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1.
Upright posture, standing up from a chair, and gait were analyzed in patients after one-sided total hip replacement and in healthy subjects (control). It was found that the patients predominantly loaded the unoperated leg when they stood quietly or rose from a chair. Subjects’ walking on a 10-m podograph treadmill showed that their walking speed was slower than that of healthy subjects and the swing phase on the side of hip replacement was longer than on the unoperated side. It was assumed that the unequal load on legs during walking, standing, and sit-to-stand performance in patients with total hip replacement was related to the sensory deficit of the artificial joint, leading to the overstrain of the unoperated leg and coxarthrosis in it.  相似文献   
2.
The purpose of a drinking water distribution system is to deliver drinking water to the consumer, preferably with the same quality as when it left the treatment plant. In this context, the maintenance of good microbiological quality is often referred to as biological stability, and the addition of sufficient chlorine residuals is regarded as one way to achieve this. The full-scale drinking water distribution system of Riga (Latvia) was investigated with respect to biological stability in chlorinated drinking water. Flow cytometric (FCM) intact cell concentrations, intracellular adenosine tri-phosphate (ATP), heterotrophic plate counts and residual chlorine measurements were performed to evaluate the drinking water quality and stability at 49 sampling points throughout the distribution network. Cell viability methods were compared and the importance of extracellular ATP measurements was examined as well. FCM intact cell concentrations varied from 5×103 cells mL−1 to 4.66×105 cells mL−1 in the network. While this parameter did not exceed 2.1×104 cells mL−1 in the effluent from any water treatment plant, 50% of all the network samples contained more than 1.06×105 cells mL−1. This indisputably demonstrates biological instability in this particular drinking water distribution system, which was ascribed to a loss of disinfectant residuals and concomitant bacterial growth. The study highlights the potential of using cultivation-independent methods for the assessment of chlorinated water samples. In addition, it underlines the complexity of full-scale drinking water distribution systems, and the resulting challenges to establish the causes of biological instability.  相似文献   
3.
Talis  V. L. 《Human physiology》2021,47(3):249-253
Human Physiology - The notions on neuromuscular tone are discussed up from the time of N.A.Bernstein, who regarded it as level A, Rubro-Spinal Level of Paleokinetic Regulations in his book On the...  相似文献   
4.
We examined differences in fine root morphology, mycorrhizal colonisation and root-inhabiting fungal communities between Picea abies individuals infected by Heterobasidion root-rot compared with healthy individuals in four stands on peat soils in Latvia. We hypothesised that decreased tree vitality and alteration in supply of photosynthates belowground due to root-rot infection might lead to changes in fungal communities of tree roots. Plots were established in places where trees were infected and in places where they were healthy. Within each stand, five replicate soil cores with roots were taken to 20 cm depth in each root-rot infected and uninfected plot. Root morphological parameters, mycorrhizal colonisation and associated fungal communities, and soil chemical properties were analysed. In three stands root morphological parameters and in all stands root mycorrhizal colonisation were similar between root-rot infected and uninfected plots. In one stand, there were significant differences in root morphological parameters between root-rot infected versus uninfected plots, but these were likely due to significant differences in soil chemical properties between the plots. Sequencing of the internal transcribed spacer of fungal nuclear rDNA from ectomycorrhizal (ECM) root morphotypes of P. abies revealed the presence of 42 fungal species, among which ECM basidiomycetes Tylospora asterophora (24.6 % of fine roots examined), Amphinema byssoides (14.5 %) and Russula sapinea (9.7 %) were most common. Within each stand, the richness of fungal species and the composition of fungal communities in root-rot infected versus uninfected plots were similar. In conclusion, Heterobasidion root-rot had little or no effect on fine root morphology, mycorrhizal colonisation and composition of fungal communities in fine roots of P. abies growing on peat soils.  相似文献   
5.
The E6 protein of the high-risk human papillomaviruses (HPVs) and the cellular ubiquitin-protein ligase E6AP form a complex which causes the ubiquitination and degradation of p53. We show here that HPV16 E6 promotes the ubiquitination and degradation of E6AP itself. The half-life of E6AP is shorter in HPV-positive cervical cancer cells than in HPV-negative cervical cancer cells, and E6AP is stabilized in HPV-positive cancer cells when expression of the viral oncoproteins is repressed. Expression of HPV16 E6 in cells results in a threefold decrease in the half-life of transfected E6AP. E6-mediated degradation of E6AP requires (i) the binding of E6 to E6AP, (ii) the catalytic activity of E6AP, and (iii) activity of the 26S proteasome, suggesting that E6-E6AP interaction results in E6AP self-ubiquitination and degradation. In addition, both in vitro and in vivo experiments indicate that E6AP self-ubiquitination results primarily from an intramolecular transfer of ubiquitin from the active-site cysteine to one or more lysine residues; however, intermolecular transfer can also occur in the context of an E6-mediated E6AP multimer. Finally, we demonstrate that an E6 mutant that is able to immortalize human mammary epithelial cells but is unable to degrade p53 retains its ability to bind and degrade E6AP, raising the possibility that E6-mediated degradation of E6AP contributes to its ability to transform mammalian cells.  相似文献   
6.
The formation of biofilms in drinking water distribution networks is a significant technical, aesthetic and hygienic problem. In this study, the effects of assimilable organic carbon, microbially available phosphorus (MAP), residual chlorine, temperature and corrosion products on the formation of biofilms were studied in two full-scale water supply systems in Finland and Latvia. Biofilm collectors consisting of polyvinyl chloride pipes were installed in several waterworks and distribution networks, which were supplied with chemically precipitated surface waters and groundwater from different sources. During a 1-year study, the biofilm density was measured by heterotrophic plate counts on R2A-agar, acridine orange direct counting and ATP-analyses. A moderate level of residual chorine decreased biofilm density, whereas an increase of MAP in water and accumulated cast iron corrosion products significantly increased biofilm density. This work confirms, in a full-scale distribution system in Finland and Latvia, our earlier in vitro finding that biofilm formation is affected by the availability of phosphorus in drinking water.  相似文献   
7.
Recently we introduced a new version of the perceptual retouch model incorporating two interactive binding operations—binding features for objects and binding the bound feature-objects with a large scale oscillatory system that acts as a mediary for the perceptual information to reach consciousness-level representation. The relative level of synchronized firing of the neurons representing the features of an object obtained after the second-stage synchronizing modulation is used as the equivalent of conscious perception of the corresponding object. Here, this model is used for simulating interaction of two successive featured objects as a function of stimulus onset asynchrony (SOA). Model output reproduces typical results of mutual masking—with shortest and longest SOAs first and second object correct perception rate is comparable while with intermediate SOAs second object dominates over the first one. Additionally, with shortest SOAs misbinding of features to form illusory objects is simulated by the model.  相似文献   
8.
We compared the upright standing in 7 patients with sensory-motor disorders and 7 healthy subjects (control) before and after 30-s involuntary neck muscle contraction. A trajectory of the center of pressure was recorded during 30-s standing with the eyes open, eyes closed and standing on a foam-rubber with the eyes open. As compared to healthy subjects, patients exhibited an increased body sway area during standing with the eyes open on both the firm surface and foam-rubber and a backward shift of the center of pressure during standing with the eyes both open and closed. Closing the eyes affected the upright standing of patients to a lesser extent than standing of healthy subjects. Involuntary neck muscle contraction within 30 s elicited a backward shift of the center of pressure in healthy subjects, especially during standing with the eyes closed, and a decrease in the length of the center-of-pressure trajectory, especially of its frontal component during standing on the foam-rubber. In patients, a post-effect of the neck muscle contraction manifested itself as a decrease in the body sway area during standing on the foam-rubber and relative increase in the frontal component of the center-of-pressure trajectory during standing with the eyes closed. The results suggest that the upright standing of patients with sensory-motor disorders is more sensitive to somatosensory than visual input, and 30-s neck muscle contraction approach their postural stability to the age-matched control.  相似文献   
9.
The effect of phosphorus addition on survival of Escherichia coli in an experimental drinking water distribution system was investigated. Higher phosphorus concentrations prolonged the survival of culturable E. coli in water and biofilms. Although phosphorus addition did not affect viable but not culturable (VBNC) E. coli in biofilms, these structures could act as a reservoir of VBNC forms of E. coli in drinking water distribution systems.  相似文献   
10.
The aim of this study was to examine experimentally effects of low level, modulated microwaves on human central nervous system function utilizing the phenomenon of visual masking. Ten healthy volunteers, four males and six females, were exposed to electromagnetic field (450 MHz, 0.16 mW/cm2) with 7 Hz modulation frequency. Two photo series (visual stimuli) of unfamiliar, young male faces were presented to the subjects, one picture after another. All the photos were frontal views of unfamiliar faces, which could be recognized only by their unique combinations of features. The task was to identify the pictures from a group of six photos and to decide which order they were presented in. The phenomenon of visual masking is revealed as anamorphosis in subject's perception of two instantaneous visual stimuli presented within a short time interval. When both stimuli were to be recognized correctly and put in the right order, there was a statistically significant difference (P < 0.05) between the identification of the stimulus with microwave electromagnetic field and sham exposure. Recognition of both stimuli in a pair was better under the sham exposure conditions but the actual difference was only 5%. It was concluded that early stages of visual information processing are overwhelmingly robust and routine (and adaptively significant) activities, so that the low level 7 Hz modulated electromagnetic field effects exerted upon it are extremely weak.  相似文献   
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