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排序方式: 共有97条查询结果,搜索用时 15 毫秒
1.
A. C. Steere R. L. Grodzicki J. E. Craft M. Shrestha A. N. Kornblatt S. E. Malawista 《The Yale journal of biology and medicine》1984,57(4):557-560
Since the summer of 1982, we have cultured patient specimens for Lyme disease spirochetes. Of 118 patients cultured, four specimens yielded spirochetes: two from blood, one from a skin biopsy specimen of erythema chronicum migrans (ECM), and one from cerebrospinal fluid. All four isolates appeared identical when examined with a monoclonal antibody. However, attempts to recover the spirochete from synovium or synovial fluid were unsuccessful. In addition, the organism could not be visualized in skin or synovial biopsy specimens using the avidin-biotin peroxidase complex detection system. Thus, the current yield in culturing spirochetes from patients is quite low, and it is not yet known whether the organism is still alive later in the disease when arthritis is present. 相似文献
2.
The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation 总被引:48,自引:38,他引:10
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The structure and function of the centrosomes from Chinese hamster ovary (CHO) cells were investigated by electron microscopy of negatively stained wholemount preparations of cell lysates. Cells were trypsinized from culture dishes, lysed with Triton X-100, sedimented onto ionized, carbon-coated grids, and negatively stained with phosphotungstate. The centrosomes from both interphase and dividing cells consisted of pairs of centrioles, a fibrous pericentriolar material, and a group of virus-like particles which were characteristic of the CHO cells and which served as markers for the pericentriolar material. Interphase centrosomes anchored up to two dozen microtubules when cells were lysed under conditions which preserved native microtubules. When Colcemid-blocked mitotic cells, initially devoid of microtubules, were allowed to recover for 10 min, microtubules formed at the pericentriolar material, but not at the centrioles. When lysates of Colcemid-blocked cells were incubated in vitro with micotubule protein purified from porcine brain tissue, up to 250 microtubules assembled at the centrosomes, similar to the number of microtubules that would normally form at the centrosome during cell division. A few microtubules could also be assembled in vitro onto the ends of isolated centrioles from which the pericentriolar material had been removed, forming characteristic axoneme- like bundles. In addition, microtubules; were assembled onto fragments of densely staining, fibrous material which was tentatively identified as periocentriolar material by its association of CHO can initiate and anchor microtubules both in vivo and in vitro. 相似文献
3.
William Campbell Steere 《Brittonia》1982,34(4):435-441
Eobruchia ecuatoriana sp. nov. (Bruchiaceae) has the aspect of a diminutiveTrematodon, but is distinctive in having a mitrate calyptra and flexuous seta;Macromitrium perreflexum sp. nov. (Orthotrichaceae) has ramarkably reflexed leaves, unlike any known species;Lepidopilum pulcherrimum sp. nov. (Hookeriaceae) is distinguished by its large size and beautifully undulate leaves;Symphyodon americanus sp. nov. (Symphyodontaceae) is remarkable in being the first American representative of a genus and family previously known only from Asia; it is most closely related toS. echinatus (Mitt.) Jaeg. of the Himalayas and Thailand. 相似文献
4.
One hundred and forty-five taxa of bryophytes are reported from the extreme southeastern Yukon. Eight species of Hepaticae and 13 of mosses represent new records for the Yukon, which are Calypogeia suecica (S. Arnell & Perss.) K. Müll., Chiloscyphus pallescens (Ehrh.) Dum., Conocephalum conicum (L.) Dum., Jamesoniella autumnalis (DC.) Steph., Pellia neesiana (Gott.) Limpr., Ptilidium pulcherrimum (Web.) Hampe, Riccardia palmata (Hedw.) Carruth., Tritomaria exsecta (Schmid.) Schiffn., Amblystegium varium (Hedw.) Lindb., Brachythecium rivulare B.S.G., B. rutabulum (Hedw.) B.S.G., Bryum blindii B.S.G., Didymodon rigidulus Hedw., D. tophaceus (Brid.) Lisa, Drepanocladus trichophyllus (Warnst.) Podp., Hygroamblystegium noterophilum (Sull. & Lesq. ex Sull.) Warnst., Hygrohypnum molle (Hedw.) Loeske, Plagiomnium ciliare (C. Müll.) Kop., P. cuspidatum (Hedw.) Kop., Platydictya minutissimum (Sull. & Lesq. ex Sull.) Crum, and Pylaisiella selwynii (Kindb.) Crum, Steere & Anderson. Many of the other collections represent wide extensions of range within the Yukon Territory. 相似文献
5.
Robert B. Lochhead David Ordoez Sheila L. Arvikar John M. Aversa Luke S. Oh Benton Heyworth Ruslan Sadreyev Allen C. Steere Klemen Strle 《Cellular microbiology》2019,21(2)
Lyme arthritis (LA), a late disease manifestation of Borrelia burgdorferi infection, usually resolves with antibiotic therapy. However, some patients develop proliferative synovitis lasting months to several years after spirochetal killing, called postinfectious LA. In this study, we phenotyped haematopoietic and stromal cell populations in the synovial lesion ex vivo and used these findings to generate an in vitro model of LA using patient‐derived fibroblast‐like synoviocytes (FLS). Ex vivo analysis of synovial tissue revealed high abundance of IFNγ‐producing T cells and NK cells. Similar to marked IFNγ responses in tissue, postinfectious LA synovial fluid also had high levels of IFNγ. HLA‐DR‐positive FLS were present throughout the synovial lesion, particularly in areas of inflammation. FLS stimulated in vitro with B. burgdorferi, which were similar to conditions during infection, expressed 68 genes associated primarily with innate immune activation and neutrophil recruitment. In contrast, FLS stimulated with IFNγ, which were similar to conditions in the postinfectious phase, expressed >2,000 genes associated with pathogen sensing, inflammation, and MHC Class II antigen presentation, similar to the expression profile in postinfectious synovial tissue. Furthermore, costimulation of FLS with B. burgdorferi and IFNγ induced greater expression of IL‐6 and other innate immune response proteins and genes than with IFNγ stimulation alone. These results suggest that B. burgdorferi infection, in combination with IFNγ, initiates the differentiation of FLS into a highly inflammatory phenotype. We hypothesise that overexpression of IFNγ by lymphocytes within synovia perpetuates these responses in the postinfectious period, causing proliferative synovitis and stalling appropriate repair of damaged tissue. 相似文献
6.
Trollmo C Meyer AL Steere AC Hafler DA Huber BT 《Journal of immunology (Baltimore, Md. : 1950)》2001,166(8):5286-5291
Antibiotic treatment-resistant Lyme arthritis is a chronic inflammatory joint disease that follows infection with Borrelia burgdorferi (BB:). A marked Ab and T cell response to BB: outer surface protein A (OspA) often develops during prolonged episodes of arthritis. Furthermore, cross-reaction between the bacterial OspA and human LFA-1alpha(L) at the T cell level and the inability to detect BB: in the joint implicate an autoimmune mechanism. To analyze the nature of response to OspA and LFA-1alpha(L), we used OspA-specific T cell hybrids from DR4 transgenic mice, as well as cloned human cells specific for OspA(165-184), the immunodominant epitope, from five DRB1*0401(+) patients, using OspA-MHC class II tetramers. Although OspA(165-184) stimulated nearly all OspA-specific human T cell clones tested to proliferate and secrete IFN-gamma and IL-13, LFA-1alpha(L326-345) stimulated approximately 10% of these clones to proliferate and a greater percentage to secrete IL-13. Assays with LFA- or OspA-DR4 monomers revealed that higher concentrations of LFA-DR4 were needed to stimulate dual-reactive T cell hybrids. Our analysis at the clonal level demonstrates that human LFA-1alpha(L326-345) behaves as a partial agonist, perhaps playing a role in perpetuating symptoms of arthritis. 相似文献
7.
Synthesis and biological activity of novel S-adenosyl-L-homocysteine hydrolase inhibitors 总被引:1,自引:0,他引:1
Four potential S-adenosyl-L-homocysteine hydrolase inhibitors were prepared and tested against purified recombinant rat liver enzyme. Preliminary studies indicate that three of these compounds, 1, 2, and 4, caused time-dependent inactivation of S-adenosyl-L-homocysteine hydrolase but showed a biphasic nature. Compound 3 was found to be a rapid equilibrium inhibitor of this enzyme. 相似文献
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10.
William A Cafruny Richard G Duman Grace HW Wong Suleman Said Pam Ward-Demo Raymond RR Rowland Eric A Nelson 《Virology journal》2006,3(1):1-17