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1.
G A Dunaway G L Leung J R Thrasher M D Cooper 《The Journal of biological chemistry》1978,253(20):7460-7463
Earlier work demonstrated that the activity of liver phosphofructokinase (PFK-L2) and immunoreactive PFK-L2 were decreased in diabetic rats and increased to normal or super-normal amounts following insulin treatment (Dunaway, G.A., and Weber, G., (1974) Arch. Biochem. Biophys. 162, 629-637). This report indicates that the decrease in levels of PFK-L2 in diabetic rats is a result of an accelerated degradation rate while the synthetic rate remains nearly normal. Following insulin treatment, the rate of PFK-L2 synthesis is enhanced 2-fold, whereas the rate of degradation appears to be greatly diminished. An inverse relationship is shown to exist between the PFK-L2 levels and the rates of PFK-L2 degradation, suggesting that the levels of PFK-L2 are primarily regulated by degradation rate. In addition, the levels of the PFK-L2 peptide stabilizing factor are inversely proportional to rates of PFK-L2 degradation. These results indicate that insulin mediates the rate of degradation of PFK-L2 by controlling the level of the peptide stabilizing factor. 相似文献
2.
Characterization of the interaction of the glp repressor of Escherichia coli K-12 with single and tandem glp operator variants. 总被引:2,自引:2,他引:0 下载免费PDF全文
N Zhao W Oh D Trybul K S Thrasher T J Kingsbury T J Larson 《Journal of bacteriology》1994,176(8):2393-2397
The glp operons of Escherichia coli are negatively controlled by the glp repressor. Comparison of the repressor-binding affinities for consensus and altered consensus operators in vivo showed that all base substitutions at positions 3, 4, 5, and 8 from the center of the palindromic operator caused a striking decrease in repressor binding. Substitutions at other positions had a severe to no effect on repressor binding, depending on the base substitution. The results obtained indicate that the repressor binds with highest affinity to operators with the half-site WATKYTCGWW, where W is A or T, K is G or T, and Y is C or T. Strong cooperative binding of the repressor to tandem operators was demonstrated in vivo. Cooperativity was maximal when two 20-bp operators were directly repeated or when 2 bp separated the two operators. Cooperativity decreased with the deletion of 2 bp or the addition of 4 bp between the individual operators. Cooperativity was eliminated with a 6-bp insertion between the operators. 相似文献
3.
David D. Gillette Óscar Carranza-Castañeda Richard S. WhiteJr Gary S. Morgan Larry C. Thrasher Robert McCord Gavin McCullough 《Journal of Mammalian Evolution》2016,23(2):133-154
North American glyptodonts originated from South American ancestors during the Great American Biotic Interchange no later than early Blancan North American Land Mammal Age (NALMA). A substantial expansion in population samples from the late Blancan 111 Ranch fauna of southeastern Arizona, several late Blancan faunas in New Mexico, and the early Blancan–Irvingtonian faunas of Guanajuato, Mexico, permit, analysis of sexual dimorphism and ontogeny of Glyptotherium texanum Osborn, 1903. Growth of carapacial osteoderms was allometric, including changes of the external sculpturing. Overall anatomy of the carapace changed with growth, with development of distinctive pre-iliac and post-iliac regions in lateral profile of adults. Skulls of adults possess a unique boss on the anterior surface of the descending process of the zygomatic arch that is not present in juveniles. Sexual dimorphism involves differences in anatomy of lateral and posterior osteoderms. Glyptotherium arizonae Gidley, 1926, is a junior synonym of G. texanum. The temporal distribution of G. texanum extends from early Blancan NALMA to Irvingtonian NALMA, with geographical distribution from Central America and Mexico to southern United States. 相似文献
4.
Thrasher TN 《American journal of physiology. Regulatory, integrative and comparative physiology》2005,288(4):R863-R871
We have developed a new model of chronic baroreceptor unloading (CBU) in the dog. Initial characterization of the model indicated that CBU increased mean arterial pressure (MAP) by an average of 22 mmHg for 7 days. The goal of the present study was to replicate the previous study using telemetry to record MAP continuously and to determine the effects of CBU (n = 7) on chronic regulation of MAP. We also prepared a group of dogs with sinoaortic denervation (SAD, n = 6) to compare the time course of changes in MAP in the two models. Control levels (7 day average +/- SE) of MAP in the CBU and SAD groups were 94 +/- 2 and 94 +/- 1 mmHg, respectively. MAP averaged 124 +/- 8 and 103 +/- 4 mmHg during the first and second weeks after SAD (both P < 0.05) and then declined to levels not different from control during weeks 3-5. In the CBU group, MAP averaged 120 +/- 4 mmHg during the first week, declined to 111 +/- 4 mmHg during the second week, and stabilized at 104 mmHg during weeks 3-5 (all P < 0.05 compared with control). Plasma norepinephrine levels were increased significantly for the first week after SAD and for 2 wk after CBU but were not different from control for the remainder of the study. These results indicate that the initial increase in MAP after CBU is not sustained but declines to a level that is modestly higher than control. However, because MAP did not fall to control levels, the results are compatible with the hypothesis that baroreceptor input can influence the long-term level of MAP. 相似文献
5.
Infants born with severe combined immune deficiencies are prone to life-threatening infections and, without treatment, do not survive beyond the first year of life. Haematopoietic stem cell transplantation from a fully matched donor offers the possibility of cure. In the absence of a suitable matched donor, haploidentical transplants from a parental donor may be undertaken, but these are associated with more complications and lower success rates. Recently, an alternative therapeutic option based on retroviral gene delivery has been used to correct X-linked severe combined immune deficiency (SCID-X1) and adenosine deaminase deficiency. Clinical trials have established that in situations where ex vivo gene transfer into haematopoietic progenitor cells confers a strong selective advantage, the procedure is a feasible alternative to haploidentical transplantation, with favourable kinetics of immune reconstitution. 相似文献
6.
Thrasher AJ 《Nature reviews. Immunology》2002,2(9):635-646
The regulation of many immunological events depends on systems that mediate dynamic actin reorganization in response to signals from the cell membrane. The Wiskott-Aldrich syndrome protein (WASp) is the founding member of a family of proteins that have emerged as crucial effectors of Rho GTPases and activators of the cytoskeletal-organizing complex Arp2/3. Now, WASp has been shown to be intimately involved in many pathways that influence the function of the immune system. Disturbances in these systems result in the complex immunodysregulation of Wiskott-Aldrich syndrome. 相似文献
7.
Myeloid leukocytes are the first line of host defence. When they sense perturbations in tissue homeostasis such as infection, inflammation and ischemia, they respond by trafficking. Whilst neutrophils and macrophages migrate to sites of infection, dendritic cells (DC) migrate from tissue-resident sites back into lymph nodes where they activate T and B lymphocytes. The directed migration of these leukocytes through peripheral tissues is thus crucial for their function. This article considers recent advances in our understanding of the adhesive and motile behaviour of macrophages and DC, with particular emphasis on the podosomes that appear to be required for normal migration through extracellular matrices. 相似文献
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10.
Harnessing parallelism in multicore clusters with the All-Pairs,Wavefront, and Makeflow abstractions
Li Yu Christopher Moretti Andrew Thrasher Scott Emrich Kenneth Judd Douglas Thain 《Cluster computing》2010,13(3):243-256
Both distributed systems and multicore systems are difficult programming environments. Although the expert programmer may
be able to carefully tune these systems to achieve high performance, the non-expert may struggle. We argue that high level
abstractions are an effective way of making parallel computing accessible to the non-expert. An abstraction is a regularly
structured framework into which a user may plug in simple sequential programs to create very large parallel programs. By virtue
of a regular structure and declarative specification, abstractions may be materialized on distributed, multicore, and distributed
multicore systems with robust performance across a wide range of problem sizes. In previous work, we presented the All-Pairs
abstraction for computing on distributed systems of single CPUs. In this paper, we extend All-Pairs to multicore systems,
and introduce the Wavefront and Makeflow abstractions, which represent a number of problems in economics and bioinformatics.
We demonstrate good scaling of both abstractions up to 32 cores on one machine and hundreds of cores in a distributed system. 相似文献