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Fumio Okada Tadakazu Takeo Shoji Okada Osamu Tamemasa 《Bioscience, biotechnology, and biochemistry》2013,77(5):791-794
Theaflavins, the oxidized products formed from tea leaf catechins during black tea fermentation, showed an antiviral activity on TMV. From the survey of the interactions of theaflavins with RNA and its related substances, it was presumed that theaflavins disturbed the replication cycle of TMV through binding to TMV-RNA. 相似文献
3.
Fioretto JR Querioz SS Padovani CR Matsubara LS Okoshi K Matsubara BB 《American journal of physiology. Heart and circulatory physiology》2002,282(4):H1327-H1333
The effects of protein-calorie malnutrition (PCM) on heart structure and function are not completely understood. We studied heart morphometric, functional, and biochemical characteristics in undernourished young Wistar rats. They were submitted to PCM from birth (undernourished group, UG). After 10 wk, left ventricle function was studied using a Langendorff preparation. The results were compared with age-matched rats fed ad libitum (control group, CG). The UG rats achieved 47% of the body weight and 44% of the left ventricular weight (LVW) of the CG. LVW-to-ventricular volume ratio was smaller and myocardial hydroxyproline concentration was higher in the UG. Left ventricular systolic function was not affected by the PCM protocol. The myocardial stiffness constant was greater in the UG, whereas the end-diastolic pressure-volume relationship was not altered. In conclusion, the heart is not spared from the adverse effects of PCM. There is a geometric alteration in the left ventricle with preserved ventricular compliance despite the increased passive myocardial stiffness. The systolic function is preserved. 相似文献
4.
Tetsuro Takayama Susumu Okamoto Tadakazu Hisamatsu Makoto Naganuma Katsuyoshi Matsuoka Shinta Mizuno Rieko Bessho Toshifumi Hibi Takanori Kanai 《PloS one》2015,10(6)
Cytoapheresis (CAP) therapy is widely used in ulcerative colitis (UC) patients with moderate to severe activity in Japan. The aim of this study is to predict the need of operation after CAP therapy of UC patients on an individual level using an artificial neural network system (ANN). Ninety UC patients with moderate to severe activity were treated with CAP. Data on the patients’ demographics, medication, clinical activity index (CAI) and efficacy of CAP were collected. Clinical data were divided into training data group and validation data group and analyzed using ANN to predict individual outcomes. The sensitivity and specificity of predictive expression by ANN were 0.96 and 0.97, respectively. Events of admission, operation, and use of immunomodulator, and efficacy of CAP were significantly correlated to the outcome. Requirement of operation after CAP therapy was successfully predicted by using ANN. This newly established ANN strategy would be used as powerful support of physicians in the clinical practice. 相似文献
5.
Yamamoto K Itoh T Abe D Shimizu M Kanda T Koyama T Nishikawa M Tamai T Ooizumi H Yamada S 《Bioorganic & medicinal chemistry letters》2005,15(3):517-522
We found that putative metabolites of docosahexaenoic acid (DHA) are strong PPARgamma activators and potential antidiabetic agents. We designed DHA derivatives based on the crystal structure of PPARgamma, synthesized them and evaluated their activities in vitro and in vivo. The efficacy of 5E-4-hydroxy-DHA 2a as a PPARgamma activator was about fourfold stronger than that of pioglitazone. Furthermore, the 4-keto derivative (10b) showed antidiabetic activity in animal models without producing undesirable effects such as obesity and hepatotoxicity. 相似文献
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Four fractions with ribonuclease activity have been isolated from tea leaves by DEAE-cellulose column chromatography and designated as RNase Tf-1, RNase Tf-2, RNase Tf-3 and RNase Tf-4. The bigger fractions of both RNase Tf-3 and RNase Tf-4 have been partially purified by Sephadex G-100 column chromatography.RNase Tf-3 and RNase Tf-4 were respectively found to have their optimum pH at 4.75 and 4.9 and molecular weights of approximately 13,000 and 16,000, as determined by gel filtration. Both enzymes were inhibited by Cu2+ and Hg2+, and inactivated by heating at over 50°C. By addition of yeast RNA to the two enzymes, however, their thermostabilities increased. The activities of the enzymes were stable in a pH range of 4.5 to 6.5. Like other plant RNases, RNase Tf-3 and RNase Tf-4 appeared to have no preference for base in RNA. 相似文献
8.
Acid phosphatase in plant tissues I. Changes in activity and multiple forms in tea leaves and tomato fruit during maturation and senescence 总被引:3,自引:0,他引:3
Multiple forms of acid phosphatase varied in tea leaves (Camelliasinensis L.) depending on variety and the age of the leaves.Polyacrylamide gel electrophoresis indicated the presence ofsix multiple forms in extracts of young leaves and seven inextracts of mature leaves of the variety Hatzumomizi. VarietyBenihomare showed five bands in zymograms of young-leaf extractsand six in zymograms of mature-leaf extracts. In addition tothe appearance of the new band, the relative intensity of stainingin two other bands changed during maturation. One decreasedand one increased in intensity. The components were partiallyseparated by CM-cellulose chromatography and isoelectric focusing.Total activity of acid phosphatase did not change greatly ona fresh-weight basis during maturation and aging. Extracts of immature green tomato (Lycopersicon esculentum Mill.)fruit showed six bands on polyacrylamide gels. Isoelectric focusingand subsequent electrophoresis of the fractions obtained atdifferent pH values, produced evidence for seven acid phosphatasecomponents in tomato extracts. Unlike the situation with certainother climacteric fruit, acid phosphatase in tomato extractsdecreased steadily during ripening to a level of activity only2540% that of immature green fruit extracts. It is thereforeconcluded that the onset of senescence in climacteric fruitsis not dependent on an increase in acid phosphatase. Substratespecificity, response to inhibitors, Km, and pH optima of partiallypurified fractions were similar to those of tea-leaf extracts. (Received November 29, 1972; ) 相似文献
9.
Okoshi R Kubo N Nakashima K Shimozato O Nakagawara A Ozaki T 《Biochemical and biophysical research communications》2011,(1):2057-84
Recently, we have described that CREB (cAMP-responsive element-binding protein) has the ability to transactivate tumor suppressor p53 gene in response to glucose deprivation. In this study, we have found that CREB forms a complex with p53 and represses p53-mediated transactivation of MDM2 but not of p21WAF1. Immunoprecipitation analysis revealed that CREB interacts with p53 in response to glucose deprivation. Forced expression of CREB significantly attenuated the up-regulation of the endogenous MDM2 in response to p53. By contrast, the mutant form of CREB lacking DNA-binding domain (CREBΔ) had an undetectable effect on the expression level of the endogenous MDM2. During the glucose deprivation-mediated apoptosis, there existed an inverse relationship between the expression levels of MDM2 and p53/CREB. Additionally, p53/CREB complex was dissociated from MDM2 promoter in response to glucose deprivation. Collectively, our present results suggest that CREB preferentially down-regulates MDM2 and thereby contributing to p53-mediated apoptosis in response to glucose deprivation. 相似文献
10.
Takahide Kakuya Takashi Yamada Masayuki Yokokawa Tadakazu Ueda 《In vitro cellular & developmental biology. Plant》1983,19(8):591-599
Summary We have examined the conditions for cultivation of enzymatically dispersed cells from 34 human urothelial transitional cell
carcinomas (TCC) of various types. By employing two culture methods, stationary and tapping suspension, and by using the synthetic
medium DM 160 supplement with human umbilical cord serum and fetal bovine serum, six cell strains were established. In two
strains the tapping suspension culture method was suitable for growth of highly malignant cancer cells that detach easily
from the glass surface in stationary cultures. Each of the six cell strains has been maintained in culture for over 30 months
with repeated subcultures of 32 to 128 times. The histopathological features of the original TCC were three differentiated
papillary types and three anaplastic nonpapillary types. In two cell strains from TCC with low malignancy, however, the cancer
masses that formed in nude mice differed from the original TCC in which they became more malignant, and one cell strain resembled
the original TCC closely. In three stationary culture cell strains the epithelial nature was demonstrated by the presence
of desmosomes and tonofilaments. In one cell strain only tonofilaments were present. In two tapping suspension culture cell
strains the presence of desmosomes was not shown clearly, but fine tonofilaments were observed in one cell strain.
This work was supported in part by Grants 5319 and 5322 in aid for cancer research from the Ministry of Health and Welfare,
Japan. 相似文献