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TAL KATZ EZOV† SHANG-LIN CHANG†‡ ZE'EV FRENKEL§ AYELLET V. SEGRȶ MORAN BAHALUL REW W. MURRAY¶ JUN-YI LEU‡ ABRAHAM KOROL§ YECHEZKEL KASHI 《Molecular ecology》2010,19(1):121-131
Understanding the evolution of sex and recombination, key factors in the evolution of life, is a major challenge in biology. Studies of reproduction strategies of natural populations are important to complement the theoretical and experimental models. Fungi with both sexual and asexual life cycles are an interesting system for understanding the evolution of sex. In a study of natural populations of yeast Saccharomyces cerevisiae , we found that the isolates are heterothallic, meaning their mating type is stable, while the general belief is that natural S. cerevisiae strains are homothallic (can undergo mating-type switching). Mating-type switching is a gene-conversion process initiated by a site-specific endonuclease HO; this process can be followed by mother–daughter mating. Heterothallic yeast can mate with unrelated haploids (amphimixis), or undergo mating between spores from the same tetrad (intratetrad mating, or automixis), but cannot undergo mother–daughter mating as homothallic yeasts can. Sequence analysis of HO gene in a panel of natural S. cerevisiae isolates revealed multiple mutations. Good correspondence was found in the comparison of population structure characterized using 19 microsatellite markers spread over eight chromosomes and the HO sequence. Experiments that tested whether the mating-type switching pathway upstream and downstream of HO is functional, together with the detected HO mutations, strongly suggest that loss of function of HO is the cause of heterothallism. Furthermore, our results support the hypothesis that clonal reproduction and intratetrad mating may predominate in natural yeast populations, while mother–daughter mating might not be as significant as was considered. 相似文献
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An attempt is made to explain the tendency to excessive wiltingin scabrous diminutive, a pepper mutant. For this, mutant andnormal plants were compared with respect to leaf anatomy, transpirationof whole plants and detached drying leaves, density and openingof stomata, staining of potassium in epidermal cells and rootpressure. A much greater proportion of intracellular space was found inthe mutant leaf which contains fewer and smaller mesophyll cellsthan the normal plant. The anticlinal walls of the epidermisof the mutant leaf were almost straight whereas those of thenormal were wavy. Transpiration per unit leaf area of wholeplants, percentage of stomata open both day and night, and waterloss from detached drying leaves were all higher in the mutant.Potassium staining in guard cells was similar in both planttypes, slightly less in darkness and marginally higher in light.The subsidiary cells of normal leaves did not stain at all,but those of the mutant leaves stained heavily in both lightand darkness. Root pressure was lower in the mutant. Possible explanations for the tendency of the pepper mutantto wilt are discussed. 相似文献
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JAVIER BRUMÓS MANUEL TALÓN RYM BOUHLAL JOSÉ M. COLMENERO‐FLORES 《Plant, cell & environment》2010,33(12):2012-2027
To reveal specific Cl‐ transport activities in the symplastic pathway, uptake, long‐distance transport and distribution of Cl‐ have been investigated in the citrus rootstocks Carrizo citrange (CC, Cl‐ includer) and Cleopatra mandarin (CM, Cl‐ excluder). Using an external concentration of 4.5 mm Cl‐, both species actively transported Cl‐ to levels that exceeded the critical requirement concentration by one and two orders of magnitude in the excluder and the includer rootstocks, respectively. Both CC and CM modulated Cl‐ influx according to the availability of the nutrient as uptake capacity was induced by Cl‐ starvation, but inhibited after Cl‐ resupply. Net Cl‐ uptake was higher in the includer CC, an observation that correlated with a lower root‐to‐shoot transport capacity in the excluder CM. The patterns of tissue Cl‐ accumulation indicated that chloride exclusion in the salt‐tolerant rootstock CM was caused by a reduced net Cl‐ loading into the root xylem. Genes CcCCC1, CcSLAH1 and CcICln1 putatively involved in the regulation of chloride transport were isolated and their expression analysed in response to both changes in the nutritional status of Cl‐ and salt stress. The previously uncharacterized ICln gene exhibited a strong repression to Cl‐ application in the excluder rootstock, suggesting a role in regulating Cl‐ homeostasis in plants. 相似文献
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