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1.
EA Dukhanina TI Lukyanova EA Romanova V Guerriero NV Gnuchev GP Georgiev DV Yashin LP Sashchenko 《Cell cycle (Georgetown, Tex.)》2015,14(22):3635-3643
PGRP-S (Tag7) is an innate immunity protein involved in the antimicrobial defense systems, both in insects and in mammals. We have previously shown that Tag7 specifically interacts with several proteins, including Hsp70 and the calcium binding protein S100A4 (Mts1), providing a number of novel cellular functions. Here we show that Tag7–Mts1 complex causes chemotactic migration of lymphocytes, with NK cells being a preferred target. Cells of either innate immunity (neutrophils and monocytes) or acquired immunity (CD4+ and CD8+ lymphocytes) can produce this complex, which confirms the close connection between components of the 2 branches of immune response. 相似文献
2.
Biologically active peptide derivatives of 16-member macrolide antibiotics were synthesized as potential probes for the investigation of nascent peptide chain topography in the ribosomal exit tunnel. The tylosin and desmycosin aldehyde groups at the C6 position of the lactone ring were modified by the aminooxyacetyl-L-alanyl-L-alanine methyl ester. 相似文献
3.
Taranenko MV Sumbatyan NV Mtchedlidze MT Kuznetsova SA 《Nucleosides, nucleotides & nucleic acids》2003,22(5-8):1505-1507
New reactive analogs of substrates for DNA repair enzyme E. coli Fpg protein containing the residues of 8-oxoguanine and photoactivatable phenyl(trifluoromethyl)diazirine groups were synthesized. Their substrate properties were investigated. Using photocross-linking technique, we established the presence of contacts of two nucleosides located near the oxoG with amino acids from the Fpg protein. The cross-linking efficiency achieved 10%. 相似文献
4.
A. V. Lokhmatikov N. E. Voskoboynikova D. A. Cherepanov N. V. Sumbatyan G. A. Korshunova M. V. Skulachev H. -J. Steinhoff V. P. Skulachev A. Y. Mulkidjanian 《Biochemistry. Biokhimii?a》2014,79(10):1081-1100
In mammalian mitochondria, cardiolipin molecules are the primary targets of oxidation by reactive oxygen species. The interaction of oxidized cardiolipin molecules with the constituents of the apoptotic cascade may lead to cell death. In the present study, we compared the effects of quinol-containing synthetic and natural amphiphilic antioxidants on cardiolipin peroxidation in a model system (liposomes of bovine cardiolipin). We found that both natural ubiquinol and synthetic antioxidants, even being introduced in micro- and submicromolar concentrations, fully protected the liposomal cardiolipin from peroxidation. The duration of their action, however, varied; it increased with the presence of either methoxy groups of ubiquinol or additional reduced redox groups (in the cases of rhodamine and berberine derivates). The concentration of ubiquinol in the mitochondrial membrane substantially exceeds the concentrations of antioxidants we used and would seem to fully prevent peroxidation of membrane cardiolipin. In fact, this does not happen: cardiolipin in mitochondria is oxidized, and this process can be blocked by amphiphilic cationic antioxidants (Y. N. Antonenko et al. (2008) Biochemistry (Moscow), 73, 1273–1287). We suppose that a fraction of mitochondrial cardiolipin could not be protected by natural ubiquinol; in vivo, peroxidation most likely threatens those cardiolipin molecules that, being bound within complexes of membrane proteins, are inaccessible to the bulky hydrophobic ubiquinol molecules diffusing in the lipid bilayer of the inner mitochondrial membrane. The ability to protect these occluded cardiolipin molecules from peroxidation may explain the beneficial therapeutic action of cationic antioxidants, which accumulate electrophoretically within mitochondria under the action of membrane potential. 相似文献
5.
A. A. Morozova N. V. Sumbatyan V. P. Lezina V. Kh. Akparov G. A. Korshunova T. A. Gudasheva 《Russian Journal of Bioorganic Chemistry》2008,34(5):550-562
Cyclic peptides cyclo(-Gly-Asp-Glu-Lys-), cyclo(-Gly-Gly-Asp-Glu-Lys-) and cyclo(-Gly-Gly-Gly-Asp-Glu-Lys-) were synthesized as models of theβ-turn of nerve growth factor loop 4. The corresponding protected linear precursors were obtained in 52–83% yields by the solid-phase method with the use of the Fmoc/Bu t strategy and a chlorotrityl anchor group. The cyclization was carried out with benzotriazolyloxytris(dimethylamino)phosphonium (BOP) hexafluorophosphate, N-[(1H-benzotriazole-1-yl)-(dimethylamino)methylene]-N-methylmetanaminium-N-oxide (HBTU) hexafluorophosphate, and diphenylphosphorylazide (DPPA) at a dilution of 10?3 M. The distribution of reaction products was studied for each cyclopeptide in dependence on the type of the coupling agent. The use of DPPA was shown to completely inhibit the formation of cyclodimers in the synthesis of five-and six-membered cyclopeptides; however, in the case of a four-membered peptide, an additional tenfold dilution of the reaction mixture was necessary to achieve the effect. The identification of several byproducts during the synthesis showed that the elongation of the polypeptide chain using the BOP reagent can be complicated by substantial racemization, and the cleavage of the chlorotrityl anchor group by 0.5% TFA in dichloromethane proceeds with insufficient selectivity and is accompanied by the premature Boc deblocking of the lysine side function. 相似文献
6.
Antonenko YN Avetisyan AV Cherepanov DA Knorre DA Korshunova GA Markova OV Ojovan SM Perevoshchikova IV Pustovidko AV Rokitskaya TI Severina II Simonyan RA Smirnova EA Sobko AA Sumbatyan NV Severin FF Skulachev VP 《The Journal of biological chemistry》2011,286(20):17831-17840
A limited decrease in mitochondrial membrane potential can be beneficial for cells, especially under some pathological conditions, suggesting that mild uncouplers (protonophores) causing such an effect are promising candidates for therapeutic uses. The great majority of protonophores are weak acids capable of permeating across membranes in their neutral and anionic forms. In the present study, protonophorous activity of a series of derivatives of cationic rhodamine 19, including dodecylrhodamine (C(12)R1) and its conjugate with plastoquinone (SkQR1), was revealed using a variety of assays. Derivatives of rhodamine B, lacking dissociable protons, showed no protonophorous properties. In planar bilayer lipid membranes, separating two compartments differing in pH, diffusion potential of H(+) ions was generated in the presence of C(12)R1 and SkQR1. These compounds induced pH equilibration in liposomes loaded with the pH probe pyranine. C(12)R1 and SkQR1 partially stimulated respiration of rat liver mitochondria in State 4 and decreased their membrane potential. Also, C(12)R1 partially stimulated respiration of yeast cells but, unlike the anionic protonophore FCCP, did not suppress their growth. Loss of function of mitochondrial DNA in yeast (grande-petite transformation) is known to cause a major decrease in the mitochondrial membrane potential. We found that petite yeast cells are relatively more sensitive to the anionic uncouplers than to C(12)R1 compared with grande cells. Together, our data suggest that rhodamine 19-based cationic protonophores are self-limiting; their uncoupling activity is maximal at high membrane potential, but the activity decreases membrane potentials, which causes partial efflux of the uncouplers from mitochondria and, hence, prevents further membrane potential decrease. 相似文献
7.
Galina A. Korshunova Irina A. Ilicheva Natalia V. Sumbatyan Kwang-chul Hyun 《Letters in Peptide Science》1997,4(4-6):473-476
Design and synthesis of oligonucleopeptides (ONPs),structural analogues of oligonucleotides, where the phosphodiester backbone is substituted by a peptidechain, are described. Oligonucleopeptides, in whichthe number of ordinary bonds between the nucleobasesis six and the number of bonds between the backboneand nucleobase is two or four were constructed usingtwo different approaches. The first way is based onincorporation of thyminylalanine residues into the peptidechain alternatively with glycine residues.Experimental studies of the stability ofoligonucleotide–oligonucleopeptide complexes as wellas model estimations of their potential surfacesindicated the low DNA binding efficiency of this typeof reagents. The second approach consists of synthesis of-ornithine peptides followed by modification of thebackbone with thyminylacetaldehyde attached to an-amino function of ornithine residues through Schiffbases. ONPs were synthesized using the solid-phase method. 相似文献
8.
A. G. Tereshchenkov A. V. Shishkina V. N. Tashlitsky G. A. Korshunova A. A. Bogdanov N. V. Sumbatyan 《Biochemistry. Biokhimii?a》2016,81(4):392-400
Chloramphenicol amine peptide derivatives containing tripeptide fragments of regulatory “stop peptides”–MRL, IRA, IWP–were synthesized. The ability of the compounds to form ribosomal complexes was studied by displacement of the fluorescent erythromycin analog from its complex with E. coli ribosomes. It was found that peptide chloramphenicol analogs are able to bind to bacterial ribosomes. The dissociation constants were 4.3-10 μM, which is 100-fold lower than the corresponding values for chloramphenicol amine–ribosome complex. Interaction of the chloramphenicol peptide analogs with ribosomes was simulated by molecular docking, and the most probable contacts of “stop peptide” motifs with the elements of nascent peptide exit tunnel were identified. 相似文献
9.
G. I. Makarov T. M. Makarova N. V. Sumbatyan A. A. Bogdanov 《Biochemistry. Biokhimii?a》2016,81(13):1579-1588
The ribosome as a complex molecular machine undergoes significant conformational changes while synthesizing a protein molecule. Molecular dynamics simulations have been used as complementary approaches to X-ray crystallography and cryoelectron microscopy, as well as biochemical methods, to answer many questions that modern structural methods leave unsolved. In this review, we demonstrate that all-atom modeling of ribosome molecular dynamics is particularly useful in describing the process of tRNA translocation, atomic details of behavior of nascent peptides, antibiotics, and other small molecules in the ribosomal tunnel, and the putative mechanism of allosteric signal transmission to functional sites of the ribosome. 相似文献
10.
Bogdanov AA Sumbatyan NV Shishkina AV Karpenko VV Korshunova GA 《Biochemistry. Biokhimii?a》2010,75(13):1501-1516
This review describes the results of recent studies of the ribosomal tunnel (RT), the major function of which is to allow
the smooth passage of nascent polypeptides with different sequences from the peptidyl transferase center of the ribosome to
the tunnel exit, where the folding of protein molecules begins. The features of structural organization of RT and their role
in modulation and stabilization of the nascent chain conformation are discussed. Structural features of macrolide binding
sites as well as application of macrolide antibiotics and their derivatives as tools to investigate ligand-tunnel wall interactions
are also considered. Several examples of strong and specific interactions of regulatory polypeptides with nucleotide and amino
acid residues of RT that lead to ribosome stalling and translational arrest are described in detail. The role of these events
in regulation of expression of certain genes is discussed on the basis of recent high-resolution structural studies of nascent
chains in the RT. 相似文献