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Priyanka Fernandes Manon Loubens Rmi Le Borgne Carine Marinach Batrice Ardin Sylvie Briquet Laetitia Vincensini Soumia Hamada Bndicte Hoareau-Coudert Jean-Marc Verbavatz Allon Weiner Olivier Silvie 《PLoS pathogens》2022,18(6)
Plasmodium sporozoites that are transmitted by blood-feeding female Anopheles mosquitoes invade hepatocytes for an initial round of intracellular replication, leading to the release of merozoites that invade and multiply within red blood cells. Sporozoites and merozoites share a number of proteins that are expressed by both stages, including the Apical Membrane Antigen 1 (AMA1) and the Rhoptry Neck Proteins (RONs). Although AMA1 and RONs are essential for merozoite invasion of erythrocytes during asexual blood stage replication of the parasite, their function in sporozoites was still unclear. Here we show that AMA1 interacts with RONs in mature sporozoites. By using DiCre-mediated conditional gene deletion in P. berghei, we demonstrate that loss of AMA1, RON2 or RON4 in sporozoites impairs colonization of the mosquito salivary glands and invasion of mammalian hepatocytes, without affecting transcellular parasite migration. Three-dimensional electron microscopy data showed that sporozoites enter salivary gland cells through a ring-like structure and by forming a transient vacuole. The absence of a functional AMA1-RON complex led to an altered morphology of the entry junction, associated with epithelial cell damage. Our data establish that AMA1 and RONs facilitate host cell invasion across Plasmodium invasive stages, and suggest that sporozoites use the AMA1-RON complex to efficiently and safely enter the mosquito salivary glands to ensure successful parasite transmission. These results open up the possibility of targeting the AMA1-RON complex for transmission-blocking antimalarial strategies. 相似文献
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Touria Hssaida Soumia Chahidi Mohamed Benzaggagh James B. Riding Fatima Oumalch 《Annales de Paléontologie》2014
A biostratigraphic study of dinoflagellate cysts of the Upper Jurassic series from the eastern External Rif Chain, has been achieved for the first time on four outcrop sections : the DM section (Douar Marticha), the TB section (the Tarhchenna “Sof”), the KSD section (the Kef Mallou “Sof”) and the Y section (Douar Lamriene) among which three sections (TB, KSD and Y) are well-dated by ammonites and calpionellids fauna. The DM and TB sections are complementary in terms of stratigraphy and correspond respectively to the upper part of the “Ferrysch” Formation, a thick shaly–silty and sandy sequence assigned to the Callovian-Oxfordian stages and to the El Gouzat limestones Formation (Kimmeridgian - Lower Tithonian age) and to the Tarhchenna marly limestones Formation (Upper Tithonian age) from the EL Gouzat area (Mesorif). The KSD and Y sections are also complementary and correspond to the Kimmeridgian-Lower Tithonian Msila limestones Formation and to the overlying marly limestones Massif Formation of Upper Tithonian age, outcropping in the Msila region (Internal Prerif). These four sections have shown many dinocysts rich levels, which had allowed characterizing the Middle? and Upper Oxfordian substages in the DM section. In the other sections, the dinoflagellate cyst assemblages are nearly similar to those of the Kimmeridgian–Tithonian of many sections from the Boreal, Sub-boreal and North tethyan realms, particularily from England, Russia and South-East France basin. The results of the present work confirm once again, the important value of dinoflagellate cysts, for age dating of ammonites-lacking Mesozoic sedimentary sequences and for biostratigraphic correlations. 相似文献
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José C. Brito Raquel Godinho Fernando Martínez‐Freiría Juan M. Pleguezuelos Hugo Rebelo Xavier Santos Cândida G. Vale Guillermo Velo‐Antón Zbyszek Boratyński Sílvia B. Carvalho Sónia Ferreira Duarte V. Gonçalves Teresa L. Silva Pedro Tarroso João C. Campos João V. Leite Joana Nogueira Francisco Álvares Neftalí Sillero Andack S. Sow Soumia Fahd Pierre‐André Crochet Salvador Carranza 《Biological reviews of the Cambridge Philosophical Society》2014,89(1):215-231
Deserts and arid regions are generally perceived as bare and rather homogeneous areas of low diversity. The Sahara is the largest warm desert in the world and together with the arid Sahel displays high topographical and climatic heterogeneity, and has experienced recent and strong climatic oscillations that have greatly shifted biodiversity distribution and community composition. The large size, remoteness and long‐term political instability of the Sahara‐Sahel, have limited knowledge on its biodiversity. However, over the last decade, there have been an increasing number of published scientific studies based on modern geomatic and molecular tools, and broad sampling of taxa of these regions. This review tracks trends in knowledge about biodiversity patterns, processes and threats across the Sahara‐Sahel, and anticipates needs for biodiversity research and conservation. Recent studies are changing completely the perception of regional biodiversity patterns. Instead of relatively low species diversity with distribution covering most of the region, studies now suggest a high rate of endemism and larger number of species, with much narrower and fragmented ranges, frequently limited to micro‐hotspots of biodiversity. Molecular‐based studies are also unravelling cryptic diversity associated with mountains, which together with recent distribution atlases, allows identifying integrative biogeographic patterns in biodiversity distribution. Mapping of multivariate environmental variation (at 1 km × 1 km resolution) of the region illustrates main biogeographical features of the Sahara‐Sahel and supports recently hypothesised dispersal corridors and refugia. Micro‐scale water‐features present mostly in mountains have been associated with local biodiversity hotspots. However, the distribution of available data on vertebrates highlights current knowledge gaps that still apply to a large proportion of the Sahara‐Sahel. Current research is providing insights into key evolutionary and ecological processes, including causes and timing of radiation and divergence for multiple taxa, and associating the onset of the Sahara with diversification processes for low‐mobility vertebrates. Examples of phylogeographic patterns are showing the importance of allopatric speciation in the Sahara‐Sahel, and this review presents a synthetic overview of the most commonly hypothesised diversification mechanisms. Studies are also stressing that biodiversity is threatened by increasing human activities in the region, including overhunting and natural resources prospection, and in the future by predicted global warming. A representation of areas of conflict, landmines, and natural resources extraction illustrates how human activities and regional insecurity are hampering biodiversity research and conservation. Although there are still numerous knowledge gaps for the optimised conservation of biodiversity in the region, a set of research priorities is provided to identify the framework data needed to support regional conservation planning. 相似文献
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In recent decades, fires in Mediterranean Europe have become larger and more frequent. This trend has been driven by socioeconomic changes that have generated rural depopulation and changes in traditional land use. Within the Mediterranean Basin, the most contrasting socioeconomic conditions are found by comparing southern European with North African countries, and thus our hypothesis is that this difference generates contrasting fire regimes between the two regions. Specifically, we predict that current fire regimes in Mediterranean Africa resemble past fire regimes in the Mediterranean Europe when rural activities dominated the landscape. To test our hypothesis, we compared fire statistics from the western Rif (northern Morocco, 1988–2015) and from Valencia (eastern Spain, 1880–2014). The results suggest that the Rif has a typical Mediterranean fire regime with fires occurring in the hot, dry summer season. However, fires are very small and the annual proportion of burnt area is very low, compared to the current regime in Valencia (post-1970s). The current Rif fire size class distribution matches the fire regime in Valencia prior to the 1970s before the collapse of the rural population and when fires were fuel-limited. The shift in the recent decades in fire regime observed in different countries of the Mediterranean Europe (from small, fuel-limited fires to drought-driven fires) can be identified when moving from the southern to the northern rim of the basin. That is, most spatial and temporal variability in fire regimes of the Mediterranean Basin is driven by shifts in the amounts of fuel and continuity imposed by changes in socioeconomic drivers. 相似文献
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Heiko Stuckas Guillermo Velo-Antón Soumia Fahd Mohsen Kalboussi Rachid Rouag Marco Arculeo Federico Marrone Francesco Sacco Melita Vamberger Uwe Fritz 《Organisms Diversity & Evolution》2014,14(3):295-306
The European pond turtle (Emys orbicularis) is a Nearctic element in the African fauna and thought to have invaded North Africa from the Iberian Peninsula. All North African populations are currently identified with the subspecies E. o. occidentalis. However, a nearly range-wide sampling in North Africa used for analyses of mitochondrial and microsatellite DNA provides evidence that only Moroccan populations belong to this taxon, while eastern Algerian and Tunisian pond turtles represent an undescribed distinct subspecies. These two taxa are most closely related to E. o. galloitalica with a native distribution along the Mediterranean coast of northern Spain through southern France to western and southern Italy. This group is sister to a clade comprising several mitochondrial lineages and subspecies of E. orbicularis from Central and Eastern Europe plus Asia, and the successive sisters are E. o. hellenica and E. trinacris. Our results suggest that E. orbicularis has been present in North Africa longer than on the Iberian Peninsula and that after an initial invasion of North Africa by pond turtles from an unknown European source region, there was a phase of diversification in North Africa, followed by a later re-invasion of Europe by one of the African lineages. The differentiation of pond turtles in North Africa parallels a general phylogeographic paradigm in amphibians and reptiles, with deeply divergent lineages in the western and eastern Maghreb. Acknowledging their genetic similarity, we propose to synonymize the previously recognized Iberian subspecies E. o. fritzjuergenobsti with E. o. occidentalis sensu stricto. The seriously imperiled Moroccan populations of E. o. occidentalis represent two Management Units different in mitochondrial haplotypes and microsatellite markers. The conservation status of eastern Algerian pond turtles is unclear, while Tunisian populations are endangered. Considering that Algerian and Tunisian pond turtles represent an endemic taxon, their situation throughout the historical range should be surveyed to establish a basis for conservation measures. 相似文献
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Ayoub Haouas Cherkaoui El Modafar Allal Douira Saâd Ibnsouda-Koraichi Abdelkarim Filali-Maltouf Abdelmajid Moukhli Soumia Amir 《Saudi Journal of Biological Sciences》2021,28(1):371-379
The isolation and identification of beneficial bacteria from the active phase of composting is considered to be a key bio-quality parameter for the assessment of the process. The aim of this work was the selection and identification of beneficial bacteria from a co-composting experiment of vegetable waste (VW), olive oil mill waste (O2MW), and phosphate sludge (PS). Phosphate-solubilizing strains were isolated from the thermophilic phase using Pikovskaya (PVK) solid medium supplemented with tricalcium phosphate Ca3(PO4) (TCP) as the sole source of phosphorus (P). Therefore, the selected isolate Alcaligenes aquatilis GTE53 was tested to tolerate abiotic stresses (different levels of temperature, variable pH, high salinity and water stress). The isolate was also assessed for indole acetic acid (IAA) and siderophores synthesis, nitrogen fixation, phenol degradation and pathogens inactivation. The quality of the co-composting process was also investigated by monitoring the physico-chemical parameters. The obtained results showed that A. aquatilis GTE53 displayed a higher solubilization index of 2.4 and was efficiently dissolved, up to 162.8 and 247.4 mg·mL−1 of inorganic phosphate from PS and phosphate rock (PR), respectively. A. aquatilis GTE53 exhibited siderophores and IAA release, along with atmospheric nitrogen fixation. In addition to that, A. aquatilis GTE53 showed a high resistance to heat and tolerance to acidic and alkaline pH, high salinity and water stress. Moreover, A. aquatilis GTE53 could degrade 99.2% of phenol from a high-concentrated medium (1100 mg·L−1 of phenol) and can inactivate the most abundant pathogens in industrial wastes: Escherichia coli, Streptococcus sp., Salmonella sp., and Fusarium oxysporum albedinis. Analysis of temperature, pH, electrical conductivity, carbon/nitrogen (C/N) ratio, indicated successful co-composting. An efficient transformation of P to the available form and a great abatement of polyphenols, were also recorded during the process. The findings of this study will help to advance the understanding of A. aquatilis GTE53 functions and will facilitate its application to promote beneficial microbial organisms during composting, thus obtaining a high-quality product. 相似文献
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The fulvic acid fraction as it changes in the mature phase of vegetable oil-mill sludge and domestic waste composting 总被引:4,自引:1,他引:3
Abouelwafa R Amir S Souabi S Winterton P Ndira V Revel JC Hafidi M 《Bioresource technology》2008,99(14):6112-6118
Sludge resulting from the treatment of effluent from a vegetable oil mill, was composted mixed with domestic waste in a pile for five months. Different proportions of sludge and dry waste were mixed: M1 (1v/2v) and M2 (1v/1v). Monitoring different physical-chemical parameters showed the effect of the substrate on the microbiological activity and on the formation of fulvic acids, affecting the maturity of the final compost. Elemental analysis revealed that the fulvic acids of mixes M1 and M2 presented very low concentrations of carbon, hydrogen, and oxygen and a high level of nitrogen. The FTIR spectroscopy results showed a decrease during composting of the intensity of absorbance of the easily assimilable compounds that are predominant in the initial mixtures i.e. the carbohydrates (1170-1080 cm(-1)) in M1 and long aliphatic chains (2920 cm(-1)) in M2. For mix M1 there was enrichment in compounds bearing oxygen-containing moieties. In M2 it was the nitrogen-containing compounds (in the form of stable amides) which predominated at the end of composting. The first component of PCA analysis, PC1, accounted for 83% of the difference between two distinct groups of parameters governing degradation and restructuration of the fulvic acids during composting. PC2 (17%) explained the variance due to the level of free or less polycondensed compounds in the two mixtures. Oxidised polyphenolic and polysaccharide structures were the least free, or most polycondensed, in the fulvic structures of M1. In M2 fulvic acids however, it was the polyphenols and peptide structures that were involved in the bonding, most likely of the polyphenol-peptide type. 相似文献
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Pleguezuelos JM Santos X Brito JC Parellada X Llorente GA Fahd S 《Zoology (Jena, Germany)》2007,110(1):9-19
Eurosiberian vipers have been considered model organisms, and studies on their reproductive ecology have afforded much of the current knowledge concerning viviparity in snakes. However, such studies are biased towards northern species and there is little information on Mediterranean species and/or populations. The reproductive ecology of Vipera latastei in the Iberian Peninsula was studied by analysing a large sample of specimens from collections, to better understand the conservation status of this Mediterranean viper. Males and females matured at small and similar body sizes (240 and 265 mm snout-vent length, respectively) and reproductive cycles in both sexes were seasonal. Spermatogenesis peaked in August, vitellogenesis developed in spring and the timing of the mating period was puzzling, with populations mating in autumn, spring, or in both seasons. The most striking finding was that adult females reproduced triennially on average. Lataste's viper is currently in continuous decline in the IP, and most of its populations are isolated in Mediterranean mountains. We hypothesize that prey scarcity and the brevity of the activity period in mountain habitats diminishes the ability of vipers to recover over the short term the energy expended in reproduction. The species needs 2 years for the acquisition and storage of energy ("capital breeder"), and a third year for the expenditure of this energy (in vitellogenesis and embryogenesis), a year during which females feed consistently ("income breeder"). Thus, this viper combines both strategies to supply the reproductive energy cost. Current decline in population and distribution, together with a poor capacity to renew populations, renders Lataste's viper vulnerable to environmental stochasticity. 相似文献
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