Simplified and efficient DNA extraction protocol for Meliolaceae specimens

Meliolaceae is an obligate biotrophic fungal family which cannot be cultured in artificial media. This has resulted in a lack of DNA sequence data in public databases to better resolve species taxonomy. The main criterion for specific classification, therefore, relied heavily on host association. Fresh collections from living leaves of Croton persimilis and Tamarindus indica with black colonies in Mae Fah Luang University, Thailand yielded a new species, Irenopsis crotonicola sp. nov., and a new record of Meliola tamarindi. These taxa are described and phenotypic comparisons are made with known species. To better classify the putative novel species that cannot be cultivated, we outline the protocol to extract DNA from fruiting bodies and generate new phylogenetic data. The results indicate that this direct DNA extraction method is suitable to yield quality DNA sufficient for polymerase chain reaction (PCR) amplifications of several commonly used DNA regions in fungal systematics. The 28S rDNA phylogram generated confirms the position of our taxa within Meliolaceae and indicate a close relationship of I. crotonicola sp. nov. to I. walsurae. Sequences of tef, β-tubulin, and GPDH regions of Meliolaceae are provided as well.     

Unravelling evolutionary relationships between epifoliar Meliolaceae and angiosperms

Epifoliar fungi are a group of poorly studied fungal symbionts that coinhabit the surface of living plants. Meliolaceae is the largest group of epifoliar fungi and has been considered as obligate parasites. We investigated the taxonomy of Meliolaceae and the coevolutionary events with their host plants using time-calibrated cophylogeny based on large subunit, small subunit, and internal transcribed spacer (ITS) sequence data obtained from 17 different fungal taxa and rbcL, ITS, and trnH-psbA sequence data from their corresponding hosts. Nine new fungal species are introduced in this paper and Appendiculella is synonymized under Asteridiella. The dominant coevolutionary events during the Cretaceous and Cenozoic are cospeciation and host shift, respectively. We hypothesize that the evolutionary history of epifoliar fungi can be divided into three major periods: origins of families, formations of genera, and diversification of species. The rise of angiosperms prompted the evolution of modern epifoliar fungi and the diversification of orders of Angiospermae fostered the formation of epifoliar fungal genera. Phylogenetically, epifoliar fungal genera can be delimited according to their coevolutionary patterns and divergent periods.     

Comparison of water level and eutrophication indicators during the wet and dry period in a eutrophic urban lake

Eutrophication modifies lakes' ecological balances and threatens its viability. To date, eutrophication management strategies have been related to nutrient reduction in the lakes' water column. However, nutrient reduction strategies are complicated by the variations of the lake's water level, nutrient concentration, and eutrophication symptom, which are primarily known to be influenced by the local rainfall patterns. Therefore, this study aimed to compare the variability of water level, total phosphorus, and total chlorophyll-a concentrations in Slim River Lake during wet and dry seasons. In this study, water sampling and depth measurements were carried out from six sampling points for 1 year. Water samples were used to quantify total phosphorus and total chlorophyll-a. Our results showed that mean water levels in the studied lake ranged from 1.36 m to 5.46 m in the wet season and from 1.31 m to 5.41 m in the dry season, which implicated no significant difference (p > .05) between seasons in most sampling points. Total phosphorus present at concentrations exceeding 10 mg/L and showed small variations between wet and dry seasons. Mean total phosphorus concentrations varied from 10.55 mg/L to 26.66 mg/L in the wet season and 10.77 mg/L to 21.76 mg/L in the dry season and showed no significant difference between seasons. In addition, mean chlorophyll-a concentrations ranged from 14.35 mg/m³ to 180.13 mg/m³ and from 14.15 mg/m³ to 39.27 mg/m³ in wet and dry seasons, respectively. Chlorophyll-a concentrations showed significant differences (p < .05) between seasons in the deepest sampling points in the lake. The observed seasonal variations in total chlorophyll-a suggest the importance of algae monitoring during the wet season even when no apparent surge of phosphorus concentration is detected.