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By means of immunohistochemical methods a study of the liver intralobular localization of cytochromes P-450b(+e) and P-450c(+d) has been carried out after separate and consecutive treatment of rats with phenobarbital (PB) and 3-methylcholanthrene (MC). PB-treatment leads to localization of P-450b in the centrilobular region, whereas homogeneous distribution of P-450c in the lobule is observed after MC-treatment. The consecutive treatment with PB and MC is accompanied by the localization of P-450c only in cells of the periportal region of the lobule. PB-treatment of rats after preliminary MC-injection also results in the periportal localization of P-450c, and a small quantity of P-450b is localized in hepatocytes of the centrilobular region. Hence, the consecutive treatment with inducers of different molecular forms of cytochrome P-450 is accompanied by the redistribution of these isoenzymes in parenchymatous cells of the liver lobule. That is confirmed also by biochemical testing and immunochemical analysis of the microsomal fraction of hepatocytes. 相似文献
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The fauna of fleas in the East Kisil-Kum includes 24 species. Different physicogeographic regions of sands are characterized by different spectra and number of species of fleas. The greatest share in collections is composed by Xenopsylla gerbilli caspica--the parasite of Rhombomys opimus. In autumn the other parasite of this rodent, Coptopsylla lamellifer, is a mass species. All the most significant and long changes in the number of these species are associated with changes in the density of their hosts' population. The most significant in epizootic respect is the flea X. gerbilli caspica from which about 50% of plague agent cultures were isolated. 相似文献
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Summary Mutagenic action of 60Co -rays on extracellular phages red
+ and red1 13 after irradiation in 4% nutrient broth in the absence or in the presence of 0.1 M cysteamine or in dried samples was studied. The yield of c mutations was almost independent of the repair genotype of the host cells (uvrA6, polA1, recA13, lexA102, uvrE502, uvrD3 or xthA9), of the phage Red function and of the conditions of -irradiation and was 1·10-12 per base pair and 1 rad. When the SOS-repair system of the host cells was induced by moderate UV irradiation, the yield of c-mutations was drastically enhanced in phage irradiated in broth, but not in phage irradiated in the dried state. These data allow us to suppose that the direct action of -rays induces, in phage DNA, premutational lesions that are fixed into mutations by replication. On the other hand after -irradiation in broth, when indirect radiation effects are only partially suppressed, about 85% of premutational lesions are converted into mutations by means of the inducible, errorprone SOS-repair system. 相似文献
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Oxygen radical-mediated mutagenic effect of asbestos on human lymphocytes: suppression by oxygen radical scavengers. 总被引:5,自引:0,他引:5
L G Korkina A D Durnev T B Suslova Z P Cheremisina N O Daugel-Dauge I B Afanas'ev 《Mutation research》1992,265(2):245-253
The mutagenic effect of chrysotile asbestos fibers and zeolite and latex particles on human lymphocytes in whole blood has been studied. It was concluded that their mutagenic activities were mediated by oxygen radicals because they were inhibited by antioxidant enzymes (SOD and catalase) and oxygen radical scavengers (rutin, ascorbic acid, and bemitil). It was proposed that oxygen radicals were released by phagocytes activated upon exposure to mineral dusts and fibers. The study of lucigenin- and luminol-amplified chemiluminescence of peritoneal macrophages stimulated by chrysotile fibers and zeolite and latex particles has shown that their mutagenic action is probably mediated by different oxygen species, namely, by the iron-oxygen complexes (perferryl ions) plus hydrogen peroxide, hydrogen peroxide, and superoxide ion, respectively. From the oxygen radical scavengers studied, rutin was the most effective inhibitor of the mutagenic effect of mineral fibers and dusts. 相似文献
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Galachyants A. D. Suslova M. Yu. Marinayte I. I. Izosimova O. N. Krasnopeev A. Yu. Shtykova Yu. R. Tikhonova I. V. Podlesnaya G. V. Belykh O. I. 《Microbiology》2020,89(5):609-615
Microbiology - The summer of 2015 was characterized by a large number of forest fires along the coastline of Lake Baikal. The concentrations of all analyzed polycyclic aromatic hydrocarbons (PAHs)... 相似文献
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Sukhanova E. V. Shtykova Yu. R. Suslova M. Yu. Pestunova O. S. Kostornova T. Ya. Khanaev I. V. Zimens E. A. Podlesnaya G. V. Parfenova V. V. 《Microbiology》2019,88(3):324-334
Microbiology - A total of 170 heterotrophic bacterial strains were isolated from Lake Baikal epilithic biofilms. Identification of the isolates was carried out using the 16S rRNA gene sequencing... 相似文献