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Vincent?A. Martinez Rut Besseling Ottavio?A. Croze Julien Tailleur Mathias Reufer Jana Schwarz-Linek Laurence?G. Wilson Martin?A. Bees Wilson?C.K. Poon 《Biophysical journal》2012,103(8):1637-1647
We present a fast, high-throughput method for characterizing the motility of microorganisms in three dimensions based on standard imaging microscopy. Instead of tracking individual cells, we analyze the spatiotemporal fluctuations of the intensity in the sample from time-lapse images and obtain the intermediate scattering function of the system. We demonstrate our method on two different types of microorganisms: the bacterium Escherichia coli (both smooth swimming and wild type) and the biflagellate alga Chlamydomonas reinhardtii. We validate the methodology using computer simulations and particle tracking. From the intermediate scattering function, we are able to extract the swimming speed distribution, fraction of motile cells, and diffusivity for E. coli, and the swimming speed distribution, and amplitude and frequency of the oscillatory dynamics for C. reinhardtii. In both cases, the motility parameters were averaged over ∼ 104 cells and obtained in a few minutes. 相似文献
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Bessou Cécile Tailleur Aurélie Godard Caroline Gac Armelle de la Cour Julie Lebas Boissy Joachim Mischler Pierre Caldeira-Pires Armando Benoist Anthony 《The International Journal of Life Cycle Assessment》2020,25(7):1217-1230
The International Journal of Life Cycle Assessment - Soil organic carbon (SOC) plays a key role in soil functioning and in greenhouse gas exchange with the atmosphere. Land use and land use changes... 相似文献
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