全文获取类型
收费全文 | 1504篇 |
免费 | 99篇 |
国内免费 | 4篇 |
专业分类
1607篇 |
出版年
2023年 | 14篇 |
2022年 | 27篇 |
2021年 | 35篇 |
2020年 | 24篇 |
2019年 | 27篇 |
2018年 | 39篇 |
2017年 | 37篇 |
2016年 | 48篇 |
2015年 | 71篇 |
2014年 | 87篇 |
2013年 | 106篇 |
2012年 | 142篇 |
2011年 | 115篇 |
2010年 | 79篇 |
2009年 | 77篇 |
2008年 | 95篇 |
2007年 | 106篇 |
2006年 | 88篇 |
2005年 | 71篇 |
2004年 | 54篇 |
2003年 | 60篇 |
2002年 | 49篇 |
2001年 | 5篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 8篇 |
1997年 | 7篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 9篇 |
1990年 | 11篇 |
1989年 | 4篇 |
1988年 | 6篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 8篇 |
1979年 | 3篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 8篇 |
1975年 | 6篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 2篇 |
排序方式: 共有1607条查询结果,搜索用时 0 毫秒
1.
2.
Paul J. Chuba Myron A. Leon Amit Banerjee Sunil Palchaudhuri 《Molecular & general genetics : MGG》1989,216(2-3):287-292
Summary
Escherichia coli K12 cells carrying a cloned 1.4 kb HindIII fragment from plasmid ColV2-K94, showed increased survival in guinea pig serum. The recombinant plasmid also conferred group II surface exclusion, i.e. the cells were reduced in recipient ability towards the incoming plasmid R538drd in conjugation experiments. Southern blotting suggested homology with bacteriophage lambda DNA and to the insertion element IS2. Determination of the DNA sequence of the fragment demonstrated the presence of a truncated IS2 (165 bp), separated by 250 bp from a 900 bp stretch of homology with lambda DNA, beginning within the Rz gene and continuing in the rightward direction on the lambda map. A 97 amino acid open reading frame (ORF) adjacent to Rz and on the opposite strand, remained intact in iss, with several amino acid changes. The ORF in iss is preceded by sequences resembling prokaryotic ribosome binding sites and promoters. 相似文献
3.
4.
A restriction enzyme map of the IncFI plasmid ColV2-K94 was generated using EcoRI, BamHI, HindIII, and XhoI; the genetic features of this element were then mapped from previous heteroduplex studies. 相似文献
5.
6.
Isha Gupta Devender Singh Sitender Singh Pawan Kumar Shri Bhagwan Vinod Kumar Harish Kumar Sunil Kumar Chhikara 《Luminescence》2023,38(5):585-599
Terbium(III)-doped yttrium aluminate perovskite (YAP:xTb3+) (x = 0.01–0.08 mol) was synthesized using a simple gel-combustion method. Structural elucidations were performed using X-ray diffraction (XRD) and Rietveld analysis. Fourier-transform infrared spectral studies validated the efficient synthesis of designed doped samples. Transmission electron microscopic images showed the agglomerated irregular dimensions of the synthesized nanocrystalline materials. When excited at 251 nm, a strong emissive line attributed to 5D4 → 7F5 electronic transition was observed at 545 nm (green emission). The maximum luminescence was found at the optimized concentration (0.05 mol) of Tb3+ ions; this emission was quenched by dipolar–dipolar (d–d) interactions. Chromaticity (x and y) and correlated colour temperature parameters were obtained by analysing the emission profiles. Finally, the colour coordinates of nanophosphors were closer to the National Television Standards Committee green coordinates, which replicates their potency in the design and architecture of R-G-B-based white LEDs. 相似文献
7.
8.
Bacillus amyloliquefaciens -amylase activity is pH-dependent and the plot log (Vmax/Km) versus pH implicated a carboxyl group of aspartic acid/glutamic acid at the active site. Chemical modification of -amylase with EDC confirmed this view. Further, analysis of inactivation kinetics showed that modification of a single carboxyl group led to complete loss of the enzymic activity. 相似文献
9.
10.
Isopongaglabol and 6-methoxyisopongaglabol, two new hydroxyfuranoflavones, together with two furanoflavones 5-methoxyfurano(8,7-4″,5″)flavone and 5-methoxy-3′,4′-methylenedioxyfurano(8,7-4″,5″)flavone, two simple flavones, desmethoxykanugin and fisetin tetramethyl ether, a chromenoflavanone, ovalichromene B, two triterpenes, cycloart-23-ene-3β,25-diol and friedelin, and β-sitosterol-β-d-glucoside were isolated from the petrol and CHCl3 extracts of the flowers of Pongamia glabra. The structures of isopongaglabol and 6-methoxyisopongaglabol have been established as 4′-hydroxyfurano(8,7-4″,5″)flavone and 4′-hydroxy-6-methoxyfurano(8,7-4″,5″)flavone, respectively, on the basis of the spectral evidence and they have been confirmed by synthesis. 相似文献