排序方式: 共有10条查询结果,搜索用时 218 毫秒
1
1.
【背景】微生物脱硫是脱除气体中硫化氢的一种有效方法,其中,硫颗粒的生成与代谢是控制生物脱硫效率的关键,但目前相应的控制方法很少。【目的】研究不同种类表面活性剂对硫碱弧菌D301生成及利用硫颗粒的影响。【方法】通过摇床培养,利用X射线衍射、冷场发射扫描电镜、能谱分析及傅里叶红外光谱对硫颗粒进行表征。【结果】单质硫主要以S8形式存在,吐温-80和十二烷基磺酸钠(Sodium Dodecyl Sulfate,SDS)的添加对硫颗粒的形态及生成量影响明显。对照组中生成的硫颗粒呈规则球形,光滑完整,其表面附着蛋白质等生物大分子;加入0.01 g/L吐温-80后,硫颗粒呈长杆状、颗粒增大、利用速率减慢;加入0.3g/L的SDS后,硫颗粒呈短棒状、生成量减少、利用速率加快,同时延缓了硫碱弧菌D301的衰亡。【结论】添加表面活性剂可以改变硫颗粒形态并且影响其利用,是一种调控硫颗粒生成和代谢的有效手段。 相似文献
2.
Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria
Zheng Chen Gama Yang Xuemi Hao Nadia A. Samak Yunpu Jia Sumit Peh Tingzhen Mu Maohua Yang Jianmin Xing 《Engineering in Life Science》2021,21(10):693
Biological desulfurization offers several remarkably environmental advantages of operation at ambient temperature and atmospheric pressure, no demand of toxic chemicals as well as the formation of biologically re‐usable sulfur (S0), which has attracted increasing attention compared to conventionally physicochemical approaches in removing hydrogen sulfide from sour gas. However, the low biomass of SOB, the acidification of process solution, the recovery of SOB, and the selectivity of bio‐S0 limit its industrial application. Therefore, more efforts should be made in the improvement of the BDS process for its industrial application via different research perspectives. This review summarized the recent research advances in the microbial capture of hydrogen sulfide from sour gas based on strain modification, absorption enhancement, and bioreactor modification. Several efficient solutions to limitations for the BDS process were proposed, which paved the way for the future development of BDS industrialization. 相似文献
3.
Tingzhen Mu Maohua Yang Jixiang Zhao Moustafa Mohammed Sharshar Jiangnan Tian Jianmin Xing 《Biotechnology letters》2017,39(3):447-452
Objective
To construct efficient transformation and expression system and further improve desulfurizing activity of cells through expression of Vitreoscilla hemoglobin (VHb) in haloalkaliphilic Thialkalivibrio versutus SOB306.Results
We transferred plasmids pKT230 and pBBR-smr into T. versutus SOB306 via a conjugation method. We identified four promoters from among several predicted promoters by scoring for streptomycin resistance, and finally selected tac and p3 based on the efficiency of expression of red fluorescent protein (RFP). Expression of RFP when regulated by tac was more than three times that of p3 in SOB306. Further, we expressed VHb under the control of tac promoter in SOB306. Expression of VHb was verified using CO-difference spectra. The results showed that VHb expression can boost sulfur metabolism, as evidenced by an increase of about 11.7 ± 1.8% in the average rate of thiosulfate removal in the presence of VHb.Conclusion
A conjugation transfer and an expression system for Thialkalivibrio, has been developed for the first time and used for expression of VHb to improve desulfurizing activity.4.
Olajuyin Ayobami Matthew Yang Maohua Mu Tingzhen Tian Jiangnan Thygesen Anders Adesanoye Omolola Abidemi Adaramoye Oluwatosin Adekunle Song Andong Xing Jianmin 《Bioprocess and biosystems engineering》2018,41(10):1497-1508
Bioprocess and Biosystems Engineering - A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem... 相似文献
5.
生物脱硫是利用微生物脱除气体和石油中的含硫化合物,具有操作条件温和、工艺流程简单、脱硫效率高、能量消耗低和环境污染少等优点。但是,当前仍然缺乏简单高效的分析方法来定量分析生物脱硫过程中的含硫化合物。针对这个问题,建立了柱前荧光衍生高效液相色谱法同时测定生物脱硫溶液中的亚硫酸盐、硫代硫酸盐和硫化物的分析方法。该分析方法中含硫化合物的标准曲线具有良好的线性关系,亚硫酸盐、硫代硫酸盐和硫化物相关系数分别为0.999 46、0.999 67和0.999 65,其检测限分别为0.000 6 μmol/L、0.000 7 μmol/L和0.001 1 μmol/L;含硫化合物的加标回收率范围分别为98.17%–101.92%、100.90%–102.60%和101.11%–104.22%;并具有良好的重复性和稳定性。实验证明,该分析方法预处理简单、分析快速、结果准确,可用于同时测定不同生物脱硫系统中的含硫化合物。 相似文献
6.
Huacheng Cai Qian Wang Jingdong Gao Chunyan Li Xuemei Du Baopeng Ding Tingzhen Yang 《Physiology and Molecular Biology of Plants》2021,27(9):1997
Apple is considered the most commonly grown fruit crop in temperate regions that brings great economic profits to fruit growers. Dwarfing rootstocks have been extensively used in apple breeding as well as commercial orchards, but the molecular and genetic basis of scion dwarfing and other morphological traits induced by them is still unclear. At present, we report a genetic map of Malusdomestica × Malus baccata with high density. The F1 population was sequenced by a specific length amplified fragment (SLAF). In the genetic map, 5064 SLAF markers spanning 17 linkage groups (LG) were included. Dwarf-related and other phenotypic traits of the scion were evaluated over a 3-year growth period. Based on quantitative trait loci (QTL) evaluation of plant height and trunk diameter, two QTL clusters were found on LG 11, which exhibited remarkable influences on dwarfing of the scion. In this analysis, QTL DW2, which was previously reported as a locus that controls dwarfing, was confirmed. Moreover, three novel QTLs for total flower number and branching flower number were detected on LG2 and LG4, exhibited the phenotypic variation that has been explained by QTL ranging from 8.80% to 34.80%. The findings of the present study are helpful to find scion dwarfing and other phenotypes induced by rootstock in the apple.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12298-021-01069-0. 相似文献
7.
Tingzhen Mu Jixiang Zhao Yueping Guan Jiangnan Tian Maohua Yang Chen Guo Jianmin Xing 《Biotechnology letters》2017,39(6):865-871
Objective
Thialkalivibrio versutus D301 cells were immobilized on Fe3O4 nanoparticles (NPs) synthesized by an improved chemical coprecipitation method and modified with 3-aminopropyltriethoxysilane (APTES), then the immobilized cells were used in sulfur oxidation.Results
The prepared Fe3O4–APTES NPs had a narrow size distribution (10 ± 2 nm) and were superparamagnetic, with a saturation magnetization of 60.69 emu/g. Immobilized cells had a saturation magnetization of 34.95 emu/g and retained superparamagnetism. The optimum conditions for cell immobilization were obtained at pH 9.5 and 1 M Na+. The immobilization capacity of Fe3O4–APTES NPs was 7.15 g DCW/g-NPs that was 2.3-fold higher than that of Fe3O4 NPs. The desulfurization efficiency of the immobilized cells was close to 100%, having the same sulfur oxidation capacity as free cells. Further, the immobilized cells could be reused at least eight times, retaining more than 85% of their desulfurization efficiency.Conclusion
Immobilization of cells with the modified magnetic NPs efficiently increased cell controllability, have no effect on their desulfurization activity and could be effectively used in large-scale industrial applications.8.
9.
Jia Yang Xiawei Shi Rundi Gao Liming Fan Ruilin Chen Yu Cao Tingzhen Xu Junchao Yang 《Journal of cellular and molecular medicine》2023,27(23):3717-3728
To investigate the effect and mechanism of polydatin on bleomycin (BLM)-induced pulmonary fibrosis in a mouse model. The lung fibrosis model was induced by BLM. The contents of TNF-α, LPS, IL-6 and IL-1β in lung tissue, intestine and serum were detected by ELISA. Gut microbiota diversity was detected by 16S rDNA sequencing; R language was used to analyse species composition, α-diversity, β-diversity, species differences and marker species. Mice were fed drinking water mixed with four antibiotics (ampicillin, neomycin, metronidazole, vancomycin; antibiotics, ABx) to build a mouse model of ABx-induced bacterial depletion; and faecal microbiota from different groups were transplanted into BLM-treated or untreated ABx mice. The histopathological changes and collagen I and α-SMA expression were determined. Polydatin effectively reduced the degree of fibrosis in a BLM-induced pulmonary fibrosis mouse model; BLM and/or polydatin affected the abundance of the dominant gut microbiota in mice. Moreover, faecal microbiota transplantation (FMT) from polydatin-treated BLM mice effectively alleviated lung fibrosis in BLM-treated ABx mice compared with FMT from BLM mice. Polydatin can reduce fibrosis and inflammation in a BLM-induced mouse pulmonary fibrosis model. The alteration of gut microbiota by polydatin may be involved in the therapeutic effect. 相似文献
10.
1