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排序方式: 共有171条查询结果,搜索用时 31 毫秒
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Tong Wu Qi Tian Ruiji Liu Ke Xu Shanshan Shi Xiudi Zhang Liming Gao Xiaobo Yin Shufeng Xu Ping Wang 《Journal of cellular and molecular medicine》2023,27(22):3526-3538
Exosomal microRNA (miRNA) exerts potential roles in non-small-cell lung cancer (NSCLC). The current study elucidated the role of miR-30b-5p shuttled by bone marrow mesenchymal stem cells (BMSCs)-derived exosomes in treating NSCLC. Bioinformatics analysis was performed with NSCLC-related miRNA microarray GSE169587 and mRNA data GSE74706 obtained for collection of the differentially expressed miRNAs and mRNAs. The relationship between miR-30b-5p and EZH2 was predicted and confirmed. Exosomes were isolated from BMSCs and identified. BMSCs-derived exosomes overexpressing miR-30b-5p were used to establish subcutaneous tumorigenesis models to study the effects of miR-30b-5p, EZH2 and PI3K/AKT signalling pathway on tumour growth. A total of 86 BMSC-exo-miRNAs were differentially expressed in NSCLC. Bioinfomatics analysis found that BMSC-exo-miR-30b-5p could regulate NSCLC progression by targeting EZH2, which was verified by in vitro cell experiments. Besides, the target genes of miR-30b-5p were enriched in PI3K/AKT signalling pathway. Animal experiments validated that BMSC-exo-miR-30b-5p promoted NSCLC cell apoptosis and prevented tumorigenesis in nude mice via EZH2/PI3K/AKT axis. Collectively, the inhibitory role of BMSC-derived exosomes-loaded miR-30b-5p in NSCLC was achieved through blocking the EZH2/PI3K/AKT axis. 相似文献
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不同时期开沟施氮对水稻物质生产及产量的影响 总被引:2,自引:1,他引:2
通过田间试验 ,研究了水稻不同生育时期开沟深施氮肥对水稻叶片、叶鞘和茎秆干重以及生物产量和籽粒产量的影响 .结果表明 ,孕穗期开沟深施氮肥处理比分蘖期开沟、穗分化始期开沟和不开沟处理的水稻叶片干重保持最大值 (2 .9g/穴 )时间长 ,叶面积指数达到最大值 (LAI =8.9)后保持缓慢下降 ;叶鞘干重 (2 .7g/穴 )变化小 ;处理以后茎秆干重 (4.3g/穴 )稳步增加 .孕穗期开沟施肥处理的水稻生物产量(0 .73g·d-1/穴 )递增速度快 ,籽粒产量 (10 4 34kg·hm-2 )高 .与不开沟施肥相比 ,孕穗期开沟施氮对产量增加作用最大 ,为水稻开沟深施氮肥的最佳时期 ;其次为穗分化始期 ,分蘖期开沟施氮效果较差 ,但仍有一定的增产作用 . 相似文献
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Kaidong Ma Hong Jin Rong Hu Yu Xiong Shufeng Zhou Peng Ting Yan Cheng Yan Yang Pengyuan Yang Xiaotian Li 《Cell biochemistry and biophysics》2014,69(2):247-258
To explore the proteomic changes of placental trophoblastic cells in preeclampsia–eclampsia (PE), placental trophoblastic cells from normally pregnant women and women with hypertension during gestational period were prepared by laser capture microdissection (LCM), and proteins isolated from these cells were subjected to labeling and proteolysis with isotope-coded affinity tag reagent. A qualitative and quantitative analysis of the proteome expression of placental trophoblastic cells was made using two-dimensional liquid chromatography tandem mass spectrometry (2D LC–MS/MS). A total of 831 proteins in placental trophoblastic cells were identified by combined use of LCM technique and 2D LC–MS/MS. The result was superior to that of conventional two-dimensional electrophoresis method. There were marked differences in 169 proteins of placental trophoblastic cells between normally pregnant women and women with PE. Of 70 (41.4 %) proteins with more than twofold differences, 31 proteins were down-regulated, and 39 were up-regulated in placental trophoblastic cells of the woman with PE. Laminin expression in placenta trophoblastic cells of women with PE was significantly down-regulated as confirmed by Western blot analysis. These findings provide insights into the proteomic changes in placental trophoblastic cells in response to PE and may identify novel protein targets associated with the pathogenesis of PE. 相似文献
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本研究通过体外生化实验研究细胞色素P450 3A7对维生素D3的羟化作用。根据GenBank报道的序列设计特异引物,扩增cyp3a7的编码区,将cyp3a7的编码区插入到pcDNATM3.1/myc-His(-) A的XhoⅠ/Bam HⅠ,通过测序检测序列的正确性。pcDNA-CYP3A7及pcDNA分别瞬时转染293T细胞,48 h后收集细胞,提取S9组分,用Bradford法测定蛋白质浓度。S9组分经12%SDS-PAGE凝胶电泳和Western blotting检测,用myc抗体作为一抗检测CYP3A7在293T细胞的表达水平。0.6 mg S9组分与1μmol/L维生素D3于37℃孵育30 min,用4倍体积的氯仿甲醇(体积比为3∶1)抽提,有机相在氮气流下吹干,残基用于HPLC分析。结果显示,重组表达CYP3A7的293T细胞的S9组分通过Western blotting检测到了特异的约60 kD的条带,对照样品未检测到特异条带的蛋白质。重组表达CYP3A7的293T细胞S9组分的孵育样品通过HPLC检测到了25-羟基维生素D3,对照样品未检测到25-羟基维生素D3。结果表明重组表达的CYP3A7羟化维生素D3生成25-羟基维生素D3。本研究为进一步探究还有哪些P450参与维生素D3在鸡体内的代谢,为阐明其代谢途径提供理论依据。 相似文献
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Jun Liu Junchen Chen Yadan Zhong Xiaoling Yu Ping Lu Jianqi Feng Xin Zhang Shufeng Ma Chao Yang Bin Yang Zhili Rong 《蛋白质与细胞》2021,12(8):653-660
Dear Editor,
Primary localized cutaneous amyloidosis (PLCA) is a skin-limited disorder characterized by deposition of amyloid material in the superficial dermis.According to clinical characteristics,PLCA is divided into lichen,macular,and nodular amyloidosis.PLCA is found worldwide but has a higher incidence in South America and Southeast Asia,such as in Brazil and China (Chang et al.,2014;Tey et al.,2016). 相似文献
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