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1.
E S Revazova Iu N Solov'ev T M Khizhniakova T V Iudicheva 《Biulleten' eksperimental'no? biologii i meditsiny》1985,99(4):469-471
The authors describe human cancer strains established from 6 cell lines transplanted to nude mice and rats: cancer of the liver, colon, lung, bladder and Burkitt's lymphoma. In spite of a long history (for several years) of cell line transfers in vitro, on transplantation of tumor cells, nude animals established tumors histologically identical to the primary cancer. 相似文献
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A Sh Byshevski? G S Solov'ev S L Galian V G Solov'ev S V Solov'ev 《Biulleten' eksperimental'no? biologii i meditsiny》1992,114(9):262-265
In experiment on white rats it was shown that preventive 12-day administration of vitamins A, E, C, P decreases the death rate of animals with exogenous thromboplastinemia and reduces hemocoagulative changes, microcirculation disorders, destructive changes of functionally active elements of inner organs. The protective effect of the above vitamins combination in thromboplastinemia is promoted by hypoactivity of platelet aggregation, by low thromboplastic activity of erythrocytes, by limited destruction of vascular endothelium. 相似文献
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M A Zenkova G G Karpova A S Levina S V Mamaev V V Solov'ev 《Bioorganicheskaia khimiia》1990,16(6):788-800
By site-directed alkylation of 16S rRNA with benzylidene derivatives of d(pACCTTGTT)rA (II), d(pTTACGACT)rU (III), d(pTTTGCTCCCC)rA (IV) (reagents (II)--(IV] followed by the RNase H treatment a number of 16S rRNA fragments have been obtained. Hybridisation of these fragments with restriction fragments of plasmid pKK 3535, containing operon rrnB of E. coli rRNAs, led to the identification of all reagents' binding sites in 16S rRNA. Good correlation is found between estimated stability of non-perfect 16S rRNA.oligodeoxyribonucleotide duplexes and the level of modification of this site with alkylating derivative of the same oligodeoxyribonucleotide. With high concentration of the reagents (II)--(IV) ((2-5) x 10(-5) M) the site-directed alkylation proceeds not only at the desired site but also at other sites corresponding to non-perfect duplexes between 16S rRNA and the reagents. It should be noted that the modification mainly occurs in the non-perfect duplexes, carrying mismatched bases at the termini. Influence of the secondary structure of 16S rRNA on the site-directed modification is discussed. 相似文献
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V N Solov'ev 《Antibiotiki》1967,12(9):857-861
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We studied UV-induced photodestruction of the native forms of bacteriochlorophyll a (Bchl a) from chromatophores and light harvesting complexes (LHC) of the sulphur photosynthetic bacterium Chromatium minutissimum. Irradiation of chromato- phores with 365-nm light (Soret band) or 280-nm light (absorption region of aromatic amino acids) led to the destruction of all long-wavelength forms of Bchl a. The quantum yields of photodestruction produced by the 280-nm light was higher than that produced by the 365-nm light. For the spectral forms of Bchl a absorbing at 850 nm and 890 nm, the difference was about one order of magnitude, and for the form absorbing at 800 nm the difference was almost two orders of magnitude. Similar UV sensitivity was observed for the Bchl a forms from isolated LHC. As a rule, the quantum yields of photodestruction induced by UV irradiation at 280 nm were about 100-1000 times higher (approximately 10(-3)-10(-4)) than that upon red light irradiation (approximately 10(-6)-10(-7)). We found that irradiation of chromatophores at 280 nm resulted in a crosslink between the core and peripheral LHC. 相似文献
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