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Chaowalit Yuajit Sureeporn Homvisasevongsa Lisa Chatsudthipong Sunhapas Soodvilai Chatchai Muanprasat Varanuj Chatsudthipong 《PloS one》2013,8(3)
Cyst enlargement in polycystic kidney disease (PKD) involves cAMP-activated proliferation of cyst-lining epithelial cells and transepithelial fluid secretion into the cyst lumen via cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. This study aimed to investigate an inhibitory effect and detailed mechanisms of steviol and its derivatives on cyst growth using a cyst model in Madin-Darby canine kidney (MDCK) cells. Among 4 steviol-related compounds tested, steviol was found to be the most potent at inhibiting MDCK cyst growth. Steviol inhibition of cyst growth was dose-dependent; steviol (100 microM) reversibly inhibited cyst formation and cyst growth by 72.53.6% and 38.2±8.5%, respectively. Steviol at doses up to 200 microM had no effect on MDCK cell viability, proliferation and apoptosis. However, steviol acutely inhibited forskolin-stimulated apical chloride current in MDCK epithelia, measured with the Ussing chamber technique, in a dose-dependent manner. Prolonged treatment (24 h) with steviol (100 microM) also strongly inhibited forskolin-stimulated apical chloride current, in part by reducing CFTR protein expression in MDCK cells. Interestingly, proteasome inhibitor, MG-132, abolished the effect of steviol on CFTR protein expression. Immunofluorescence studies demonstrated that prolonged treatment (24 h) with steviol (100 microM) markedly reduced CFTR expression at the plasma membrane. Taken together, the data suggest that steviol retards MDCK cyst progression in two ways: first by directly inhibiting CFTR chloride channel activity and second by reducing CFTR expression, in part, by promoting proteasomal degradation of CFTR. Steviol and related compounds therefore represent drug candidates for treatment of polycystic kidney disease. 相似文献
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Sureeporn Khonpikul Napat Jakrawatana Piyapong Sangkaew Shabbir H. Gheewala 《The International Journal of Life Cycle Assessment》2017,22(11):1692-1704
Purpose
The aim of this research is to reveal the overall money flows and physical flows of the livestock and feed production supply chain in Thailand in order to analyze the resource use and cost and assess material use efficiency of the whole supply chain. Another aim is to evaluate the options to improve and evaluate trade-off between economic and environmental performance.Methods
This research conducted material and monetary flow modeling using material flow analysis (MFA) and input output analysis (IOA). Data collected from the Thai Input-Output Tables 2005 were employed to develop the monetary flow model. Direct and indirect resource consumption (energy, water, and land use) and turnover along the supply chain were assessed using environmentally extended input-output analysis model (EEIOA). Scenario analysis with improvement options was applied to the model to evaluate the effectiveness of the improvement options.Results and discussion
One third of energy and water consumption were from the animal farm itself. The rest were from feed production and upstream raw material production. Land use in the system was mainly from maize and paddy field. Feed conversion ratio improvement should play an important role in the strategy for resource efficiency and reduce environmental impact in the whole supply chain. Energy intensity reduction, the best option in overall energy reduction, is the policy that the government is pushing to be implemented in all sectors, and it can also easily be applied along with the other options. Therefore, it should be applied with the other options for improvement.Conclusions
The results from monetary flow and physical material flow can visually show the holistic view of the Thai livestock production supply chain quantitatively and allow the stakeholders to understand the economic structure of the supply chain system. This can enable the decision makers to analyze the interrelation effect and impact of changing one sector demand or changing resource efficiency to impact other sectors in the system.4.
Kereszt A Li D Indrasumunar A Nguyen CD Nontachaiyapoom S Kinkema M Gresshoff PM 《Nature protocols》2007,2(4):948-952
This protocol is used to induce transgenic roots on soybean to study the function of genes required in biological processes of the root. Young seedlings with unfolded cotyledons are infected at the cotyledonary node and/or hypocotyl with Agrobacterium rhizogenes carrying the gene construct to be tested and the infection sites are kept in an environment of high humidity. When the emerged hairy roots can support the plants, the main roots are removed and the transgenic roots can be tested. Using this method, almost 100% of the infected plants form hairy roots within 1 month from the start of the experiments. 相似文献
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Punwong Paramita Promplin Sureeporn Lomchantrasilp Charinee Soonthornampaipong Pornuma Englong Apichaya Marchant Rob Selby Katherine Chirawatkul Prae 《Vegetation History and Archaeobotany》2023,32(1):17-34
Vegetation History and Archaeobotany - Environmental changes and human activities in a mangrove ecosystem in Bang Khun Thian, south of Bangkok, the upper Gulf of Thailand were reconstructed through... 相似文献
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Sequence variation of avirulence gene AVR-Pita1 in rice blast fungus, Magnaporthe oryzae 总被引:1,自引:0,他引:1
Teerapong Kasetsomboon Sureeporn Kate-Ngam Tanee Sriwongchai Bo Zhou Chatchawan Jantasuriyarat 《Mycological Progress》2013,12(4):617-628
The interaction between rice, Oryza sativa, and rice blast fungus, Magnaporthe oryzae, is triggered by an interaction between the protein products of the host resistant gene, and the pathogen avirulence gene. This interaction follows the ‘gene-for-gene' concept. The resistant gene has effectively protected rice plants from rice blast infection. However, the resistant genes usually break down several years after the release of the resistant rice varieties because the fungus has evolved to new races. The objective of this study is to investigate the nucleotide sequence variation of the AVR-Pita1 gene that influences the adaption of rice blast fungus to overcome the resistant gene, Pi-ta. Thirty rice blast fungus isolates were collected in 2005 and 2010 from infected rice plants in northern and northeastern Thailand. The nucleotide sequences of AVR-Pita1 were amplified and analyzed. Phylogenetic analysis was conducted using the MEGA 5.0 program. The results showed a high level of nucleotide sequence polymorphisms and the positive genetic selection pressure in Thai rice blast isolates. The details of sequence variation analysis were described in this article. The information from this study can be used for rice blast resistant breeding program in the future. 相似文献
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Turakulov R Nontachaiyapoom S Mitchelson KR Gresshoff PM Men AE 《Plant & cell physiology》2007,48(9):1379-1384
Detection of very small amounts of RNA based on microdissection of plant tissue is essential for modern plant biology. Mass spectroscopy technology (MassARRAY) based on Sequenomtrade mark instrumentation was adapted to determine quickly and in a high-throughput fashion (by multiplexing) the absolute amounts of mRNA of closely related soybean genes. A sensitivity of 0.1 amol (10(-19)) was achieved, representing as few as 1,000 mRNA molecules. This methodology eliminates the use of housekeeping genes as reference standards and has multiple applications for plant functional genomics, such as the monitoring of individual expression of paralogous genes at ultra-low expression levels and/or in extremely small tissue samples. 相似文献
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