全文获取类型
收费全文 | 287篇 |
免费 | 15篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 12篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 9篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 23篇 |
2014年 | 20篇 |
2013年 | 22篇 |
2012年 | 21篇 |
2011年 | 22篇 |
2010年 | 17篇 |
2009年 | 13篇 |
2008年 | 14篇 |
2007年 | 10篇 |
2006年 | 10篇 |
2005年 | 9篇 |
2004年 | 7篇 |
2003年 | 9篇 |
2002年 | 7篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有302条查询结果,搜索用时 15 毫秒
1.
2.
B. Nicolaus A. Trincone L. Lama I. Romano F. Marsiglia A. Gambacorta 《Biotechnology letters》1991,13(9):667-670
Summary An economic method to grow the thermoacidophilic archaebacteriumSulfolobus solfataricus is reported. 相似文献
3.
4.
“Bacillus thermoantarcticus” sp. nov., from Mount Melbourne,Antarctica: a novel thermophilic species
B. Nicolaus Licia Lama Enrico Esposito Maria Cristina Manca Guido di Prisco Agata Gambacorta 《Polar Biology》1996,16(2):101-104
A novel thermophilic Gram-positive bacillus, “Bacillus thermoantarcticus”, isolated from geothermal soil near the crater of Mount Melbourne, is described. The organism grows at an optimal temperature
of 63°C at pH 6.0, is oxidase-positive, catalase-negative and produces an exopolysaccharide, an exocellular xylanase, an intracellular
alcohol dehydrogenase and exo- and endocellular α-glucosidase(s). The sequence of 16S rDNA is very similar to that of “Bacillus thermoglucosidasius”; however, the guanine-plus-cytosine (G+C) content is 8 mol% higher. The type strain is “Bacillus thermoantarcticus” (DSM 9572).
Received : 3 February 1995/Accepted : 12 May 1995 相似文献
5.
Summary
Mortierella alpina S-17 cultivated in shaker flasks in the semisynthetic liquid medium with 3% glucose at 28 °C for 14 days accumulated 2.1 g/l of intracellular lipid rich in arachidonic acid (ARA). The content of ARA was 50 % of the total fatty acids. The simple method for high pure methyl arachidonate preparation was developed. 相似文献
6.
Trincone A Lama L Lanzotti V Nicolaus B De Rosa M Rossi M Gambacorta A 《Biotechnology and bioengineering》1990,35(6):559-564
The method of resting cells has been of interest in the development of biocatalysts applied to organic reactions.This article deals with the use of resting cells of a thermophilic archaebacterium Sulfolobus solfataricus, in the asymmetric reduction of acyclic, cyclic, and aromatic ketones. The system allows the continuous regeneration of endogenous coenzyme with the coupled substrate approach. The results indicate that the direction of hydride attack was equatorial on the re face of the carbonyl group of substrates producing (S)-alcohols with a good optical yield. A convenient system for the reuse of resting cells has been set out to synthesize (S)-alcohols on a preparative scale. 相似文献
7.
8.
Léo Correia da Rocha Filho Natália de Campos Muradas Cerântola Carlos Alberto Garófalo Vera Lúcia Imperatriz-Fonseca Marco Antonio Del Lama 《Genetica》2013,141(1-3):65-74
Euglossini bees are among the main pollinators of plant species in tropical and subtropical forests in Central and South America. These bees are known as long-distance pollinators due to their exceptional flight performance. Here we assessed through microsatellite loci the gene variation and genetic differentiation between populations of four abundant Euglossini species populations sampled in two areas, Picinguaba (mainland) and Anchieta Island, Ubatuba, São Paulo State, southeastern Brazil. There was no significant genetic differentiation between the island and mainland samples of Euglossa cordata (Fst = 0.008, P = 0.60), Eulaema cingulata (Fst = 0.029, P = 0.29) and Eulaema nigrita (Fst = 0.062, P = 0.38), but a significant gene differentiation between mainland and island samples of Euglossa stellfeldi (Fst = 0.028, P = 0.016) was detected. As expected, our results showed that the water body that separates the island from the mainland does not constitute a geographic barrier for these Euglossini bees. The absence of populational structuring of three out the four species studied corroborates previous reports on those bees, characterized by large populations, with high gene diversity and gene flow and very low levels of diploid males. But the Eg. stellfeldi results clearly point that dispersal ability is not similar to all euglossine bees, what requires the development of different conservationist strategies to the Euglossini species. 相似文献
9.
Rati Lama Lin Zhang Janine M. Naim Jennifer Williams Aimin Zhou Bin Su 《Bioorganic & medicinal chemistry》2013,21(4):922-931
It has been demonstrated that two-dimensional (2D) monolayer cancer cell proliferation assay for anti-cancer drug screening is a very artificial model and cannot represent the characteristics of three-dimensional (3D) solid tumors. The multi-cellular in vitro 3D tumor spheroid model is of intermediate complexity, and can provide a bridge to the gap between the complex in vivo tumors and simple in vitro monolayer cell cultures. In this study, a simple and cost-effective cancer 3D spheroid assay suitable for small molecule anti-cancer compound screening was developed, standardized and validated on H292 non-small lung cancer cell line. A pilot screening with this assay was performed utilizing a compound library consisting of 41 anti-cancer agents. The traditional 2D monolayer cell proliferation assay was also performed with the same cell line and compounds. A correlational study based on the IC50 values from the 2D and 3D assays was conducted. There is low correlation with the two sets of biological data, suggesting the two screening methods provide different information regarding the potency of the tested drug candidates. 相似文献
10.
Vishal Nanavaty Rati Lama Ranjodh Sandhu Bo Zhong Daniel Kulman Viharika Bobba Anran Zhao Bibo Li Bin Su 《PloS one》2016,11(1)