排序方式: 共有18条查询结果,搜索用时 93 毫秒
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Reinard Thomas; Sprunck Stefanie; Altherr Sandra; Jacobsen Hans-Jorg 《Journal of experimental botany》1994,45(11):1593-1601
A monoclonal antibody of IgM-type (TIM-11B2) was screened froma hybridoma library. The antibody recognizes a 40 kDa glycoprotein,p40, with high specificity. This protein was detected in allplant species examined so far and was found to be located bothsolubly and ionically-bound within the primary cell wall. The strongest immunobiochemical signals of p40 were found intissues undergoing elongation growth, whereas in other tissuesonly a faint signal could be detected. Those included the non-elongatingparts of different seedlings, such as the apical part of monocotprimary leaves or the leaves of dicots grown in light. Inhibitionof pea epicotyl growth by white light irradiation resulted ina strong decrease of the immunostain signal. On the other hand,induction of rapid coleoptile growth in rice seedlings inducedby submergence resulted in a strong increase of the immunobiochemicalsignal of p40. Time-course studies on the expression of p40during protoplast regeneration revealed that p40 is apparentlynot involved in cell wall formation. The hypothesis that p40is characteristic for tissues with the ability for elongationgrowth is discussed. Comparison of biochemical data and location of p40 with proteinsdescribed up to now indicate that this glycoprotein has notbeen characterized before. Key words: Cell wall protein, elongation growth, monoclonal antibody 相似文献
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One major player known to be essential for successful gamete interactions during double fertilization in Arabidopsis thaliana is the recently identified family of egg cell-secreted EC1 proteins. Both gamete fusion events are affected in EC1-deficient female gametophytes. Here, we show that the number of ovules with unfused sperm cells is considerably higher than the number of undeveloped seeds in the same ec1-RNAi knockdown lines. We found that some sperm cells are able to fuse with the female gametes even 2 to 3 days after pollination, as reflected by delayed embryo and endosperm development, and by polytubey. We propose that the egg cell secretes EC1 proteins upon sperm arrival to promote rapid sperm activation, thereby accelerating gamete fusion and preventing polytubey. 相似文献
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Stefanie Sprunck Hans-Jörg Jacobsen Thomas Reinard 《Journal of Plant Growth Regulation》1995,14(4):191-197
Indole-3-lactic acid (ILA) is a naturally occurring indole derivative, preferably detected in soil bacteria and fungi and only in low amounts in plants. T-DNA gene 5 of Agrobacterium tumefaciens was found to be involved in the synthesis of ILA in transformed plant tissues, but the physiologic relevance for ILA production in plants is unclear. The related molecular structure of ILA to the natural auxin indole-3-acetic acid (IAA) makes ILA a good candidate for an auxin analogue. We examined the possible auxin activity of ILA on elongation, proliferation, and differentiation in Pisum sativum L. Results presented in this paper indicate that there are no or only weak effects of ILA toward the activity of auxins when used in the physiologic concentration range. Furthermore, no antagonistic effects of ILA were found. Biochemical analysis using the equilibrium dialysis binding system resulted in no high affinity ILA binding to an enriched protein fraction containing auxin-binding protein (ABP44), whereas 1-naphthaleneacetic acid exhibited high affinity auxin binding.Abbreviations IAA
indoleacetic acid
- ILA
indole-3-lactic acid
- T-DNA
transferred DNA
- ABP
auxin-binding protein
- NAA
naphthaleneacetic acid
- MS
Murashige and Skoog
- MES
2-(N-morpholino)ethanesulfonic acid
- BAP
6-benzylaminopurine 相似文献
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Frank Vogler Christina Schmalzl Maria Englhart Martin Bircheneder Stefanie Sprunck 《Sexual plant reproduction》2014,27(3):153-167
Pollen tubes are among the fastest tip-growing plant cells and represent an excellent experimental system for studying the dynamics and spatiotemporal control of polarized cell growth. However, investigating pollen tube tip growth in the model plant Arabidopsis remains difficult because in vitro pollen germination and pollen tube growth rates are highly variable and largely different from those observed in pistils, most likely due to growth-promoting properties of the female reproductive tract. We found that in vitro grown Arabidopsis pollen respond to brassinosteroid (BR) in a dose-dependent manner. Pollen germination and pollen tube growth increased nine- and fivefold, respectively, when media were supplemented with 10 µM epibrassinolide (epiBL), resulting in growth kinetics more similar to growth in vivo. Expression analyses show that the promoter of one of the key enzymes in BR biosynthesis, CYP90A1/CPD, is highly active in the cells of the reproductive tract that form the pathway for pollen tubes from the stigma to the ovules. Pollen tubes grew significantly shorter through the reproductive tract of a cyp90a1 mutant compared to the wild type, or to a BR perception mutant. Our results show that epiBL promotes pollen germination and tube growth in vitro and suggest that the cells of the reproductive tract provide BR compounds to stimulate pollen tube growth. 相似文献
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Sperm competition does not occur in flowering plants as typically only a single pair of sperm cells is delivered for double fertilization. Two recent reports show that plants are capable of avoiding reproductive failure when defective sperm cells are released. 相似文献
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Martina Jurani? Kanok-orn Srilunchang Nádia Graciele Krohn Dunja Leljak-Levani? Stefanie Sprunck Thomas Dresselhaus 《The Plant cell》2012,24(12):4974-4991
Germline and early embryo development constitute ideal model systems to study the establishment of polarity, cell identity, and asymmetric cell divisions (ACDs) in plants. We describe here the function of the MATH-BTB domain protein MAB1 that is exclusively expressed in the germ lineages and the zygote of maize (Zea mays). mab1 (RNA interference [RNAi]) mutant plants display chromosome segregation defects and short spindles during meiosis that cause insufficient separation and migration of nuclei. After the meiosis-to-mitosis transition, two attached nuclei of similar identity are formed in mab1 (RNAi) mutants leading to an arrest of further germline development. Transient expression studies of MAB1 in tobacco (Nicotiana tabacum) Bright Yellow-2 cells revealed a cell cycle–dependent nuclear localization pattern but no direct colocalization with the spindle apparatus. MAB1 is able to form homodimers and interacts with the E3 ubiquitin ligase component Cullin 3a (CUL3a) in the cytoplasm, likely as a substrate-specific adapter protein. The microtubule-severing subunit p60 of katanin was identified as a candidate substrate for MAB1, suggesting that MAB1 resembles the animal key ACD regulator Maternal Effect Lethal 26 (MEL-26). In summary, our findings provide further evidence for the importance of posttranslational regulation for asymmetric divisions and germline progression in plants and identified an unstable key protein that seems to be involved in regulating the stability of a spindle apparatus regulator(s). 相似文献