全文获取类型
收费全文 | 265篇 |
免费 | 38篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2017年 | 9篇 |
2016年 | 5篇 |
2015年 | 11篇 |
2014年 | 13篇 |
2013年 | 15篇 |
2012年 | 10篇 |
2011年 | 9篇 |
2010年 | 14篇 |
2009年 | 15篇 |
2008年 | 3篇 |
2007年 | 7篇 |
2006年 | 8篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2003年 | 10篇 |
2002年 | 7篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 10篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 6篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 8篇 |
1986年 | 6篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 7篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 4篇 |
1974年 | 5篇 |
1967年 | 2篇 |
1964年 | 1篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有303条查询结果,搜索用时 15 毫秒
1.
V. Molitor M. Trnka W. Erber I. Steffan M. -E. Rivière B. Arrio H. Springer-Lederer G. A. Peschek 《Archives of microbiology》1990,154(2):112-119
During adaptation of photoautotrophically growing fresh water cyanobacterium Anacystis nidulans to high salinity the cells showed a pronounced increase of proton-sodium antiporter activity, and of cytochrome c oxidase in isolated and purified plasma membrane. At the same time the concentrations of plasma membrane-bound EDTA-resistant copper and iron (determined by inductively coupled plasma atomic emission spectrometry) rose proportionately, accompanied by an increase in whole cell respiration. In plasma membranes from salt adapted cells lipid/protein ratios were markedly higher than in control cells, levels of esterified saturated and long-chain fatty acids being significantly higher than the respective levels of unsaturated and short-chain fatty acids which explains the higher lipid-phase transition temperatures derived from Arrhenius plots. Immunoblotting of the membrane proteins with antisera raised against the cytochrome c oxidases from Paracoccus denitrificans and A. nidulans gave two cross-reacting bands with apparent molecular weights around 50000 and 30000 (subunits I and II, respectively) which were more pronounced in plasma membranes from salt adapted cells when compared to control cells. The protein pattern of plasma membranes from salt adapted cells also showed the appearance of bands at apparent molecular weights of 44000–48000 and 54000–56000 which might stem from the proton/sodium-antiporter in this membrane.Abbreviations CM
cytoplasmic or plasma membrane
- ICM
intracytoplasmic or thylakoid membrane
- cyt
cytochrome
- DCCD
N,N-dicyclohexylcarbodiimide
- Hepes
N-2-hydroxyethylpiperazine-N-2-ethanesulfonate
- ICP-AES
inductively coupled plasma atomic emission spectrometry
- SDS-PAGE
sodium dodecylsulfate polyacrylamide gel electrophoresis
- EPR
electron paramagnetic resonance spectrometry 相似文献
2.
Effects of acidification on mercury methylation, demethylation, and volatilization in sediments from an acid-susceptible lake 总被引:1,自引:0,他引:1
The effect of experimental acidification on mercury methylation, demethylation, and volatilization was examined in surficial sediment samples from a weakly buffered northern Wisconsin lake. All mercury transformations were measured with radioisotopic tracers. Acidification of sediment pH with H2SO4, HCl, or HNO3 significantly decreased 203Hg(II) methylation. Acidification of pH 6.1 (ambient) sediments to pH 4.5 with either H2SO4 or HCl inhibited methylation by over 65%. The decreased methylation was due to the increased hydrogen ion concentration because methylation was not affected by concentrations of Na2SO4 or NaCl equimolar to the amount of acid added. Inhibition of methylation was observed even after prolonged acidification of sediments to pH 5.0 for up to 74 days. Acidification of sediments to pH 5.5, 4.5, and 3.5 with HNO3 resulted in a near complete inhibition of methylation at each pH. Similarly, the addition of equimolar amounts of NaNO3 resulted in a near complete inhibition of methylation, indicating that the inhibition was due to the nitrate ion rather than to the acidity. Demethylation of methyl mercury was not affected by pHs between 8.0 and 4.4, but sharply decreased below pH 4.4. Volatilization of 203Hg(II) from surface sediments was less than 2% of methylation activity and was not significantly different from that in killed sediments. This study indicated that acidification of sediments inhibits mercury methylation and that the observed increase in the mercury burdens in fish from low pH lakes is not due to increased production of methylmercury in sediments. 相似文献
3.
4.
5.
6.
7.
8.
9.
10.
C Crone J Frokjaer-Jensen JJ Friedman O Christensen 《The Journal of general physiology》1978,71(2):195-220