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1.
Pradeep Kumar Papolu Nagavara Prasad Gantasala Divya Kamaraju Prakash Banakar Rohini Sreevathsa Uma Rao 《PloS one》2013,8(11)
Root knot nematode, Meloidogyne incognita, is an obligate sedentary endoparasite that infects a large number of crop species and causes substantial yield losses. Non-chemical based control strategies for these nematodes are gaining importance. In the present study, we have demonstrated the significance of two FMRFamide like peptide genes (flp-14 and flp-18) for infection and development of resistance to M. incognita through host-derived RNAi. The study demonstrated both in vitro and in planta validation of RNAi-induced silencing of the two genes cloned from J2 stage of M. incognita. In vitro silencing of both the genes interfered with nematode migration towards the host roots and subsequent invasion into the roots. Transgenic tobacco lines were developed with RNAi constructs of flp-14 and flp-18 and evaluated against M. incognita. The transformed plants did not show any visible phenotypic variations suggesting the absence of any off-target effects. Bioefficacy studies with deliberate challenging of M. incognita resulted in 50-80% reduction in infection and multiplication confirming the silencing effect. We have provided evidence for in vitro and in planta silencing of the genes by expression analysis using qRT-PCR. Thus the identified genes and the strategy can be used as a potential tool for the control of M. incognita. This is the first ever report that has revealed the utility of host delivered RNAi of flps to control M. incognita. The strategy can also be extended to other crops and nematodes. 相似文献
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Sowmya H. Reddy Sumanth K. Kambalimath Rajesh K. Singhal Manjunath K. Chikkakariyappa Raveendran Muthurajan Mavinahalli P. Rajanna Rohini Sreevathsa Amitha M. Sevanthi Trilochan Mohapatra Neelamraju Sarla Viswanathan Chinnusamy Gopala S. Krishnan Ashok K. Singh Nagendra K. Singh Rameshwar P. Sharma Sreeman M. Sheshshayee 《Physiologia plantarum》2019,166(2):596-611
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M. Manjulatha Rohini Sreevathsa A. Manoj Kumar Chinta Sudhakar T. G. Prasad Narendra Tuteja M. Udayakumar 《Molecular biotechnology》2014,56(2):111-125
Peanut, a major edible oil seed crop globally is predominantly grown under rainfed conditions and suffers yield losses due to drought. Development of drought-tolerant varieties through transgenic technology is a valid approach. Besides superior water relation traits like water mining, intrinsic cellular level tolerance mechanisms are important to sustain the growth under stress. To achieve this objective, the focus of this study was to pyramid drought adaptive traits by overexpressing a stress responsive helicase, PDH45 in the background of a genotype with superior water relations. PCR, Southern, and RT-PCR analyses confirmed stable integration and expression of the PDH45 gene in peanut transgenics. At the end of T3 generation, eight transgenic events were identified as promising based on stress tolerance and improvement in productivity. Several transgenic lines showed stay-green phenotype and increased chlorophyll stability under stress and reduced chlorophyll retardation under etherel-induced simulated stress conditions. Stress-induced root growth was also substantially higher in the case of transformants. This was reflected in increased WUE (low Δ13C) and improved growth rates and productivity. The transgenics showed 17.2 and 26.75 % increase in yield under non-stress and stress conditions over wild type ascertaining the feasibility of trait pyramiding strategy for the development of drought-tolerant peanut. 相似文献
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Nagaveni Venkataramaiah Sashidhar Vudayagiri Ramakrishna Rohini Sreevathsa 《Biologia》2011,66(6):1060-1073
Phytoremediation is an important strategy adapted by plants to sequester and/or detoxify pollutants. Phytochelatins, a family
of cysteine-rich thiol-reactive peptides, bind to various heavy metals and metalloids making them good candidates for phytoremediation.
Phytochelatin synthase catalyses the final step in the biosynthesis of phytochelatins and can be used as a strategy to improve
tolerance against heavy metals. In the present study, an AtPCS gene was overexpressed in rice following the in planta transformation approach. Stringent screening strategies were standardized to select putative transformants under a Cd stress
of 125 μM at both seedling and plant levels. Molecular analysis by PCR in 18 tolerant plants confirmed the transgene integration
and absence of Agrobacterium. Genomic Southern analysis further confirmed the integration of the T-DNA as a single copy. The stability of the T-DNA in
the progeny of 5 selected T1 generation plants was confirmed by tolerance assay, molecular characterization and biochemical analysis for the reduced glutathione,
phytochelatin content and lipid peroxidation. This strategy is discussed as a potential mechanism to enhance the tolerance
of rice plants to Cd stress. 相似文献
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Muralimohan N. Saini Ravi Prakash Kesiraju Karthik Pattanayak Debasis Ananda Kumar P. Kasturi K. Sreevathsa Rohini 《Journal of plant biochemistry and biotechnology.》2020,29(3):518-527
Journal of Plant Biochemistry and Biotechnology - Concurrent expression of multiple insecticidal toxins as pyramided genes in the same host plant is one of the tangible strategies to delay the... 相似文献
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K. Sankara Rao Rohini Sreevathsa Pinakee D. Sharma E. Keshamma M. Udaya Kumar 《Physiology and Molecular Biology of Plants》2008,14(4):321-328
Development of transgenics in pigeon pea remains dogged by poor plant regeneration in vitro from transformed tissues and low frequency transformation protocols. This article presents a non-tissue culture-based method of generating transgenic pigeon pea (Cajanus cajan (L.) Millisp.) plants using Agrobacterium-Ti plasmid-mediated transformation system. The protocol involves raising of whole plant transformants (T0 plants) directly from Agrobacterium-infected young seedlings. The plumular and intercotyledonary meristems of the seedling axes are targeted for transformation. The transformation conditions optimized were, pricking of the apical and intercotyledonary region of the seedling axes of two-day old germinating seedlings with a sewing needle, infection with Agrobacterium (LBA4404/pKIWI105 carrying uid A and npt II genes) in Winans’ AB medium that was added with wounded tobacco leaf extract, co-cultivation in the same medium for 1h and transfer of seedlings to soilrite for further growth and hardening and subsequent transfer of seedlings to soil in pots in the greenhouse. Out of the 22–25 primary transformants that survived infection-hardening treatments from each of the three experiments, 15 plants on the average established on the soil under greenhouse conditions, showed slow growth initially, nevertheless grew as normal plants, and flowered and set seed eventually. Of the several seeds harvested from all the T0 plants, six hundred were sown to obtain progeny (T1) plants and 350 of these were randomly analysed to determine their transgenic nature. PCR was performed for both gus (uid A) and npt II genes. Forty eight of the 350 T1 plants amplified both transgenes. Southern blot analysis substantiated the integration and transmission of these genes. The protocol ensured generation of pigeon pea transgenic plants with considerable ease in a short time and is applicable across different genotypes/cultivars of the crop and offers immense potential as a supplemental or an alternative protocol for generating transgenic plants of difficult-to-regenerate pigeon pea. Further, the protocol offers the option of doing away with a selection step in the procedure and so facilitates transformation, which is free of marker genes.Key words: Cajanus cajan, Transformation, Tissue culture-independent plant regeneration 相似文献
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Suman Sanju Aditi Thakur Sundresha Siddappa Rohini Sreevathsa Nidhi Srivastava Pradeep Shukla B. P. Singh 《Physiology and Molecular Biology of Plants》2013,19(2):165-177
The oomycete, Phytophthora infestans, is one of the most important plant pathogens worldwide. Much of the pathogenic success of P. infestans, the potato late blight agent, relies on its ability to generate large amounts of sporangia from mycelia, which release zoospores that encyst and form infection structures. Until recently, little was known about the molecular basis of oomycete pathogenicity by the avirulence molecules that are perceived by host defenses. To understand the molecular mechanisms interplay in the pathogen and host interactions, knowledge of the genome structure was most important, which is available now after genome sequencing. The mechanism of biotrophic interaction between potato and P. infestans could be determined by understanding the effector biology of the pathogen, which is until now poorly understood. The recent availability of oomycete genome will help in understanding of the signal transduction pathways followed by apoplastic and cytoplasmic effectors for translocation into host cell. Finally based on genomics, novel strategies could be developed for effective management of the crop losses due to the late blight disease. 相似文献