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Summary The child with iminoglycinuria is in our observation. Hyperprolinuria was seen at 5 months by screening program. The child was repeatedly examined in the hospital and was seen last time at 16 months. IQ was 67, in the urine were excessive amounts of glycine, proline a hydroxyproline. In the blood aminoacids were in normal levels. In the child was noticed the same increase of proline in the blood as in the control child of the same age following loading test with L-proline, indicating normal intestinal absoption. Both parents and father's sister's 2-year-old mentally retarded child exhibit excessive glycinuria. The father, his sister, father's father, and grandfather are partialy, congenitaly deaf. 相似文献
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Summary A support based on pyrogeneous silicon dioxide of particle size 0.01 to 0.1/um, modified by 3-(amino)propyltriethoxysilane and activated by glutaraldehyde was employed for the immobilization of concanavalin A, immunoglobulins, basic pancreatic trypsin inhibitor, and chymotrypsin. Its binding capacity is comparable with that of porous supports while the biological activity of the proteins immobilized is retained. Nonspecific adsorption of these proteins to the support is low compared to its binding capacity. 相似文献
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Elžbieta Milewska-Pawliczuk 《Biologia Plantarum》1987,29(4):295-298
Six thousand anthers ofSecale cereale cv. Dańkowskie Zlote and inbred lines L214 L215 and L258c/5, in the uninuclear microspore were cultured over a period of
three months on Murashige and Skoog nutrient medium supplemented with IAA (1–2μg1−1), kinetin (0.2 to (1.0μg1−1) and 2,4-D (0.5–2.0μg1−1). First divisions of microspore were observed after 7 days. In the course of 10 weeks, 4 albino tic embryos at the cotyledon
stage were observed that died away in the course of further culture. The course of androgenesis was regular in inbred lines
and irregular in rye cv. Dańkowskie Ziote. The efficiency of androgenesis and the amount of observed globular structures in
anthers were also dependent on the genetic potency of the material. Inbred lines did not show any greater viability of embryos. 相似文献
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J. Šťovíčková J. Turková B. Urbánek V. Bažant J. Kavalírová 《Biotechnology Techniques》1988,2(2):121-126
Summary The effect was studied of the method of drying chymotrypsin attached to bead cellulose on its chemical and physical characteristics. These characteristics are not deteriorated when replacing the commonly used lyophilisation by fluid drying in the air stream. The preparations saved essentially the same proteolytic activity as well as stability even when increasing the drying air temperature to 70°C. 相似文献
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Sterol composition of a δ5,7-sterol-rich strain ofSaccharomyces cerevisiae during batch growth 总被引:1,自引:0,他引:1
Sterol composition was examined during batch growth on complex media containing ethanol, molasses or glucose as the carbon source. The molasses-grown cells exhibited a balanced sterol composition throughout growth, maintaining the proportion of ergosterol to 24:28-dehydroergosterol equal to 1.4. The negative effect of glucose on sterol synthesis manifested itself by decreasing the accumulation of 24:28-dehydroergosterol and total sterols but not of ergosterol. Using ethanol as the sole carbon source, a large amount of 24:28-dehydroergosterol accumulated, partly at the expense of other sterols. The gradual addition of nitrogen source during growth significantly decreased the accumulation of ergosterol, 24:28-dehydroergosterol and of total sterols. A general scheme of regulation of sterol synthesis in baker's yeast is presented. 相似文献
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In submerged Claviceps cultures the activity of hydroxymethylglutaryl-CoA reductase preceded the increase of alkaloid production and of sterol content. During the first alkaloid phase, cell mevalonate was involved in the biosynthesis of both alkaloids and steroids. In the second production phase, it was predominantly used for alkaloid synthesis. Hydroxymethylglutaryl-CoA reductase appears to be a suitable target for physiological manipulation to increase clavine alkaloid yields. 相似文献
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