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Leaf-rust resistance and androgenesis were studied in the anther cultures of Triticum aestivum L., which included Saratovskaya 29 cultivar, the isogenic line Ps29, and three F1 hybrids (L503/S55, L504/S58, ATS7/L1063) with 7DS-7DL-7Ae#1L translocation of Lr19 gene (Lr19 translocation) from Agropyron elongatum (Host) P.B. The Lr19 translocation was shown to affect the induction of embryogenesis and green plant regeneration. The frequencies of Lr19 translocation differed in F2 hybrids obtained by traditional hybridization and in sets of DH lines obtained in F1 anther cultures derived from the same combinations of T. aestivum parental forms. The number of leaf-rust resistant genotypes tended to decrease. The frequency of Lr19 translocation in the set of DH3 lines derived from F1 L504/S58 was significantly lower than in other sets of DH3 lines and F2 hybrid populations. 相似文献
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Transmission of chromosomes 5R via gametes and its effect on somatic embryoidogenesis have been studied with the use of the model 5R(5A) substitution line L2837 = L503/Secale cereale L., cultivar Saratovskaya 5//L503, where L503 is a cultivar of spring bread wheat. It has been found that the frequencies of transmission of univalent chromosomes 5R and 5A determined in experiments on F1 reciprocal hybrids with cultivar Saratovskaya 29 do not reflect their frequencies in the self-pollinated offspring of F1 hybrids; the frequency of transmission of chromosomes 5R and 5A depends on the genotypes of both the recipient cultivar and the donor rye cultivar; and the 5R(5A) substitution in cultivar L503 significantly increases the parameters of somatic embryoidogenesis in vitro in explants from inflorescences. 相似文献
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Leaf-rust resistance and androgenesis were studied in the anther cultures of Triticum aestivum L., which included Saratovskaya 29 cultivar, the isogenic line Ps29, and three F1 hybrids (L503/S55, L504/S58, ATS7/L1063) with 7DS-7DL-7Ae#1L translocation of Lr19 gene (Lr19 translocation) from Agropyron elongatum (Host.) P.B. The Lr19 translocation was shown to affect the induction of embryogenesis and green plant regeneration. The frequencies of Lr19 translocation differed in F2 hybrids obtained by traditional hybridization and in sets of DH3 lines obtained in F1 anther cultures derived from the same combinations of T. aestivum parental forms. The number of leaf-rust resistant genotypes tended to decrease. The frequency of Lr19 translocation in the set of DH3 lines derived from F1 L504/S58 was significantly lower than in other sets of DH3 lines and F2 hybrid populations. 相似文献
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Yu E. Sibikeeva S. N. Sibikeev 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(6):782-785
A bread wheat cultivar, Saratovskaya 29, (S29), its nearly isogenic lines carrying alien translocations [Lr9 from Aegilops umbellulata (Eg29) and (Lr19) from Agropyron elongatum (Ps29)] and two F1 hybrids between three nearly isogenic lines of S29 that differed by the Lr19+Rht1,Pro1+Pro2 and Ppd1+Ppd2 gene complexes, namely the S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) F1 and the S29 (Pro1+Pro2)/S29 (Lr19+Rht1) F1 were studied for their culture response with the following results. (1) Translocations with Lr9 and Lr19 decreased embryo frequency and green plant regeneration. (2) Both F1 hybrids showed a decrease in embryo frequency. One of the F1 hybrids, S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) showed a decrease, with respect to S29 for green plant regeneration; the other F1 S29 (Pro1+Pro2)/S29 (Lr19+Rht1), equalled S29 for green plant regeneration. (3) The gene complex of the F1 hybrid S29 (Pro1+Pro2)/S29 (Lr19+Rht1) was better than that of the F1 hybrid S29 (Lr19+Rht1)/S29 (Ppd1+Ppd2) for embryo induction and green plant regeneration. This effect was possibly induced by interactions between the Pro1+Pro2 and Lr19+Rht1 genes or was the result of direct actions of the Pro1+Pro2 genes. 相似文献
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Transmission of chromosomes 5R and 5A via gametes and its effect on somatic embryoidogenesis have been studied with the use of the model 5R(5A) substitution line L2837 = L503/Secale cereale L., cultivar Saratovskaya 5/L503, where L503 is a cultivar of spring bread wheat. It has been found that the frequencies of transmission of univalent chromosomes 5R and 5A determined in experiments on F1 reciprocal hybrids with cultivar Saratovskaya 29 do not reflect their frequencies in the self-pollinated offspring of F1 hybrids; the frequency of transmission of chromosomes 5R and 5A depends on the genotypes of both the recipient cultivar and the donor rye cultivar; and the 5R(5A) substitution in cultivar L503 significantly increases the parameters of somatic embryoidogenesis in vitro in explants from inflorescences. 相似文献
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The features of in vitro androgenesis were studied in cultured anthers of spring bread wheats L503 and Dobrynya, having 7DS-7DL-7Ae#1L translocation with genes Lr19/Sr25 (Lr19 translocation) from Agropyron elongatum (Host.) P.B. and their near-isogenic lines carrying combinations of Lr19 translocation with translocations: 1BL-1R#1S with genes Pm8/Sr31/Lr26/Yr9 (Lr26 translocation) from Secale cereale L., 4BS-4BL-2R#1L with genes Lr25/Pm7 (Lr25 translocation) from Secale cereale, 3DS-3DL-3Ae#lL with genes Lr24/Sr24 (Lr24 translocation) from Agropyron elongatum and 6BS-6BL-6U#1L with gene Lr9 (Lr9 translocation) from Aegilops umbellulata Zhuk. In comparison with those varieties having received the Lr19 translocation, the following was established: (1) the combination of translocations Lr19 + 26 increased embryo frequency and green plant regeneration; (2) the combination of translocations Lr19 + 9 decreased embryo frequency but increased green plant regeneration; (3) the combination of translocations Lr19 + 24 decreased embryo frequency but increased green and albino plant regeneration; (4) the combination of translocations Lr19 + 25 increased embryo frequency and green plant regeneration but decreased albino plant regeneration. Thus, on near-isogenic lines of spring bread wheat, the influences of genotypes of four alien translocation combinations on in vitro androgenesis were determined. 相似文献
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