排序方式: 共有75条查询结果,搜索用时 15 毫秒
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Suk Jun Lee Joonbeom Bae Sunhee Kim Seonah Jeong Chang-Yong Choi Sang-Pil Choi Hyun-Sook Kim Woon-Won Jung Jee-Young Imm Sae Hun Kim Taehoon Chun 《Biotechnology letters》2013,35(2):165-173
Treatment of helper T (Th) cells with saponins from soy bean and mung bean prevented their activation by inhibiting cell proliferation and cytokine secretion. However, the saponins did not affect the expression of major histocompatibility complex class II (Ab) and co-stimulatory molecule (CD86) on professional antigen-presenting cells. Instead, the saponins directly inhibited Th cell proliferation by blocking the G1 to S phase cell cycle transition. Moreover, blocking of the cell cycle by the saponins was achieved by decreased expression of cyclin D1 and cyclin E, and constitutive expression of p27KIP1. Saponins also increased stability of p27KIP1 in Th cells after antigenic stimulation. 相似文献
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Jaeyoon Kim Yoon Sup Choi Seyoung Lim Kyungmoo Yea Jong Hyuk Yoon Dong‐Jae Jun Sang Hoon Ha Jung‐Wook Kim Jae Ho Kim Pann‐Ghill Suh Sung Ho Ryu Taehoon G. Lee 《Proteomics》2010,10(3):394-405
Adipogenesis is a complex process that is accompanied by a number of molecular events. In this study, a proteomic approach was adopted to identify secretory factors associated with adipogenesis. A label‐free shotgun proteomic strategy was implemented to analyze proteins secreted by human adipose stromal vascular fraction cells and differentiated adipocytes. A total of 474 proteins were finally identified and classified according to quantitative changes and statistical significances. Briefly, 177 proteins were significantly upregulated during adipogenesis (Class I), whereas 60 proteins were significantly downregulated (Class II). Changes in the expressions of several proteins were confirmed by quantitative RT‐PCR and immunoblotting. One obvious finding based on proteomic data was that the amounts of several extracellular modulators of Wnt and transforming growth factor‐β (TGF‐β) signaling changed during adipogenesis. The expressions of secreted frizzled‐related proteins, dickkopf‐related proteins, and latent TGF‐β‐binding proteins were found to be altered during adipogenesis, which suggests that they participate in the fine regulation of Wnt and TGF‐β signaling. This study provides useful tools and important clues regarding the roles of secretory factors during adipogenic differentiation, and provides information related to obesity and obesity‐related metabolic diseases. 相似文献
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Lee EJ Lee HJ Kamli MR Pokharel S Bhat AR Lee YH Choi BH Chun T Kang SW Lee YS Kim JW Schnabel RD Taylor JF Choi I 《Genomics》2012,100(3):195-202
We report a systematic study of gene expression during myogenesis and transdifferentiation in four bovine muscle tissues and of adipogenesis in three bovine fat tissues using DNA microarray analysis. One hundred hybridizations were performed and 7245 genes of known and unknown function were identified as being differentially expressed. Supervised hierarchical cluster analysis of gene expression patterns revealed the tissue specificity of genes. A close relationship in global gene expression observed for adipocyte-like cells derived from muscle and adipocytes derived from intramuscular fat suggests a common origin for these cells. The role of transthyretin in myogenesis is a novel finding. Different genes were highly induced during the transdifferentiation of myogenic satellite cells and in the adipogenesis of preadipocytes, indicating the involvement of different molecular mechanisms in these processes. Induction of CD36 and FABP4 expression in adipocyte-like cells and adipocytes may share a common pathway. 相似文献
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Yang KJ Shin S Piao L Shin E Li Y Park KA Byun HS Won M Hong J Kweon GR Hur GM Seok JH Chun T Brazil DP Hemmings BA Park J 《The Journal of biological chemistry》2008,283(3):1480-1491
3-Phosphoinositide-dependent protein kinase-1 (PDK1) appears to play a central regulatory role in many cell signalings between phosphoinositide-3 kinase and various intracellular serine/threonine kinases. In resting cells, PDK1 is known to be constitutively active and is further activated by tyrosine phosphorylation (Tyr(9) and Tyr(373/376)) following the treatment of the cell with insulin or pervanadate. However, little is known about the mechanisms for this additional activation of PDK1. Here, we report that the SH2 domain of Src, Crk, and GAP recognized tyrosine-phosphorylated PDK1 in vitro. Destabilization of PDK1 induced by geldanamycin (a Hsp90 inhibitor) was partially blocked in HEK 293 cells expressing PDK1-Y9F. Co-expression of Hsp90 enhanced PDK1-Src complex formation and led to further increased PDK1 activity toward PKB and SGK. Immunohistochemical analysis with anti-phospho-Tyr(9) antibodies showed that the level of Tyr(9) phosphorylation was markedly increased in tumor samples compared with normal. Taken together, these data suggest that phosphorylation of PDK1 on Tyr(9), distinct from Tyr(373/376), is important for PDK1/Src complex formation, leading to PDK1 activation. Furthermore, Tyr(9) phosphorylation is critical for the stabilization of both PDK1 and the PDK1/Src complex via Hsp90-mediated protection of PDK1 degradation. 相似文献
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Jae-Sam Hwang Hyun-Jeong Go Tae-Won Goo Eun-Young Yun Kwang-Ho Choi Su-Il Seong Jong-Su Chang Sang-Mong Lee Bong-Hee Lee Ik-Soo Kim Nam Soon Kim Taehoon Chun Seok-Woo Kang 《Biotechnology letters》2005,26(19):1469-1473
LIM protein cDNA, from Bombyx mori that contains an open reading frame of 622 bp encoding 94 amino acids, was identified and characterized. The B. mori LIM protein homologue is classified into group 2 LIM proteins that contain glycine-rich LIM domain. B. mori LIM protein mRNA is up-regulated at late embryogenesis and detected in the mid-gut of 5th instar larvae. 相似文献
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Stabilization of Insoluble Discharge Products by Facile Aniline Modification for High Performance Li‐S Batteries
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Jae Ho Kim Taehoon Kim Yo Chan Jeong Kunsil Lee Kyung Tae Park Seung Jae Yang Chong Rae Park 《Liver Transplantation》2015,5(14)
Continuous efforts have been made to attain high performance Li‐S batteries by preventing loss of soluble polysulfides, whereas issues related to insoluble discharge products, Li2S2 and Li2S, have been underestimated. A facile and mild method, diazotization, that enables uniform functionalization on the surface of ordered mesoporous carbon (CMK‐3) with aniline functional groups while not deteriorating the original CMK‐3 microstructure is demonstrated. The aniline groups possess favorable interactions with insoluble discharge products. Thus, they homogeneously distribute the insoluble discharge products during cycling. The proposed materials exhibit outstanding electrochemical properties with regard to stability (920 mAh g?1 at 0.2 C after 100 cycles) and rate capability (814 mAh g?1 at 1 C) when evaluated as a cathode material for Li‐S batteries. 相似文献
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Kwon HS Bae YJ Moon KA Lee YS Lee T Lee KY Kim TB Park CS Moon HB Cho YS 《Life sciences》2012,90(13-14):502-508
AimsOxidative stress is involved in the pathogenesis of asthma, and peroxiredoxins (PRDX) may be critical in controlling intracellular oxidative stress. The aim of this study was to evaluate expressions of PRDX and their hyperoxidized forms in asthmatic individuals.Main methodsThe levels of expression of PRDX1, PRDX2, PRDX3, and PRDX6 and their hyperoxidized forms (PRDX-SO3) were measured in PBMCs from asthma patients and control subjects. In addition, cells from these subjects were treated with hydrogen peroxide (H2O2) and their intracellular concentrations of reactive oxygen species (ROS) were measured.Key findingsThe ratios of hyperoxidized to total PRDX (PRDX-SO3/PRDX) in PBMCs were significantly higher in asthma patients than in normal subjects and were correlated with disease severity, with the highest ratio seen in patients with severe asthma. Furthermore, H2O2 treatment of PBMCs, particularly lymphocytes, increased intracellular ROS concentrations with greater and more persistent increases observed in cells from asthmatic than from control subjects.SignificanceHyperoxidation of PRDX may serve as a biomarker of asthma severity and may predict enhanced susceptibility to oxidative stress load in PBMCs of asthmatics. 相似文献
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Kyungmoo Yea Jaeyoon Kim Jong Hyuk Yoon Taewan Kwon Jong Hyun Kim Byoung Dae Lee Hae-Jeong Lee Seung Jae Lee Jong-In Kim Taehoon G. Lee Moon-Chang Baek Ho Seon Park Kyong Soo Park Motoi Ohba Pann-Ghill Suh Sung Ho Ryu 《The Journal of biological chemistry》2009,284(49):33833-33840
Glucose homeostasis is maintained by the orchestration of peripheral glucose utilization and hepatic glucose production, mainly by insulin. In this study, we found by utilizing a combined parallel chromatography mass profiling approach that lysophosphatidylcholine (LPC) regulates glucose levels. LPC was found to stimulate glucose uptake in 3T3-L1 adipocytes dose- and time-dependently, and this activity was found to be sensitive to variations in acyl chain lengths and to polar head group types in LPC. Treatment with LPC resulted in a significant increase in the level of GLUT4 at the plasma membranes of 3T3-L1 adipocytes. Moreover, LPC did not affect IRS-1 and AKT2 phosphorylations, and LPC-induced glucose uptake was not influenced by pretreatment with the PI 3-kinase inhibitor . However, glucose uptake stimulation by LPC was abrogated both by rottlerin (a protein kinase Cδ inhibitor) and by the adenoviral expression of dominant negative protein kinase Cδ. In line with its determined cellular functions, LPC was found to lower blood glucose levels in normal mice. Furthermore, LPC improved blood glucose levels in mouse models of type 1 and 2 diabetes. These results suggest that an understanding of the mode of action of LPC may provide a new perspective of glucose homeostasis. LY294002相似文献