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排序方式: 共有129条查询结果,搜索用时 15 毫秒
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Yuxin Wu Lei Wang Chen Lin Yan Lin Mei Zhou Liang Chen Brian Connolly Yingqi Zhang Tianbao Chen Chris Shaw 《PloS one》2013,8(2)
The defensive skin secretions of amphibians are a rich resource for the discovery of novel, bioactive peptides. Here we report the identification of a novel vascular smooth muscle-relaxing peptide, named vasorelaxin, from the skin secretion of the Chinese piebald odorous frog, Odorrana schmackeri. Vasorelaxin consists of 20 amino acid residues, SRVVKCSGFRPGSPDSREFC, with a disulfide-bridge between Cys-6 and Cys-20. The structure of its biosynthetic precursor was deduced from cloned skin cDNA and consists of 67 amino acid residues encoding a single copy of vasorelaxin (vasorelaxin, accession number: ). Synthetic vasorelaxin caused a profound relaxation of rat arterial smooth muscle with an EC50 of 6.76 nM. HE860494相似文献
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Xiaole Chen He Wang Lei Wang Mei Zhou Tianbao Chen Chris Shaw 《The protein journal》2016,35(4):291-299
The chemical compounds synthesised and secreted from the dermal glands of amphibian have diverse bioactivities that play key roles in the hosts’ innate immune system and in causing diverse pharmacological effects in predators that may ingest the defensive skin secretions. As new biotechnological methods have developed, increasing numbers of novel peptides with novel activities have been discovered from this source of natural compounds. In this study, a number of defensive skin secretion peptide sequences were obtained from the European edible frog, P. kl. esculentus, using a ‘shotgun’ cloning technique developed previously within our laboratory. Some of these sequences have been previously reported but had either obtained from other species or were isolated using different methods. Two new skin peptides are described here for the first time. Esculentin-2c and Brevinin-2Tbe belong to the Esculentin-2 and Brevinin-2 families, respectively, and both are very similar to their respective analogues but with a few amino acid differences. Further, [Asn-3, Lys-6, Phe-13] 3-14-bombesin isolated previously from the skin of the marsh frog, Rana ridibunda, was identified here in the skin of P. kl. esculentus. Studies such as this can provide a rapid elucidation of peptide and corresponding DNA sequences from unstudied species of frogs and can rapidly provide a basis for related scientific studies such as those involved in systematic or the evolution of a large diverse gene family and usage by biomedical researchers as a source of potential novel drug leads or pharmacological agents. 相似文献
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Lu T Markotan T Coppo F Tomczuk B Crysler C Eisennagel S Spurlino J Gremminger L Soll RM Giardino EC Bone R 《Bioorganic & medicinal chemistry letters》2004,14(14):3727-3731
Through structure-based drug design and parallel synthesis, we have discovered a novel series of nonpeptidic phenyl-based thrombin inhibitors using oxyguanidines as guanidine bioisosteres. These compounds have been found to be highly potent, highly selective, and orally bioavailable. 相似文献
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Bradykinin and (Thr(6))-bradykinin have been identified in the defensive skin secretion of the fire-bellied toad, Bombina orientalis. The homologous cDNAs for both peptides were cloned from a skin library using a 3'- and 5'-RACE strategy. Kininogen-1 (BOK-1) contained an open-reading frame of 167 amino acid residues encoding four repeats of bradykinin, and kininogen-2 (BOK-2) contained an open-reading frame of 161 amino acid residues encoding two repeats of (Thr(6))-bradykinin. Alignment of both precursor nucleotide and amino acid sequences revealed a high degree of structural similarity. These amphibian skin kininogens/preprobradykinins are not biologically analogous to mammalian kininogens. 相似文献
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The structural diversity of polypeptides in amphibian skin secretion probably reflects different roles in dermal regulation or in defense against predators. Here we report the structures of two novel trypsin inhibitor analogs, BOTI and BVTI, from the dermal venom of the toads, Bombina orientalis and Bombina variegata. Cloning of their respective precursors was achieved from lyophilized venom cDNA libraries for the first time. Amino acid alignment revealed that both deduced peptides, consisting of 60 amino acid residues, including 10 cysteines and the reactive center motif, -CDKKC-, can be affirmed as structural homologs of the trypsin inhibitor from Bombina bombina skin. 相似文献
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Shuli Yang Songcheng Ma Jing Chen Huaming Mao Yiduo He Dongmei Xi Liangyu Yang Tianbao He Weidong Deng 《Molecular biology reports》2010,37(4):2063-2073
Libraries of rumen bacterial 16S rRNA gene sequences of Gayals (Bos frontalis) and Swamp buffaloes (Bubalus bubalis) were cloned and sequenced in the present work to compare the bacterial diversity with the third published library of Holstein
cow. Sequence similarity of 97% was used as the definition of operational taxonomic unit (OTU). The majority of the 470 sequences
retrieved fell into the phyla of low G + C subdivision (329 sequences) and Cytophaga–Flexibacter–Bacteroides (CFB, 123 sequences) with the percentages of 70 and 26.2, respectively. The remaining clones belonged to the phyla of Proteobacter, high G + C gram positive bacteria (HGCGPB) and Spirochaetes, accounting for 3.8% totally. Only 73 clones (25 OTUs, 15.5%) could be closely related to cultured representatives. However,
a larger fraction was related to uncultured representatives. Holstein cow may have more representatives of cultural bacteria
and there were more uncultured clones for Gayals. The percentage of cultural representatives was 24, 13.3 and 9.5 for Holstein
cow, Swamp buffaloes and Gayals, respectively. Twenty-three OTUs of the 236 ones appeared in more than one library, five of
which were cultural. Selenomonas ruminantium, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens were found in two different libraries, while Succiniclasticum
ruminis and Pseudobutyrivibrio ruminis were found in all three libraries. Some of the animal-specific bacteria that had not been described previously in the ruminal
ecosystem, e.g. Allisonella histaminiformans for Gayals and Staphylococcus sciuri for Swamp buffaloes were also recovered. 相似文献
8.
RpoS蛋白是RNA聚合酶的一种σ因子,能够调控一组特异性基因的表达,因此在细菌中发挥着至关重要的作用.在细菌中,RpoS蛋白的表达受到严格的控制,主要表现在3个水平的调控:转录水平,翻译水平和翻译后水平.环境应力作用通过信号传导进入细菌细胞内,引起一系列微环境的改变,进而引起调控子的变化.通过调控于与RpoS蛋白直接和间接的相互作用,达到控制其水平的目的.另外,由于RpoS蛋白在细菌中有特殊作用,因此RpoS蛋白水平的变化会引起众多基因表达水平的改变,从而引起细菌对不同环境应力应答的变化以及细菌的某些特性的改变,为今后细菌病害的防治提供了新的策略.综述了近年来对RpoS蛋白的表达调控以及在几种常见菌种中功能的研究进展,由于RpoS蛋白的功能的复杂性,仍有大量的未知功能有待进一步鉴定. 相似文献
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