Flax in Neolithic and Bronze Age Greece: archaeobotanical evidence

This paper presents an overview of archaeobotanical finds of Linum usitatissimum from Neolithic and Bronze Age Greece, bringing together published records of this plant as well as some recently retrieved, unpublished finds. In addition, charred flax seed concentrations from five prehistoric sites from the region of Macedonia in northern Greece, are examined in detail. The Neolithic sites are Makriyalos, Mandalo, Arkadikos, Dikili Tash, dated to the Late and Final Neolithic and Archondiko, dated to the end of the Early Bronze Age. Archaeobotanical composition and contextual information are used in order to explore the cultivation and potential uses of flax at each site. By the 5th millennium b.c. a flax weed flora had probably developed in the region. The use of flax seed for oil extraction and flax stems for flax fibre preparation can only be speculated upon and these uses are discussed within the context of other archaeological finds related to plant oil production and weaving. It is also possible that flax may have been used for medicinal purposes. Despite a striking paucity in archaeobotanical remains from southern Greece, textual evidence available from the Mycenean palace archives in Linear B clearly documents the cultivation of flax and flax fibre production.     

Bcl-2 but not clusterin/apolipoprotein J protected human diploid fibroblasts and immortalized keratinocytes from ceramide-induced apoptosis: role of p53 in the ceramide response

The role of clusterin/apolipoprotein J (Clu/ApoJ) and Bcl-2 on C(2)-ceramide-induced apoptosis of embryonic human diploid fibroblasts, MRC-5 and immortalized adult skin keratinocytes, HaCaT was investigated. C(2)-ceramide-induced apoptosis of HaCaT in a time- and dose-dependent manner, while in MRC-5 only at higher concentrations. There was a dose-dependent accumulation of Clu/ApoJ and downregulation of Bcl-2 which correlated with C(2)-ceramide-induced apoptosis of MRC-5. While overexpression of Bcl-2 suppressed C(2)-ceramide-mediated apoptosis in both cell types, Clu/ApoJ failed to do so, accessed by morphological changes, DNA fragmentation and PARP cleavage. There was no change in the expression of endogenous p53 or p21(Waf1/Cip1) upon C(2)-ceramide treatment of MRC-5. However, mutant p53(143ala) increased the sensitivity of MRC-5 to C(2)-ceramide-induced apoptosis by markedly downregulating Bcl-2, pointing to a role for p53. These results suggested that whereas downregulation of Bcl-2 may be a crucial factor involved in C(2)-ceramide-induced apoptosis, accumulation of Clu/ApoJ may be a signal of stress response. Moreover, the ceramide-activated apoptotic pathway may be regulated by p53.     

Grain versus chaff: identifying a contrast between grain-rich and chaff-rich sites in the Neolithic of northern Greece

Recent archaeobotanical investigations of Late Neolithic sites in northern Greece have revealed a pattern as regards the crop parts represented at each site. Some sites appear to be dominated by chaff, mainly glume wheat chaff, while other sites are mainly characterised by cereal grain and pulses. This pattern could be the outcome of various factors: a. pre-depositional differential treatment of crops selecting for one category of plant remains to arrive at the site, b. depositional, therefore related to the type of activity represented at the excavated contexts and the use of space in relation to plants, and c. post-depositional factors such as erosion, or the type of features sampled. These alternative factors are considered in light of the preliminary results of the archaeobotanical investigation of seven Late Neolithic (5500–4000 cal. B.C.) sites from northern Greece. It is suggested that this pattern is the outcome of a differential use of space in relation to plants (storage, processing/food preparation, refuse disposal), probably related to aspects of the socioeconomic organisation of the settlements.     

An investigation of processing and consumption of pulses among prehistoric societies: archaeobotanical, experimental and ethnographic evidence from Greece

Pulses have constituted an important food source for prehistoric communities in the Old World, yet little is known as regards their processing for consumption through the archaeobotanical record. This paper provides an overview of archaeobotanical evidence for the use of pulses in prehistoric Greece based on two case studies from the north, and explores (a) their preparation for consumption, in particular their detoxification and (b) the consumption of pulses as a component of ordinary daily meals in prehistoric times, as well as those for special occasions, within a context of feasting and ritual. The paper examines charred remains of Vicia ervilia (bitter vetch) and Lathyrus sativus (grass pea) from early Bronze Age Agios Athanasios and late Neolithic Kremasti Koiladas, respectively, as the former provides a basis for a pilot exploration of pulse detoxification and the latter, due to its origin, offers a rare opportunity to discuss the context of consumption. In the pilot exploration of pulse seed preparation for consumption, the inner cotyledon morphology of modern V. ervilia seeds which were experimentally processed with water and pounding was examined macroscopically and through SEM micrographs. Preliminary observations suggest that intentional splitting of pulse seeds as part of processing for consumption as food may be recognisable in the archaeobotanical record. Processing with water may also be detected. The particular context of the Kremasti finds suggests that pulses, in this particular case L. sativus, may have constituted special foods for particular occasions, loaded with symbolic meaning.     

INCREASED CONCENTRATION OF SOIL CADMIUM AFFECTS ON PLANT GROWTH,DRY MATTER ACCUMULATION,Cd, AND Zn UPTAKE OF DIFFERENT TOBACCO CULTIVARS (NICOTIANA TABACUM L.)

Cadmium (Cd) is a toxic trace metal pollutant for humans, animals, and plants. Tobacco is a wellknown efficient accumulator of Cd and the genotypic differences in Cd uptake and the response to Cd was not determined. The objectives of this study were to investigate: 1) the effects of Cd on the growth and development of different tobacco cultivars; 2) the differences among tobacco cultivars in Cd concentration, uptake, and use for the phytoremediation of polluted soils with Cd; and (3) the interactions between Cd and Zn with respect to concentration and uptake. The Cd level affected the number of leaves and dry matter accumulation, and there were differences among the different cultivars that were used. Furthermore, some cultivars showed a higher reduction in growth than others, indicating that they are more sensitive to Cd level in the soil. Moreover, differences existed among the cultivars for the Cd concentration and uptake. There also were negative correlations between Cd and Zn concentrations; as Cd accumulation increased, Zn accumulation decreased, which showed that the two heavy metals were antagonistic. These results suggest that tobacco cultivars differed greatly in their growth and developmental responses to Cd and in the concentration and uptake of Cd and Zn. In addition, it is possible to use certain tobacco cultivars to lower the Cd concentration in the soil.     

Brewing beer in wine country? First archaeobotanical indications for beer making in Early and Middle Bronze Age Greece

This paper revisits and old question “Beer or wine?” as regards the potential alcoholic drinks consumed by prehistoric societies in southeastern Europe. Archaeobotanical remains of sprouted cereal grains as well as cereal fragments from the Bronze Age sites of Archondiko and Argissa on mainland Greece, presented here for the first time, provide strong indications for the making of something similar to beer in late 3rd millennium bc Greece, opening up a series of new questions about the recipes followed in this process and their origins. Beyond the recipes themselves, the paper highlights a range of available options as regards alcoholic drinks in Bronze Age Greece, beer and wine, offering thus a more detailed approach to preferences and possible identities reflected in the choice of alcoholic drink among prehistoric societies inhabiting the southernmost tip of the Balkan Peninsula, the Aegean and mainland Greece.     

Major histocompatibility complex class I restricted T-cell autoreactivity in human peripheral blood mononuclear cells

During selection in the thymus or any subsequent response, T-cells recognize peptides bound to major histocompatibility complex (MHC) molecules. Peptides produced by lysosomes or by proteasome/immunoproteasome stimulate CD4+ or CD8+ T-cell, respectively. Inflammation alters components of both antigen-processing pathways resulting in the production of different peptides. The role of such changes in self/non-self discrimination was examined in autologous mixed peripheral blood mononuclear cell cultures. Stimulator cells were incubated in the presence or absence of INF-gamma, with or without lysosome inhibitors (ammonium chloride/chloroquine), cathepsin inhibitor (E-64), or proteasome/immunoproteasome inhibitor (epoxomicin). Responder cells were added and zeta-chain phosphorylated forms were used as read out. INF-gamma did not affect zeta-chain phosphorylated forms, which means that the expected INF-gamma induced alterations in antigen processing machinery do not influence self/non-self discrimination. Surprisingly, the completely phosphorylated 23-kDa zeta-chain was always present except in the case of epoxomicin, indicating the presence of MHC class I restricted autoreactive CD8+ T-cells but not of MHC class II restricted autoreactive CD4+ T-cells, possibly due to more efficient negative selection in the thymus of the latter. Autoimmunity is prevented due to absence of help by CD4+ T-cells. This conclusion was confirmed by the lack of differences in IL-2 levels in cell culture supernatants, as well as, by the absence of differences in cell proliferation under the various conditions described above.     

An experimental approach for tracing olive processing residues in the archaeobotanical record, with preliminary examples from Tell Tweini, Syria

To help the interpretation of possible olive processing residues at archaeological sites, this study examines the changes which occur in pre- and post-charring breakage surfaces of olive stones at tissue level. Fresh olives were experimentally broken before and after charring and heated to different temperatures (230, 330, 430°C) in oxidising and non-oxidising conditions. The structures obtained by the experiment were studied with scanning electron microscopy (SEM) and the characteristics for the different temperatures, oxidation regimes and pre- or post-charring fractures were recorded. Furthermore, the experimental specimens were compared with recent and possibly old fractures of several archaeological olive stones from Tell Tweini, Syria. Criteria to infer their formation are discussed. These criteria could be developed further through more experimental replication and additional charring variables, and verified extensively on archaeobotanical assemblages in future studies. The described structures and alterations in the olive stones can be observed using reflected light microscopy, which would allow the proposed approach to be rather easy to apply in practice.