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YN Kumar PS Kumar G Sowjenya VK Rao S Yeswanth UV Prasad JA Pradeepkiran P Sarma M Bhaskar 《Bioinformation》2012,8(12):543-547
Hexokinases (HKs) are the enzymes that catalyses the ATP dependent phosphorylation of Hexose sugars to Hexose-6-Phosphate (Hex-6-P). There exist four different forms of HKs namely HK-I, HK-II, HK-III and HK-IV and all of them share a common ATP binding site core surrounded by more variable sequence that determine substrate affinities. Although they share a common binding site but they differ in their kinetic functions, hence the present study is aimed to analyze the binding mode of ATP. The analysis revealed that the four ATP binding domains are showing 13 identical, 7 similar and 6 dissimilar residues with similar structural conformation. Molecular docking of ATP into the kinase domains using Molecular Operating Environment (MOE) soft ware tool clearly showed the variation in the binding mode of ATP with variable docking scores. This probably explains the variable phosphorylation rates among hexokinases family. 相似文献
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Fabrication of metallic nanopillar array structures and their application as electrodes in electrochemical-based biosensors are discussed in this report. Vertically standing nanopillar array structures were fabricated using an electrodeposition technique and their electrochemical characteristics were evaluated. For possible use in biosensing applications, these standing nanopillars should have sufficient mechanical stability to sustain the capillary forces caused by the nanopillar-liquid interactions in aqueous environment and should provide increased signal response in an electrochemical process. Our results showed that the developed nanopillar arrays were mechanically stable in aqueous environments and the nanostructured electrodes exhibited increased electrochemical response compared with flat electrodes. 相似文献
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Uppu Venkateswara Prasad Vimjam Swarupa Sthanikam Yeswanth Pasupuleti Santhosh Kumar Easambadi Siva Kumar Kalikiri Mahesh Kumar Reddy Yellapu Nanda Kumar Vangavaragu Jhansi Rani Abhijit Chaudhary Potukuchi Venkata Gurunadha Krishna Sarma 《Bioinformation》2014,10(2):81-86
Staphylococcus aureus a natural inhabitant of nasopharyngeal tract mainly survives as biofilms and possess complete Krebs cycle
which plays major role in its pathogenesis. This TCA cycle is regulated by Isocitrate dehydrogenase (IDH) we have earlier cloned,
sequenced (), expressed and characterized this enzyme from S. aureus ATCC12600. We have observed only one type of
IDH in all the strains of S. aureus which dictates the flow of carbon thereby controlling the virulence and biofilm formation, this
phenomenon is variable among bacteria. Therefore in the present study comparative structural and functional analysis of IDH was
undertaken. As the crystal structure of S. aureus IDH was not available therefore using the deduced amino sequence of complete
gene the 3D structure of IDH was built in Modeller 9v8. The PROCHECK and ProSAweb analysis showed the built structure was
close to the crystal structure of Bacillus subtilis. This structure when superimposed with other bacterial IDH structures exhibited
extensive structural variations as evidenced from the RMSD values correlating with extensive sequential variations. Only 24%
sequence identity was observed with both human NADP dependent IDHs (PDB: 1T09 and 1T0L) and the structural comparative
studies indicated extensive structural variations with an RMSD values of 14.284Å and 10.073Å respectively. Docking of isocitrate to
both human IDHs and S. aureus IDH structures showed docking scores of -11.6169 and -10.973 respectively clearly indicating
higher binding affinity of isocitrate to human IDH. HM067707相似文献
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Venkataramani Anandan Xiaoling Yang Euihyeon Kim Yeswanth L Rao Guigen Zhang 《Journal of biological engineering》2007,1(1):5-10
The use of nanopillar array electrodes (NAEs) for biosensor applications was explored using a combined experimental and simulation
approach to characterize the role of reaction kinetics and mass transport in glucose detection with NAEs. Thin gold electrodes
with arrays of vertically standing gold nanopillars were fabricated and their amperometric current responses were measured
under bare and functionalized conditions. Results show that the sensing performances of both the bare and functionalized NAEs
were affected not only by the presence and variation of the nanoscale structures on the electrodes but also by the reaction
kinetics and mass transport of the analyte species involved. These results will shed new light for enhancing the performance
of nanostructure based biosensors. 相似文献
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Hanumanthu Prasanna Lakshmi Sthanikam Yeswanth Uppu Venkateswara Prasad Dudipeta Vasu Vimjam Swarupa Pasupuleti Santhosh Kumar Mangamoori Lakshmi Narasu Potukuchi Venkata Gurunadha Krishna Sarma 《Bioinformation》2013,9(4):169-173
Glucose-6-phosphate (G-6-P) formation in Staphylococcus aureus is catalysed by glucokinase (glkA) gene under high glucose
concentration leading to upregulation of various pathogenic factors; therefore the present study is aimed in the cloning and
characterization of glk A gene from S. aureus ATCC12600. The glk A gene was cloned in the Sma I site of pQE 30, sequenced
(Accession number: ) and expressed in E. coli DH5α. The recombinant glk A expressed from the resultant glk A 1 clone
was purified using nickel metal chelate chromatography, the pure enzyme gave single band in SDS-PAGE with molecular weight
of 33kDa. The rglk A showed very high affinity to glucose Km 5.1±0.06mM with Hill coefficient of 1.66±0.032mM. Analysis of
glucokinase sequence of S. aureus showed presence of typical ATP binding site and ROK motif CNCGRSGCIE. Sequentially and
phylogenetically S. aureus glk A exhibited low identity with other bacterial glk A and 21% homology with human glucokinase
(GCK). Functionally, S. aureus glk A showed higher rate of G-6-P formation compared to human GCK which may have profound
role in the pathogenesis. JN645812相似文献
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