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A L Liozner E N Stepanova N A Volynskaia G K Vorkunova A G Bukrinskaia 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(3):91-96
A test system permitting the detection of influenza virus protein M at a concentration of 0.1-0.5 ng/ml in ELISA has been developed. The use of this system made it possible to detect influenza viruses A and B directly in crude virus-containing material and clinical samples obtained from influenza patients. During the outbreak of influenza in the spring of 1983 ELISA was successfully used for the rapid diagnosis of influenza, and some of its advantages in comparison with the conventional immunofluorescence test were thus demonstrated. To overcome difficulties arising from the low immunogenic potency of protein M, in the process of obtaining diagnostic sera and ascitic fluids the animals were immunized with the conjugate of protein M and polyelectrolite, which ensured considerable activation of humoral immune response. 相似文献
3.
A mathematical model of autoimmunity is developed. This model is a system of two nonlinear differential equations, which describe the concentration dynamics of tissue cells and agressive lymphocytes. An analysis of the solutions shows that this model reproduces general behaviour of autoimmune diseases. 相似文献
4.
S V Prozorovski? F M Kirillova L K Stepanova A L Gorelov G A Levina 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(4):24-26
The antigenic structure of the L-forms of salmonellae (S. typhimurium and S. typhi) in comparison with that of the initial bacterial cultures and revertant cultures was studied with the use of the immunoferritin method. The L-forms of salmonellae were found to retain an insignificant amount of O-antigen, as well as to have K-antigen on the surface of the cytoplasmic membrane, but in a lesser amount than the initial strains. In the cultures reverting from the L-forms of S. typhimurium and S. typhi the quantitative and qualitative characteristics of O- and K-antigens were completely restored. 相似文献
5.
The survival rate of Mycobacterium lacticolum and the proportion between its R, S and M variants were studied in the course of 12 months under different conditions of storage (freeze-drying, under vaseline oil, in 0.85% NaCl solution and in distilled water). A high survival rate of the variants was found in cells freeze-dried in a protective medium containing 10% of sucrose +1% of gelatin as well as in a 0.85% solution of NaCl.The survival rate of te variants differed by 2--3 orders of magnitude in cells freeze-dried with sodium glutamate or suspended in distilled water. The proportion between the R, S and M variants in the population noticeable changed after storage under these conditions. 相似文献
6.
Effect of Soviet bleomycin-bleomycetin on the body of animals in multiple parenteral administration]
L E Gol'dberg N G Shepelevtseva T P Vertogradova E S Stepanova V S Murave?skaia 《Antibiotiki》1979,24(11):847-853
Toxicity of bleomycetin was studied on 3 animal species (rats, rabbits and dogs). The antibiotic was administered intramuscularly and intravenously in various doses for a prolonged period of time. The death of the rats, rabbits and dogs treated with repeated lethal doses of bleomycetin was due to its toxic effect on the kidneys and probably lungs. The level of urea in the blood of the animals before death increased up to 300--400 mg %. Histological examination of the kidneys revealed the picture of glomerulonephritis. The lungs were highly plethoric and showed areas of alveolar collapse and consolidation consisting mainly of the collapsed alveolar epithelium. The liver was not affected by bleomycetin according to both the results of some functional tests and histological examination. tthe blood sugar level after bleomycetin administration was not altered significantly. The changes in the peripheral blood were not pronounced. An increased P wave, decreased R wave and deep S wave were seen on the ECG. Such deviitions may be due not only to the changes in the myocardium but also to the lung affection. When bleomycetiin was used repeatedly in nonlethal doses (1 mg/kg for rats, 1--2 mg/kg for rabbits and 0.25--0.5 mg/kg for dogs), the above changes were less pronounced or not manifested at all. No inhibitory effect on hemopoiesis is an important positive characteristics of bleomycetin, so that it compares very favourably with most other antitumor drugs. 相似文献
7.
D S Gordon V A Bochkarev G N Stepanova 《Arkhiv anatomii, gistologii i émbriologii》1991,101(9-10):81-84
Contents of histamine, 5-hydroxytryptamine, functional state of heparinic proteoglycan have been studied in the rat peritoneal mast cells during various seasons of the year (January-February, May-June, July). In winter the mast cells have a high content of histamine and 5-hydroxytryptamine, heparinic proteoglycan of their granules is stained with both alcian blue and safranin. In summer (July) content of histamine in the mast cells is sharply decreased in comparison with that of 5-hydroxytryptamine and in May-June the content of both amines is decreased nearly to background values. Both during spring and summer periods heparinic proteoglycan of the mast cell granules is stained only with alcian blue and does not take safranin. A suggestion is made on independence of the seasonal changes of annual rhythmical pattern of histamine and 5-hydroxytryptamine contents in the mast cells. A conclusion is made concerning possible participation of the mast cell system of organs and tissues in the seasonal changes of biogenic amine levels in them. 相似文献
8.
The methods for chimeric chromosomes' destabilization and the standard tetrad analysis have been used for mapping of the yeast genes XRS2 and HIM1. The genes are localized in the right arm of chromosome IV in the following order: XRS2-31cM-ADE8-28.5cM-HIM1. 相似文献
9.
A. A. Stepanova Yu. V. Lyupina N. P. Sharova P. A. Erokhov 《Doklady. Biochemistry and biophysics》2016,468(1):200-202
Native structure of active forms of rat liver immune proteasomes has been studied by two-dimensional electrophoresis method modified for analysis of unpurified protein fractions. The developed method allowed revealing the proteasome immune subunits LMP7 and LMP2 in 20S subparticles and in the structures bound to one or two PA28αβ activators, but not to the PA700 activator, which is involved in the hydrolysis of ubiquitinated proteins. The results obtained indicate the participation of the immune proteasomes in delicate regulatory mechanisms based on the production of biologically active peptides and exclude their participation in processes of crude degradation of “rotated” ubiquitinated proteins. 相似文献
10.
S. E. Bresler L. A. Noskin O. K. Kaboev I. M. Stepanova E. F. Davidenkova O. A. Rosenberg E. I. Schwartz A. G. Beketova A. T. Akhmedov 《Molecular & general genetics : MGG》1981,181(4):532-534
Summary Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons). 相似文献