排序方式: 共有35条查询结果,搜索用时 15 毫秒
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Kovalenko MV Stepchenko LM Kulinich AO Shevtsova AI 《Ukrainski? biokhimicheski? zhurnal》2010,82(6):58-64
The paper presents new information about the carbohydrate structures of 39-days chicken's fibronectin. It is found out that chicken fibronectin contains mainly biantennary N-glycans with a core fucose and fucosylated O-glycans. It is shown that N-glycans of chicken fibronectin are poorly sialated, since this protein exhibits affinity for the PNA and weak binding to sialospecific SNA. A comparative analysis of lectin-binding activity of chicken and human fibronectins has shown that both glycoproteins differ in glycan composition. 相似文献
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A G Stepchenko N N Luchina F Franek O L Polianovski? 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1988,(10):20-23
The nuclear extracts of plasmacytomas producing antibodies were found to contain factors which formed complexes with the promoter fragment of the gene for immunoglobulin kappa-chains. The corresponding complexes found in the extracts of nonlymphoid cells had a different mobility. Two approaches were proposed for determining the boundaries of the region necessary for protein factors to be bound to DNA using nuclease Ba131. A 5'-ATTTGCAT-3' octanucleotide sequence was shown to be necessary for interaction with the protein nuclear factor in the studied plasmacytoma lines. The protein completely lost its affinity if at least one nucleotide was removed or substituted at the 5'- or 3'-end of this sequence. The procedures proposed for determining the precise boundaries of the sequence necessary for protein binding to DNA do not require a preliminary protein purification. The principles on which the procedures are based, set no limitations to their application to other systems used for studying the interaction of proteins with DNA. 相似文献
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The effect of sodium humate on metabolism and resistance in highly productive poultry] 总被引:1,自引:0,他引:1
L M Stepchenko L V Zhorina L V Kravtsova 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1991,(10):90-95
Natrium humate introduced into broiler chickens Broiler-6 line increases mass on 5-7% on the average and poultry safety on 3-5%. In chicken tissues the indexes of protein, mineral and lipid metabolism normalize, the organism's protection and adaptation opportunities improve as a result of peat preparation influence on fodder component mastering and regulatory processes. 相似文献
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I M Stepchenko V A Berezin A D Reva A I Ialovo? 《Biulleten' eksperimental'no? biologii i meditsiny》1975,80(10):43-46
Molecular forms of cathepsin D bound with subcellular structures were studied in the grey matter of the large hemispheres. Free and bound forms of the enzymes exposed to solubilization with detergent triton X-100 were fractionated by passage through a Sephadex G-100 column. Gel chromatographic analysis demonstrated three peaks of acid proteinase activity. Different areas of solubilization curves of acid proteinases corresponded to different molecular forms of cathepsins. The initial S-shape areas of solubilization curve corresponded to the first high molecular weight peak of the enzyme activity in the grey matter, whereas the subsequent linear ones -- to the second peak; the activity of free forms of the enzyme corresponded to the third peak. 相似文献