全文获取类型
收费全文 | 4519篇 |
免费 | 326篇 |
国内免费 | 1篇 |
出版年
2023年 | 26篇 |
2022年 | 64篇 |
2021年 | 110篇 |
2020年 | 77篇 |
2019年 | 80篇 |
2018年 | 138篇 |
2017年 | 109篇 |
2016年 | 186篇 |
2015年 | 232篇 |
2014年 | 275篇 |
2013年 | 353篇 |
2012年 | 387篇 |
2011年 | 371篇 |
2010年 | 239篇 |
2009年 | 204篇 |
2008年 | 276篇 |
2007年 | 272篇 |
2006年 | 249篇 |
2005年 | 239篇 |
2004年 | 220篇 |
2003年 | 201篇 |
2002年 | 181篇 |
2001年 | 38篇 |
2000年 | 32篇 |
1999年 | 32篇 |
1998年 | 38篇 |
1997年 | 27篇 |
1996年 | 16篇 |
1995年 | 11篇 |
1994年 | 14篇 |
1993年 | 12篇 |
1992年 | 11篇 |
1991年 | 16篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 11篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 10篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1976年 | 3篇 |
1975年 | 6篇 |
1973年 | 3篇 |
1969年 | 2篇 |
排序方式: 共有4846条查询结果,搜索用时 15 毫秒
1.
Shull A. Franklin und Ladoff Sonia 《Molecular & general genetics : MGG》1918,19(1-2):110-115
2.
Legionella pneumophila in Cooling Towers: Fluctuations in Counts, Determination of Genetic Variability by Pulsed-Field Gel Electrophoresis (PFGE), and Persistence of PFGE Patterns
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sonia Ragull Marian Garcia-Nuez Maria Luisa Pedro-Botet Nieves Sopena Maria Esteve Rafael Montenegro Miquel Sabri 《Applied microbiology》2007,73(16):5382-5384
The concentrations of Legionella pneumophila in cooling towers may vary considerably over short periods of time, producing significant fluctuations throughout the year. Despite genetic variability, in small geographical areas the same indistinguishable pulsed-field gel electrophoresis patterns may be shared among different cooling towers and persist over time. 相似文献
3.
Sonia Coni Silvia Maria Serrao Zuleyha Nihan Yurtsever Laura Di Magno Rosa Bordone Camilla Bertani Valerio Licursi Zaira Ianniello Paola Infante Marta Moretti Marialaura Petroni Francesca Guerrieri Alessandro Fatica Alberto Macone Enrico De Smaele Lucia Di Marcotullio Giuseppe Giannini Marella Maroder Enzo Agostinelli Gianluca Canettieri 《Cell death & disease》2020,11(12)
4.
Biosorption is becoming an important component in the integrated approach to the treatment of aqueous effluents. The economics of biomass technical applications are improved by using waste biomass instead of purposely-produced biomass. Biomass derived from an alginate extraction industry - Sargassum sp. - was examined for its ability to function as a biosorbent for metals such as cadmium, zinc and copper. For use in column applications, biomass should be immobilized. To the algae reinforcement, the biomass was embedded in polyethleneimine (PEI), followed by glutaraldehyde crosslinking. Equilibrium Zn and Cu isotherms were analysed using the immobilization ratio that showed the best Cd performance. Either Freundlich or Langmuir models can describe the passive biosorption equilibrium of cadmium, zinc and cooper. The preference for this series of metals by the biomass was found to be Cd > Zn > Cu, with maximum uptake values of 157.8, 118.5 and 77.4 mg/g dry weight biomass for Cd, Zn and Cu. respectively. The metal binding capacity by non-living biomass is an important quality for industrial use. 相似文献
5.
6.
Jean L. Santos Paulo R. Yamasaki Chung Man Chin Célio H. Takashi Fernando R. Pavan Clarice Q.F. Leite 《Bioorganic & medicinal chemistry》2009,17(11):3795-3799
New phthalimide derivatives were easily prepared through condensation of phthalic anhydride and selected amines with variable yields (70–90%). All compounds (3a–l) were evaluated against Mycobacterium tuberculosis H37Rv using Alamar Blue susceptibility. The compounds 3c, 3i, and 3l have the minimum inhibitory concentrations (MICs) of 3.9, 7.8, and 5.0 μg/mL, respectively, and could be considered new lead compounds in the treatment of tuberculosis and multi-drug resistant tuberculosis. 相似文献
7.
Elisabeth S. Oliveira Paulo E. P. Leite Maria G. Spillantini † Antonio C. M. Camargo Stephen P. Hunt† 《Journal of neurochemistry》1990,55(4):1114-1121
The subcellular and regional distribution of endo-oligopeptidase (EC 3.4.22.19), an enzyme capable of generating enkephalin by single cleavage from enkephalin-containing peptides, was determined by an enzymatic assay using metorphamide and by immunochemical techniques in the CNS of the rat. The rat CNS contains a membrane-associated form of endo-oligopeptidase, an enzyme predominantly associated with the soluble fraction of brain homogenates. Subcellular fractionation showed that approximately 17% of the total activity of the enzyme is associated with membrane fractions including synaptosomes. Synaptosomal membranes were prepared from neocortex, striatum, hypothalamus, medulla, spinal cord, and cerebellum. The amount of EC 3.4.22.19 activity solubilized by 3-[( 3-cholamidopropyl]dimethylammonio)-1-propanesulfonate from synaptosomal membranes was similar in neocortex, striatum, and hypothalamus, being three- to 10-fold greater than in spinal cord, cerebellum, and medulla. A polyclonal antibody exhibiting high affinity for endo-oligopeptidase was raised in rabbits against the purified rat brain enzyme and used to localize endo-oligopeptidase by Western blotting and by immunoperoxidase techniques. A strong band corresponding to the Mr of EC 3.4.22.19 was found in solubilized proteins obtained from synaptosomal membranes prepared from hypothalamus, neocortex, and striatum when subjected to Western blotting. The immunohistochemical localization of endo-oligopeptidase indicated that the immunoreactivity was confined to gray matter in regions known to be rich in peptide-containing neurons such as the striatum. In the cerebellum, a region poor in peptides, no staining could be detected. The nonuniform distribution of endo-oligopeptidase in rat brain suggests a role in neurotransmitter processing in the CNS. 相似文献
8.
The morphological changes in the gill chloride cells of the armored catfish, Hypostomus tietensis , were investigated after 15 days' exposure to either distilled or hard water. The thickness of the water–blood barrier in the lamellae increased significantly in fish kept in distilled water due to the high proliferation of chloride cells. The apical surface of about 68% of chloride cells was sharply reduced by the development of an apical crypt with a sponge-like surface, although no change in the chloride cell fractional area was found. In contrast, H. tietensis kept in Na+ , Cl− and Ca2+ rich water displayed no significant changes in the number of chloride cells or in their apical surface morphology compared with the control fish. Chloride cell response to ion challenge in H. tietensis suggested the involvement of different strategies to maintain homeostasis in ion-poor water, which may be related to the life history of species. 相似文献
9.
10.
Viesturs UE Strikauska SV Leite MP Berzins AJ Tengerdy RP 《Biotechnology and bioengineering》1987,30(2):282-288
A novel two-stage bioreactor has been designed for a combined submerged (SF) and solid substrate fermentation (SSF) of wheat straw. The straw was pretreated with steam, and cellulases from the culture fluid of Trichoderma reesei were adsorbed on it for increased bioconvertibility. SSF was conducted in the top part of the bioreactor by inoculating the straw with a 36-h mycelial culture of T. reesei, or Coriolus versicolor. In the bottom part of the fermenter, Endomycopsis fibuliger was grown in SF. The SF liquor was recirculated through the SSF stage at 24 h intervals to remove glucose and other metabolites that may inhibit growth, and to maintain optimum moisture level and temperature. The removed glucose and other metabolites provided nutrients for the yeast in the SF stage. The combined fermentation resulted in overall higher biomass yield, increased bioconversion, increased cellulase production, and increased digestibility compared with single SSF or SF. 相似文献