全文获取类型
收费全文 | 1759篇 |
免费 | 153篇 |
专业分类
1912篇 |
出版年
2024年 | 2篇 |
2023年 | 8篇 |
2022年 | 24篇 |
2021年 | 34篇 |
2020年 | 21篇 |
2019年 | 26篇 |
2018年 | 29篇 |
2017年 | 34篇 |
2016年 | 38篇 |
2015年 | 87篇 |
2014年 | 86篇 |
2013年 | 97篇 |
2012年 | 159篇 |
2011年 | 147篇 |
2010年 | 101篇 |
2009年 | 78篇 |
2008年 | 119篇 |
2007年 | 117篇 |
2006年 | 93篇 |
2005年 | 106篇 |
2004年 | 80篇 |
2003年 | 89篇 |
2002年 | 85篇 |
2001年 | 11篇 |
1999年 | 20篇 |
1998年 | 24篇 |
1997年 | 18篇 |
1996年 | 7篇 |
1995年 | 20篇 |
1994年 | 13篇 |
1993年 | 10篇 |
1992年 | 15篇 |
1991年 | 9篇 |
1990年 | 6篇 |
1989年 | 10篇 |
1988年 | 9篇 |
1987年 | 8篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 11篇 |
1981年 | 8篇 |
1980年 | 4篇 |
1978年 | 4篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1971年 | 3篇 |
1935年 | 1篇 |
排序方式: 共有1912条查询结果,搜索用时 0 毫秒
1.
2.
3.
The aggregation of amyloid-β protein (Aβ) in vivo is a critical pathological event in Alzheimer's disease. Although more and more evidence shows that the intermediate oligomers are the primary neurotoxic species in Alzheimer's disease, the particular structural features responsible for the toxicity of these intermediates are poorly understood. We measured the peptide level solvent accessibility of multiple Aβ(1-40) aggregated states using hydrogen exchange detected by mass spectrometry. A gradual reduction in solvent accessibility, spreading from the C-terminal region to the N-terminal region was observed with ever more aggregated states of Aβ peptide. The observed hydrogen exchange protection begins with reporter peptides 20-34 and 35-40 in low molecular weight oligomers found in fresh samples and culminates with increasing solvent protection of reporter peptide 1-16 in long time aged fibrillar species. The more solvent exposed structure of intermediate oligomers in the N-termini relative to well-developed fibrils provides a novel explanation for the structure-dependent neurotoxicity of soluble oligomers reported previously. 相似文献
4.
Theresa M. Bert Kevin J. McCarthy Hector Cruz-Lopez Steven Bogdanowicz 《Evolution; international journal of organic evolution》1996,50(2):655-671
Many investigators categorize individuals from hybrid zones to facilitate comparisons among genotypic classes (e.g., parental, F1, backcross) for comparative studies in which components of fitness or geographic variation are being analyzed. Frequently, multiple character sets representing genetically independent traits are used to classify these individuals and various methodologies are employed to combine the classifications obtained from the different character sets. We adapted the principles of total evidence and taxonomic congruence (two formalized approaches used by systematists in formulating phylogenetic hypotheses) to address the problem of discriminating hybridizing species and classifying individuals from hybrid zones. As our model, we used two morphological (coloration and morphometric) and two molecular (allozyme and mitochondrial DNA restriction-fragment-length polymorphism) character sets that differentiate two stone crab species (Menippe adina and M. mercenaria). Using principal-components analysis, we determined that combining character sets and eliminating characters or character sets that did not have large eigenvector coefficients for the principal component that best separated the two species yielded the highest level of discrimination between species and allowed us to classify a broad range of morpho-genotypes as hybrids. For the stone crabs, three diagnostic allozyme loci and five diagnostic coloration characters best separated the species. The two character sets were not completely congruent, but they agreed in their classification of 50% of the individuals from the hybrid zone and rarely strongly disagreed in their classifications. Classification discrepancies between the two character sets probably represent variation between traits in interspecific gene flow rather than intraspecific, ecologically mediated variation. Our results support the assertions of previous investigators who espoused the benefits associated with using multiple character sets to classify individuals from hybrid zones and demonstrate that, if character sets are reasonably congruent and numerically balanced, combining diagnostic characters from multiple character sets (a total-evidence approach) can enhance discriminatory power between species and facilitate the assignment of hybrid-zone individuals to genotypic classes. On the contrary, classifying hybrid-zone individuals using character sets separately (a taxonomic-congruence approach) provides the opportunity to compare levels of introgression between species and to assess reasons for discordance among the data sets. 相似文献
5.
6.
Suppression by the ColV,I-K94 plasmid of a growth lesion in ompA mutants of Escherichia coli 总被引:1,自引:0,他引:1
Organisms of three independently isolated ompA mutants of Escherichia coli failed to form colonies on glucose minimal agar (glucose MA) at 44 degrees C after growth in glucose minimal salts medium at 37 degrees C, although all three strains formed colonies on nutrient agar at 44 degrees C. Supplementation of the glucose MA with individual amino acids including L-methionine and/or L-cysteine did not allow colony formation at 44 degrees C, although addition of 0.1% Casamino acids was effective; replacement of glucose with other energy sources or ammonium ions with glutamate also did not allow growth at 44 degrees C. The failure to form colonies at 44 degrees C was not due to killing of the organisms, because colonies were formed if plates of the ompA mutant initially incubated at 44 degrees C were shifted to 30 degrees C after 16 h. Introduction of the ColV, I-K94 plasmid into P678-54 ompA, 1131 ompA or an ompC ompA mutant suppressed the 44 degrees C growth lesion, but other plasmids (F lac, R483ColIa, RI, ColB-K98, R124) tested in P678-54 ompA did not. Growth of the ColV, I-K94+ derivative at 44 degrees C was due to a suppressing effect of the plasmid rather than to introduction of the plasmid into a variant with normal or altered OmpA protein. An attempt was made to ascertain which component(s) encoded by ColV, I-K94 was (were) responsible for allowing growth at 44 degrees C. Transfer components appeared unlikely to be involved and plasmids which conferred individual colicins (plus the corresponding immunity component) did not suppress.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
7.
8.
Effect of Enzymes on the Composition and Structure of Chromobacterium violaceum Cell Envelopes 总被引:2,自引:2,他引:0 下载免费PDF全文
Cell envelopes of Chromobacterium violaceum were isolated and treated under controlled conditions with trypsin, Pronase, lipase, phospholipase C, lysozyme, and a mixture of enzymes produced by a bacteriolytic Pseudomonas sp. After each enzyme treatment, losses in dry weight, protein, lipid, carbohydrate, 2,6-diaminopimelic acid, and total phosphorus were determined. Electron-microscopic examination of the enzyme-treated envelopes indicated complete or partial loss of envelope rigidity or some envelope fragmentation, or both. Each enzyme hydrolyzed at least one envelope component and liberated several others into the supernatant fluid, where they appeared as nondialyzable particulate components, identified by means of electron microscopy. Unlike the other enzymes, the Pseudomonas sp. enzyme mixture partially liberated all major envelope components except phosphorus, heptose, and 2-keto-3-deoxy octonic acid. In spite of these large losses, the envelopes preserved some features of their integrity and elongated shape. 相似文献
9.
A lectin preparation obtained fromTetracarpidium conophorum (Nigerian walnut) by affinity chromatography of seed extracts on lactose-agarose has been shown to contain two components by gel filtration on Sephadex G150. The larger componentTetracarpidium conophorum agglutinin I (TCAI) is a disulphide-bonded 70 kDa homodimer whereas the second component TCAII is a 34 kDa monomeric protein. Amino terminal aminoacid sequencing shows identity in TCAI and TCAII for the first fifteen residues after which the sequences diverge. TheN-terminal sequences of TCAI and TCAII show identity with sequences in the B-chains of ricin andRicinus communis agglutinin I (RCAI) in eleven of the initial fifteen residues. Thereafter TCAI appears to be homologous to the ricin B chain whereas TCAII is more homologous with the B chain of RCAI. A limited screening of the carbohydrate-binding specificity of TCAII by affinity chromatography of defined oligosaccharides on TCAII Sepharose columns shows that the binding specificity reported earlier for affinity purifiedTetracarpidium conophorum isolectins (Sato S, Animashaun T, Hughes RC (1991)J Biol Chem
266:11485–94) reflects the binding properties of TCAII which is the major isolectin in unfractionated lectin preparations. 相似文献
10.