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1.
Histochemical reaction with glyoxylic acid has revealed a specific fluorescence caused by the presence of indolamine (apparently serotonin) in the nervous system of larva and mature cestode. Fluorescence manifests itself in neurons and nerve fibres of the central ganglion and its commissure, in nerve cells of the proboscis, in longitudinal trunks and transverse commissures, and in the nerve elements connected with genital system. 相似文献
2.
E B Moroshkina A K Shishov M A Krivtsova N N Zhadin E V Frisman 《Molekuliarnaia biologiia》1975,9(6):836-844
DNA--acriflavin complexes have been investigated by the methods of flow birefringence and viscometry. The intrinsic viscosity and the optic anysotropy of the complex increase with the increasing quantities of binding dye. Experimental data are treated on the basis of different models of binding. At high ionic strength (mu = 0,1) one type of binding takes place which is described by the intercalation model. In this case the thermodynamic rigidity of DNA-molecule within the complex is proportional to "r". In solutions of low ionic strength (mu = 0,001), two types of DNA-acriflavin binding occur: intercalation and external binding. At low ionic strength, the spectrophotometric titration technique is shown to give a reduced value of "r". 相似文献
3.
Malikina KD Shishov VA Chuvelev DI Kudrin VS Oleskin AV 《Prikladnaia biokhimiia i mikrobiologiia》2010,46(6):672-677
Proliferation of Saccharomyces cerevisiae EPF cells on solid maltose-peptone-yeast extract (MPY) medium was stimulated by the addition of monoamine neurotransmitters. Dopamine turned out to be the most efficient among them: it caused approximately 8-fold growth stimulation at 1 microM concentration. The dopamine effect was partly mimicked by apomorphine, a dopamine receptor agonist. Serotonin and histamine produced less significant (1.5-2-fold) effects, and norepinephrine virtually failed to stimulate yeast culture growth. These data point to a specific, apparently receptor-dependent mode of action of the tested neurotransmitters on S. cerevisiae cells. Using high efficiency liquid chromatography, serotonin, catecholamines (dopamine and norepinephrine), catecholamines precursor dioxyphenylamine, and oxidized amine products (homovanilic acid, dihydrophenylacetic acid, and 5-hydroxyindolacetic acid) were established to be accumulated in yeast cells up to (sub)micromolar concentrations without their release into the culture fluid supernatant (CSF). The results obtained suggest that the tested amine neurotransmitters and related compounds do not serve as autoregulators in the yeast population. Nevertheless, they may be involved in the regulation of yeast population development by other ecosystem components. 相似文献
4.
5.
Iu K Smirnov A S Shishov N N Mal'tseva 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1978,(10):58-61
Changes of antibody formation of 96 patients with various clinical forms of herpes zoster were traced by indirect hemagglutination inhibition test. Four variants of the immunological response were distinguished; clinical characteristics of each of these groups is presented. The initial antibody level failed to determine the severity of the course of the disease in herpes zoster. 相似文献
6.
V. A. Shishov T. A. Kirovskaya V. S. Kudrin A. V. Oleskin 《Applied Biochemistry and Microbiology》2009,45(5):494-497
The growth dynamics of the synthesis of monoamine neuromediators serotonin, norepinephrine, and dopamine in Escherichia coli K-12 was investigated for the first time using high performance liquid chromatography with electrodetection. Maximum (micromolar)
concentrations of these compounds were detected in E. coli cells during the early growth phases; their intracellular content decreases after the transition to late growth phases. E. coli biomass contains (i) the substances DOPA and 5-hydroxytryptamine that serve in animal cells as neuromediator precursors and
(ii) the products of their oxidative deamination. Presumably, the biosynthesis and degradation of monoamine neuromediators
in bacterial cells involves enzyme systems analogous to those typical of animals. The culture fluid of E. coli contains micromolar concentrations of DOPA and nanomolar of serotonin, dopamine, and norepinephrine during the late growth
phase. These concentrations are sufficient for animal/human receptors to bind them. This article deals with the potential
biotechnological applications of the data obtained. 相似文献
7.
K. D. Malikina V. A. Shishov D. I. Chuvelev V. S. Kudrin A. V. Oleskin 《Applied Biochemistry and Microbiology》2010,46(6):620-625
Proliferation of Saccharomyces cerevisiae EPF cells on solid maltose-peptone-yeast extract (MPY) medium was stimulated by the addition of monoamine neurotransmitters.
Dopamine turned out to be the most efficient among them: it caused ∼8-fold growth stimulation at 1 μM concentration. The dopamine
effect was partly mimicked by apomorphine, a dopamine receptor agonist. Serotonin and histamine produced less significant
(1.5–2-fold) effects, and norepinephrine virtually failed to stimulate yeast culture growth. These data point to a specific,
apparently receptor-dependent mode of action of the tested neurotransmitters on S. cerevisiae cells. Using high performance liquid chromatography, serotonin, catecholamines (dopamine and norepinephrine), catecholamine
precursor dioxyphenylamine, and oxidized amine products (homovanilic acid, dihydrophenylacetic acid, and 5-hydroxyindolacetic
acid) were established to be accumulated in yeast cells up to (sub)micromolar concentrations without their release into the
culture fluid supernatant (CFS). The results obtained suggest that the tested amine neurotransmitters and related compounds
do not serve as autoregulators in the yeast population. Nevertheless, they may be involved in the regulation of yeast population
development by other ecosystem components. 相似文献
8.
I N Shishov V G Ma?ski? G I Basilova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1986,(7):37-40
The assimilation and mutual transformation of exogenous purine and pyrimidine bases and their nucleosides in the known subspecies of F. tularensis have been studied by means of radio-labeled compounds. The possibility of using the specific features of the metabolism of these compounds in F. tularensis, established in this study, for taxonomy and differential diagnosis has been demonstrated. 相似文献
9.
Aruna Kasoju M Lakshmi Narasu Charuvaka Muvva Bathula VV SubbaRao 《Bioinformation》2012,8(14):684-686
Aflatoxins are polyketide-derived secondary metabolites produced by Aspergillus spp. The toxic effects of aflatoxins have adverse
consequences for human health and agricultural economics. The aflR gene, a regulatory gene for aflatoxin biosynthesis, encodes a
protein containing a zinc-finger DNA-binding motif. AFLR-Protein three-dimensional model was generated using Robetta server.
The modeled AFLR-Protein was further optimization and validation using Rampage. In the simulations, we monitored the
backbone atoms and the C-α-helix of the modeled protein. The low RMSD and the simulation time indicate that, as expected, the
3D structural model of AFLR-protein represents a stable folding conformation. This study paves the way for generating computer
molecular models for proteins whose crystal structures are not available and which would aid in detailed molecular mechanism of
inhibition of aflatoxin. 相似文献
10.
Visualization of molecular structures aids in the understanding of structural and functional roles of biological macromolecules.
Macromolecular transport between the cell nucleus and cytoplasm is facilitated by the nuclear pore complex (NPC). The ring
structure of the NPC is large and contains several distinct proteins (nucleoporins) which function as a selective gate for the passage
of certain molecules into and out of the nucleus. In this note we demonstrate the utility of a python code that allows direct
mapping of the physiochemical properties of the constituent nucleoporins on the scaffold of the yeast NPC׳s cytoplasmic view. We
expect this tool to be useful for researchers to visualize the NPC based on their physiochemical properties and how it alters when
specific mutations are introduced in one or more of the nucleoporins. The code developed using Python is available freely from the
authors. 相似文献