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1.
E A Gubina M M Zheludkov S I D'iakov I K Lebedeva T A Tolmacheva 《Antibiotiki i khimioterapii͡a》1989,34(2):109-112
Optimal conditions for rapid assay of Brucella antibiotic sensitivity with the immunofluorescent method were developed. With this method high sensitivity of the main Brucella species to tetracycline, doxycycline and rifampicin was confirmed. It was found actually possible to use the immunofluorescent method for rapid assay of Brucella antibiotic sensitivity in practice. 相似文献
2.
AV Shevchenko IG Budzanivska TP Shevchenko VP Polischuk D Spaar 《Archives Of Phytopathology And Plant Protection》2013,46(2):139-146
The work was focused on the investigation of possible dependencies between the development of viral infection in plants and the presence of high heavy metal concentrations in soil. Field experiments have been conducted in order to study the development of systemic tobacco mosaic virus (TMV) infection in Lycopersicon esculentum L. cv. Miliana plants under effect of separate salts of heavy metals Cu, Zn and Pb deposited in soil. As it is shown, simultaneous effect of viral infection and heavy metals in tenfold maximum permissible concentration leads to decrease of total chlorophyll content in experiment plants mainly due to the degradation of chlorophyll a. The reduction of chlorophyll concentration under the combined influence of both stress factors was more serious comparing to the separate effect of every single factor. Plants' treatment with toxic concentrations of lead and zinc leaded to slight delay in the development of systemic TMV infection together with more than twofold increase of virus content in plants that may be an evidence of synergism between these heavy metal's and virus' effects. Contrary, copper although decreased total chlorophyll content but showed protective properties and significantly reduced amount of virus in plants. 相似文献
3.
T. Yu. Tolmacheva 《Paleontological Journal》2013,47(2):185-189
Naimanodus degtyarevi gen. et sp. nov., a new conodont genus and species from the Lower Darriwilian (Middle Ordovician) limestones of the Naiman Formation in central Kazakhstan is described. Three types of coniform nongeniculate elements are identified in the apparatus of this species. The new species was widespread in the Asian Paleobasin and recorded in three localities in Kazakhstan, eastern Kyrgyzstan, and Altai. 相似文献
4.
Chepurnov SA Chepurnova NE Tolmacheva EA Kochetkov IaA van Luijtelaar EL Coenen AM 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2002,88(6):741-750
Progesterone and oestradiol serum level was investigated in WAG/Rij rats with genetically determined absences. Blood samples were drawn before and after the pregnancy following the parturition. The serum concentration of progesterone increased after the 3rd day of pregnancy. There is no increasing of oestradiol during pregnancy as large as this. The progesterone is kept high to the 18th day of pregnancy and drastically decreased before the parturition. Common duration of absences--spontaneous spikewave discharges (SWD), frequency and the duration of every SWD decreased from 3rd to 19th days of pregnancy before the parturition. On the basis of these data and modern investigations, regulation of GABAA receptor expression during pregnancy by progesterone (Brusaartd A. B. et al., 1999) it can be assumed that the changes in the parameters of SWD are possibly correlated with the progesterone changes in serum during pregnancy in WAG/Rij rats. 相似文献
5.
6.
N. V. Galayko I. A. Tolmacheva V. V. Grishko L. V. Volkova E. N. Perevozchikova S. A. Pestereva 《Russian Journal of Bioorganic Chemistry》2010,36(4):516-521
Novel hydrazones of the lupane and 19β,28-epoxy-18α-oleanane types have been synthesized via the interaction of 2,3-secotriterpenic
aldehydonitriles with substituted hydrazines. As a result of the investigation of the antiviral activity of 2,3-secotriterpenic
hydrazones against the Indiana strain of the vesicular stomatitis virus on two models of mammalian cell line infection, the
acetylhydrazone of 1-cyano-2,3-seco-19β,28-epoxy-18α-olean-3-al has been found to have a high prophylactic activity of 0.00016
μg/ml to the vesicular stomatitis virus and to inhibit virus reproduction in primarily infected cells in a 0.21-μg/ml concentration. 相似文献
7.
Fabrizio Grandi Marcia M Colodel Lidianne N Monteiro João Rafael VP Leão Noeme S Rocha 《BMC veterinary research》2010,6(1):45
Backgroud
Extramedullary hematopoiesis (EMH) is defined as the presence of hematopoietic stem cells such as erythroid and myeloid lineage plus megakaryocytes in extramedullary sites like liver, spleen and lymph nodes and is usually associated with either bone marrow or hematological disorders. Mammary EMH is a rare condition either in human and veterinary medicine and can be associated with benign mixed mammary tumors, similarly to that described in this case. 相似文献8.
Yu. P. Tolmacheva 《Journal of Ichthyology》2010,50(1):65-72
The structure of the mouth opening and pharyngeal apparatus was studied, and its relation to the feeding pattern in three
coastal species of Baikal Cottidae fish was analyzed. Analysis of data demonstrated that the general pattern of structure
of the mouth opening and pharyngeal apparatus is similar in the species under study since their food is represented by bottom
organisms, mainly by amphipods. Interspecies differences are manifested in an increase in relative values of morphological
features and the size of consumed prey. 相似文献
9.
Highly sensitive enzyme-linked assay based on monoclonal antibodies for detection of Brucella antigens 总被引:1,自引:0,他引:1
Fel'dsherova AA El'gort DA Konopleva MV Khats IuS Tret'iakov OIu Kulakov IuK Tolmacheva TA Zheludkov MM Suslov AP 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2007,(1):52-57
Mice monoclonal antibodies against lypopolysaccharides (LPS) of Brucella abortus has been obtained and characterized. The antibodies detected LPS of B. abortus, B. melitensis and B. suis with high sensivity and specificity and did not react with LPS of Yersinia enterocolitica O:3, Y. enterocolitica O:9, Salmonella typhimurium, and Francisella tularensis. It has been shown that interaction of monoclonal antibodies and LPS of Brucella species can be critically dependent from buffer system. Obtained monoclonal antibodies allowed to develop highly sensitive assay which was able to detect antigens of Brucella species in concentrations 0.05 - 0.1 ng/ml. The assay can be used for detection and identification of Brucella species. 相似文献
10.
Although stress can alter the susceptibility of patients and animal models to convulsive epilepsy, little is known about the role of stress and glucocorticoid hormones in absence epilepsy. We measured the basal and acute stress-induced (foot-shocks: FS) concentrations of corticosterone in WAG/Rij rats, non-epileptic inbred ACI rats and outbred Wistar rats. The WAG/Rij strain is a genetic model for absence epilepsy and comorbidity for depression, which originates from the population of Wistar rats and, therefore, shares their genetic background. In a separate experiment, WAG/Rij rats were exposed to FS on three consecutive days. Electroencephalograms (EEGs) were recorded before and after FS, and the number of absence seizures (spike-wave-discharges, SWDs) was quantified. Both WAG/Rij rats and ACI rats exhibited elevated basal levels of corticosterone and a rapid corticosterone increase in response to acute stress. The WAG/Rij rats also displayed the most rapid normalization of corticosterone during the recovery phase compared to that of ACI and Wistar rats. FS had a biphasic effect on SWDs; an initial suppression was followed by an aggravation of the SWDs. By the third day, this aggravation of seizures was present in the hour preceding FS. This increase in SWDs may arise from anticipatory stress about the upcoming FS. Together, these results suggest that the distinct secretion profile of corticosterone found in WAG/Rij rats may contribute to the severity of the epileptic phenotype. Although the acute stressor results in an initial suppression of SWDs followed by an increase in SWDs, stress prior to a predictable negative event aggravates absences. 相似文献