreviations Ab-IgG-FITC
anti-mouse-IgG labeled with fluorescein isothiocyanate
- Ab-NF
monoclonal antibody to neurofilament 160 kD
- Ab-NGFR
monoclonal antibody to NGF receptor
- BDNF
brain-derived neurotrophic factor
- D-medium
medium for differentiation culture
- DMEM
Dulbecco's modified Eagle's medium
- M-medium
medium for multiplication culture
- NGF
nerve growth factor
- NGFR
NGF receptor
- NT-3
neurotrophin-3
- PC12
pheochromocytoma cell line
- PC12h-R
subclone of PC12
- Sup-D
supernatant of D-medium 相似文献
5.
6.
A Yamamoto T Kaji K Tomoo T Ishida M Inoue M Murata K Kitamura 《Journal of molecular biology》1992,227(3):942-944
Cathepsin B from bovine spleen has been purified and crystallized as a complex with a specific inhibitor CA074 [N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-L- isoleucyl-L-proline], using the hanging-drop method. The complex crystals obtained from 50 mM-citrate buffer (pH 3.5) belong to the tetragonal space group P4(1) (or P4(3)) with a = 73.06 A and c = 141.59 A, and diffract beyond 2.2 A resolution. There are two complex molecules per asymmetric unit giving a packing density of 3.37 A3/Da and indicating a high solvent content of 63.5%. 相似文献
7.
8.
Shohei Yamaoka Yuki Shimono Makoto Shirakawa Yoichiro Fukao Takashi Kawase Noriyuki Hatsugai Kentaro Tamura Tomoo Shimada Ikuko Hara-Nishimura 《The Plant cell》2013,25(8):2958-2969
The adaptor protein-2 (AP-2) complex is a heterotetramer involved in clathrin-mediated endocytosis of cargo proteins from the plasma membrane in animal cells. The homologous genes of AP-2 subunits are present in the genomes of plants; however, their identities and roles in endocytic pathways are not clearly defined in plants. Here, we reveal the molecular composition of the AP-2 complex of Arabidopsis thaliana and its dynamics on the plasma membrane. We identified all of the α-, β-, σ-, and μ-subunits of the AP-2 complex and detected a weak interaction of the AP-2 complex with clathrin heavy chain. The μ-subunit protein fused to green fluorescent protein (AP2M-GFP) was localized to the plasma membrane and to the cytoplasm. Live-cell imaging using a variable-angle epifluorescence microscope revealed that AP2M-GFP transiently forms punctate structures on the plasma membrane. Homozygous ap2m mutant plants exhibited abnormal floral structures, including reduced stamen elongation and delayed anther dehiscence, which led to a failure of pollination and a subsequent reduction of fertility. Our study provides a molecular basis for understanding AP-2–dependent endocytic pathways in plants and their roles in floral organ development and plant reproduction. 相似文献
9.
Masanori Tachikawa Kazunari Suzuki Kaoru Iguchi Tomoo Miyazaki 《Molecular simulation》2013,39(3-6):291-298
Abstract The weak interaction energy of H2 dimer is studied by double symmetry-adapted perturbation theory (SAPT) within second-order of intermolecular and intramonomer perturbation for molecular simulations. The assumed orientations of H2 dimer are linear, parallel, T type and X type. Among four orientations T orientation is the most stable, while linear orientation is the most repulsive. The second-order dispersion energy E disp (2) is the most attractive contribution in all orientations. The interaction energy has the anisotropy, so we expressed our total interaction energy by the spherical expansion to compare with the experimental value. The isotropic interaction energy is about 85% of the experimental value. 相似文献
10.