全文获取类型
收费全文 | 423篇 |
免费 | 125篇 |
国内免费 | 3篇 |
专业分类
551篇 |
出版年
2019年 | 3篇 |
2017年 | 4篇 |
2016年 | 8篇 |
2015年 | 7篇 |
2014年 | 16篇 |
2013年 | 26篇 |
2012年 | 6篇 |
2011年 | 15篇 |
2010年 | 17篇 |
2009年 | 13篇 |
2008年 | 16篇 |
2007年 | 13篇 |
2006年 | 10篇 |
2005年 | 13篇 |
2004年 | 16篇 |
2003年 | 14篇 |
2002年 | 20篇 |
2001年 | 18篇 |
2000年 | 14篇 |
1999年 | 14篇 |
1998年 | 8篇 |
1997年 | 18篇 |
1996年 | 8篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 8篇 |
1992年 | 19篇 |
1991年 | 17篇 |
1990年 | 17篇 |
1989年 | 13篇 |
1988年 | 19篇 |
1987年 | 9篇 |
1986年 | 12篇 |
1985年 | 9篇 |
1984年 | 15篇 |
1983年 | 9篇 |
1982年 | 3篇 |
1980年 | 3篇 |
1979年 | 13篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1970年 | 3篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1932年 | 2篇 |
1922年 | 2篇 |
排序方式: 共有551条查询结果,搜索用时 0 毫秒
1.
2.
3.
4.
5.
6.
7.
George W. Haughn Julie Smith Barbara Mazur Chris Somerville 《Molecular & general genetics : MGG》1988,211(2):266-271
Summary A gene encoding acetolactate synthase was cloned from a chlorsulfuron-resistant mutant of Arabidopsis. The DNA sequence of the mutant gene differed from that of the wild type by a single base pair substitution. When introduced into tobacco by Ti plasmid-mediated transformation the gene conferred a high level of herbicide resistance. These results suggest that the cloned gene may confer agronomically useful levels of herbicide resistnace in other crop species, and that it may be useful as a selectable marker for plant transformation experiments. 相似文献
8.
9.
10.
Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II 总被引:19,自引:3,他引:16 下载免费PDF全文
A collection of 17 monoclonal antibodies elicited against the light-harvesting chlorophyll a/b protein complex which serves photosystem II (LHC-II) of Pisum sativum shows six classes of binding specificity. Antibodies of two of the classes recognize a single polypeptide (the 28- or the 26- kD polypeptides), thereby suggesting that the two proteins are not derived from a common precursor. Other classes of antibodies cross-react with several polypeptides of LHC-II or with polypeptides of both LHC-II and the light-harvesting chlorophyll a/b polypeptides of photosystem I (LHC-I), indicating that there are structural similarities among the polypeptides of LHC-II and LHC-I. The evidence for protein processing by which the 26-, 25.5-, and 24.5-kD polypeptides are derived from a common precursor polypeptide is discussed. Binding studies using antibodies specific for individual LHC-II polypeptides were used to quantify the number of antigenic polypeptides in the thylakoid membrane. 27 copies of the 26-kD polypeptide and two copies of the 28-kD polypeptide were found per 400 chlorophylls. In the chlorina f2 mutant of barley, and in intermittent light-treated barley seedlings, the amount of the 26-kD polypeptide in the thylakoid membranes was greatly reduced, while the amount of 28-kD polypeptide was apparently not affected. We propose that stable insertion and assembly of the 28-kD polypeptide, unlike the 26-kD polypeptide, is not regulated by the presence of chlorophyll b. 相似文献