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于1986—1988年,先后人工诱导2尾红镜鲤和4尾荷包红鲤抗寒品系雌核发育,获得雌核发育2倍体当年鱼种2133尾,其中红镜鲤685尾,荷包红鲤1448尾。通过对这些后代鳞被和体色两个质量性状的分析,发现荷包红鲤抗寒品系雌核发育2倍体(1987)中有约三分之一个体表现散鳞型鳞被和桔红色(出现由深到浅一系列变化)及少量桔黄色、肉红色个体。红镜鲤散鳞型鳞被出现体表全部覆盖不规则大型鳞片、2/3、1/2覆盖不规则的大型鳞片和散鳞型个体。体色出现桔红色、桔黄色,上述二色都出现由深到浅一系列变化和肉红色。这两个质量性状在雌核发育代中表现出数量性状的特征。  相似文献   
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Zhou  Lei  Sun  Shiwei  Zhang  Tieqi  Yu  Yueming  Xu  Liang  Li  Haoran  Wang  Minghai  Hong  Yang 《Molecular biology reports》2020,47(10):7439-7449
Molecular Biology Reports - The dysfunction of bone marrow mesenchymal stem cells (BMSCs) in balancing osteogenesis and adipogenesis plays an important role in the occurrence and development of...  相似文献   
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Poor quality and insufficient productivity are two main obstacles for the practical application of graphene in electrochemical energy storage. Here, high‐quality crumpled graphene microflower (GmF) for high‐performance electrodes is designed. The GmF possesses four advantages simultaneously: highly crystallized defect‐free graphene layers, low stacking degree, sub‐millimeter continuous surface, and large productivity with low cost. When utilized as carbon host for sulfur cathode, the GmF‐sulfur hybrid delivers decent areal capacities of 5.2 mAh cm?2 at 0.1 C and 3.8 mAh cm?2 at 0.5 C. When utilized as cathode of Al‐ion battery, the GmF affords a high capacity of 100 mAh g?1 with 100% capacity retention after 5000 cycles and excellent rate capability from 0.1 to 20 A g?1. This facile and large‐scale producible GmF represents a meaningful high‐quality graphene powder for practical energy storage technology. Meanwhile, this unique high‐quality graphene design provides an effective route to improve electrochemical properties of graphene‐based electrodes.  相似文献   
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Objective:In bone tissue engineering, the use of osteoblastic seed cells has been widely adopted to mediate the osteogenic differentiation so as to prompt bone regeneration and repair. It is hypothesized that Dok5 can regulate the proliferation and differentiation of osteoblasts. In this study, the role of Dok5 in osteoblast proliferation and differentiation was investigated.Methods:A lentiviral vector to silence Dok5 was transferred to C3H10, 293T and C2C12 cells. CCK-8 assay was used to detect the cell proliferation. Cells were stained by ALP and AR-S staining. Western blot and RT-PCR were used to detect the expression levels of related factors.Results:Dok5 expression level was gradually up-regulated during the osteoblast differentiation. Dok5 silencing down-regulated the expression levels of osteogenic biosignatures OPN, OCN, and Runx2 and suppressed the osteogenesis. Additionally, the osteoblast proliferation and canonical Wnt/β-catenin signaling were suppressed upon Dok5 knockdown, β-catenin expression level was significantly down-regulated in the knockdown group, while the expression levels of GSK3-β and Axin, negative regulators in the Wnt signaling pathway, were up-regulated. Furthermore, overexpression of Dok5 promoted the proliferation and osteogenesis and activated the canonical Wnt/β-catenin signaling pathway.Conclusion:Dok5 may regulate the osteogenic proliferation and differentiation via the canonical Wnt/β-catenin signaling pathway.  相似文献   
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固体发酵法生产α—淀粉酶的研究   总被引:4,自引:0,他引:4  
固体发酵是一种有效的α-淀粉酶的生产方法,它的淀粉酶产酶水平较高,能达1500μ/g,发酵周期60h,发酵培养基为麸皮。  相似文献   
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细菌肥料活菌发酵增长因子的研究   总被引:3,自引:0,他引:3  
豆科、桔梗科一些植物果实含有刺激微生物细胞分裂、促进细胞增长的增长因子。本所经大量初筛,获得一种对固氮菌和大豆根瘤菌刺激生长效果显著的增长因子Ⅱ,使固氮菌(Alcaligensfaecalis)发酵最高菌数提高3倍,发酵周期缩短30%,大豆根瘤(22—10)菌数提高5倍。经分析,增长因子Ⅱ的主要成份为6-甲氧基-7-氢基香豆素.  相似文献   
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药敏药片临床应用观察报告   总被引:1,自引:0,他引:1  
本文报导省市级三所医院同时用药敏药片和药敏纸片对国际标准质株做抑菌试验,其结果:药片片重差变异系数cv=2.5,纸片cv=14.4.抽样五种抗菌素抑菌环直径差变异系数分别为:药片平均cv=3.07,而纸片平均cv=6.85;均匀度L药片为2-3mm纸片为3-5mm.表明药片片间差小,均匀度、准确度合格,药物含量均匀,精密度好于纸片.  相似文献   
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