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1.
Hiba Omer Suhaib Alameen Waleed E. Mahmoud Abdelmoneim Sulieman Omaima Nasir Fouad Abolaban 《Saudi Journal of Biological Sciences》2021,28(1):342-346
This study aims to estimate the effective radiation dose and organ dose from head CT procedures. It was conducted in three main private hospitals in Khartoum State-Sudan, using Toshiba machines with 64 slices. The total number of patients included in this study was 142 patients (82 males and 60 females). The effective dose and organ dose were calculated by CT Expo software. The effective dose slightly varied among patients according to gender and age. The effective dose for female patients (5.99 mSv) was higher than that for male patients (5.84 mSv), and the pediatric dose (5.46 mSv) was lower than the adults’ dose (5.94 mSv).The dose for eye lens was found lower for male patients (89.117 mSv) than the dose for female patients (94.62) mSv). According to patients’ age: the dose received by the lens of the eye was much lower in pediatric (79.93 mSv) than the adults (92.41 mSv). The dose for thyroid in female patients (33.52 mSv) was higher than the male patients (28 mSv). The pediatric dose (28.34 mSv) was lower than the adults’ dose (30.64 mSv).Departmental imaging protocol and lack of training among hospital staff are expected to be responsible for these variations. Therefore, this study recommends that the CT technologists be trained on suitable strategies to achieve dose optimization. Moreover, patients’ doses must be monitored regularly. 相似文献
2.
Furdas SD Shekfeh S Bissinger EM Wagner JM Schlimme S Valkov V Hendzel M Jung M Sippl W 《Bioorganic & medicinal chemistry》2011,19(12):3678-3689
We present a combination of database screening, synthesis and in vitro testing to identify novel histone acetyltransferase (HAT) inhibitors. The National Cancer Institute compound collection (NCI) and several commercial databases were filtered by similarity-based virtual screening to find new HAT inhibitors. Employing the recombinant HAT p300/CBP-associated factor (PCAF) and two different histone substrates for screening, pyridoisothiazolones were identified as inhibitors of human PCAF. Due to the limited solubility of the initial hits, we synthesized and tested them on PCAF. The compounds inhibit the proliferation of cancer cells. In summary, valuable chemical tools and potential lead candidates for new anticancer agents directed against HATs as new targets have been identified. 相似文献
3.
Maurer B Mathias U Papatheodorou P Shekfeh S Orth J Jank T Schwan C Sippl W Aktories K Jung M 《Molecular bioSystems》2011,7(3):799-808
ADP-ribosyltransferases (ADP-RTs) use NAD(+) to transfer an ADP-ribosyl group to target proteins. Although some ADP-RTs are bacterial toxins only few inhibitors are known. Here we present the development of fluorescence-based assays and a focussed library screening using kinase inhibitors as a new approach towards inhibitors of ADP-RTs. Different screening setups were established using surrogate small molecule substrates or the quantitation of the cofactor NAD(+). Proof-of-principle screening experiments were performed using a kinase inhibitor library in order to target the NAD(+) binding pockets. This led to the discovery of structurally different lead inhibitors for the mono-ADP-ribosyltransferases Mosquitocidal toxin (MTX) from Bacillus sphaericus SSII-1, C3bot toxin from Clostridium botulinum and CDTa from Clostridium difficile. The interaction of the inhibitors with the toxin proteins was analyzed by means of docking and binding free energy calculations. Binding at the nicotinamide subpocket, which shows a significant difference in the three enzymes, is used to explain the selectivity of the identified inhibitors and offers an opportunity for further development of potent and selective inhibitors. 相似文献
4.
Bo Hu Juan J. Rodriguez Anurag Kakkerla Balaraju Yuanyuan Gao Nhan T. Nguyen Heston Steen Saeb Suhaib Songhai Chen Fang Lin 《The Journal of cell biology》2021,220(12)
Glypicans influence signaling pathways by regulating morphogen trafficking and reception. However, the underlying mechanisms in vertebrates are poorly understood. In zebrafish, Glypican 4 (Gpc4) is required for convergence and extension (C&E) of both the mesoderm and endoderm. Here, we show that transgenic expression of GFP-Gpc4 in the endoderm of gpc4 mutants rescued C&E defects in all germ layers. The rescue of mesoderm was likely mediated by Wnt5b and Wnt11f2 and depended on signaling filopodia rather than on cleavage of the Gpc4 GPI anchor. Gpc4 bound both Wnt5b and Wnt11f2 and regulated formation of the filopodia that transport Wnt5b and Wnt11f2 to neighboring cells. Moreover, this rescue was suppressed by blocking signaling filopodia that extend from endodermal cells. Thus, GFP-Gpc4–labeled protrusions that emanated from endodermal cells transported Wnt5b and Wnt11f2 to other germ layers, rescuing the C&E defects caused by a gpc4 deficiency. Our study reveals a new mechanism that could explain in vivo morphogen distribution involving Gpc4. 相似文献
5.
Dagmara McGuinness Johannes Leierer Olivier Shapter Suhaib Mohammed Marc Gingell-Littlejohn David B. Kingsmore Ann-Margaret Little Julia Kerschbaum Stefan Schneeberger Manuel Maglione Silvio Nadalin Sylvia Wagner Alfred K?nigsrainer Emma Aitken Henry Whalen Marc Clancy Alex McConnachie Christian Koppelstaetter Karen S. Stevenson Paul G. Shiels 《PloS one》2016,11(3)
6.
Ahmed M. Zeyada Khalid A. Al-Gaadi ElKamil Tola Rangaswamy Madugundu Ahmed A. Alameen 《Phyton》2023,92(5):1603-1620
The estuary tides affect groundwater dynamics; these areas are susceptible to waterlogging and salinity issues. A
study was conducted on two fields with a total area of 60 hectares under a center pivot irrigation system that
works with solar energy and belong to a commercial farm located in Northern Sudan. To monitor soil salinity
and calcium carbonate in the area and stop future degradation of soil resources, easy, non-intrusive, and practical
procedures are required. The objective of this study was to use remote sensing-determined Sentinel-2 satellite
imagery using various soil indices to develop prediction models for the estimation of soil electrical conductivity
(EC) and soil calcium carbonate (CaCO3). Geo-referenced soil samples were collected from 72 locations and analyzed in the laboratory for soil EC and CaCO3. The electrical conductivity of the soil saturation paste extract was
represented by average values in soil dataset samples from two fields collected from the topsoil layer (0 to 15 cm)
characteristic of the local salinity gradient. The various soil indices, used in this study, were calculated from the
Sentinel-2 satellite imagery. The prediction was determined using the root mean square error (RMSE) and cross
validation was done using coefficient of determination. The results of regression analysis showed linear relationships with significant correlation between the EC analyzed in laboratory and the salinity index-2 “SI2” (Model-1:
R2 = 0.59, p = 0.00019 and root mean square error (RMSE = 1.32%) and the bare soil index “BSI” (Model-2:
R2 = 0.63, p = 0.00012 and RMSE = 6.42%). Model-1 demonstrated the best model for predicting soil EC, and
validation R2 and RMSE values of 0.48% and 1.32%, respectively. The regression analysis results for soil CaCO3
determination showed linear relationships with data obtained in laboratory and the bare soil index “BSI” (Model-
3: R2 = 0. 45, p = 0.00021 and RMSE = 1.29%) and the bare soil index “BSI” & Normalized difference salinity
index “NDSI” (Model-4: R2 = 0.53, p = 0.00015 and RMSE = 1.55%). The validation confirmed the Model-3 results
for prediction of soil CaCO3 with R2 and RMSE values of 0.478% and 1.29%, respectively. Future soil monitoring
programs might consider the use of remote sensing data for assessing soil salinity and CaCO3 using soil indices
results generated from satellite image (i.e., Sentinel-2). 相似文献
7.
Suhaib M. Siddiqi Xiao-duo Ji Neli Melman Mark E. Olah Rahul Jain Patricia Evans 《Nucleosides, nucleotides & nucleic acids》2013,32(1-3):693-717
Abstract The binding affinities at rat A1, A2a, and A3 adenosine receptors of a wide range of heterocyclic derivatives have been determined. Mono-, bi-, tricyclic and macrocyclic compounds were screened in binding assays, using either [3H]PIA or [3H]CGS 21680 in rat brain membranes or [125I]AB-MECA in CHO cells stably transfected with rat A3 receptors. Several new classes of adenosine antagonists (e. g. 5- oxoimidazopyrimidines and a pyrazoloquinazoline) were identified. Various sulfonylpiperazines, 11- hydroxytetrahydrocarbazolenine, 4H-pyrido[1,2-a]pyrimidin-one, folic acid, and cytochalasin H and J bound to A3 receptors selectively. Moreover, cytochalasin A, which bound to A1 adenosine receptors with Ki value of 1.9 μM, inhibited adenylyl cyclase in rat adipocytes, but not via reversible A1 receptor binding. 相似文献
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9.
Li X McClellan ME Tanito M Garteiser P Towner R Bissig D Berkowitz BA Fliesler SJ Woodruff ML Fain GL Birch DG Khan MS Ash JD Elliott MH 《The Journal of biological chemistry》2012,287(20):16424-16434
Caveolin-1 (Cav-1), an integral component of caveolar membrane domains, is expressed in several retinal cell types, including photoreceptors, retinal vascular endothelial cells, Müller glia, and retinal pigment epithelium (RPE) cells. Recent evidence links Cav-1 to ocular diseases, including autoimmune uveitis, diabetic retinopathy, and primary open angle glaucoma, but its role in normal vision is largely undetermined. In this report, we show that ablation of Cav-1 results in reduced inner and outer retinal function as measured, in vivo, by electroretinography and manganese-enhanced MRI. Somewhat surprisingly, dark current and light sensitivity were normal in individual rods (recorded with suction electrode methods) from Cav-1 knock-out (KO) mice. Although photoreceptor function was largely normal, in vitro, the apparent K(+) affinity of the RPE-expressed α1-Na(+)/K(+)-ATPase was decreased in Cav-1 KO mice. Cav-1 KO retinas also displayed unusually tight adhesion with the RPE, which could be resolved by brief treatment with hyperosmotic medium, suggesting alterations in outer retinal fluid homeostasis. Collectively, these findings demonstrate that reduced retinal function resulting from Cav-1 ablation is not photoreceptor-intrinsic but rather involves impaired subretinal and/or RPE ion/fluid homeostasis. 相似文献
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