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Caulobacter crescentus flagellar filaments were examined for their shape and handedness. Contour length, wavelength and height of the helical filaments were 1.34 +/- 0.14 micron, 1.08 +/- 0.05 micron and 0.27 +/- 0.04 micron, respectively. Together with the value of the filament diameter, 14 +/- 1.5 nm, the parameters of the curvature (alpha) and twist (phi) were calculated as 3.9(%) for alpha and 0.026 (rad) for phi, which are similar to those of the curly I filament of Salmonella typhimurium. Dark-field light microscopic analysis revealed that the C. crescentus wild-type filament possesses a right-handed helical form. Given the result that C. crescentus cells normally swim forward, in the opposite direction to a polar flagellum, it is likely that C. crescentus swims by rotation of a right-handed curly shaped flagellum in a clockwise sense, whereas S. typhimurium and Escherichia coli swim by rotation of left-handed normal type flagella in a counterclockwise sense. 相似文献
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Hideyoshi Yoshida Miki Shirakihara Akira Takemura Kunio Shirakihara 《Marine Mammal Science》1994,10(3):266-282
Development, sexual dimorphism, and individual variation were examined in the skeleton of the finless porpoise in the coastal waters of western Kyushu, Japan. Skulls ceased growing by 4 yr. Postcranial skeletons ceased increasing in size at an age older than 11 yr. The finless porpoise was estimated to attain cranial maturity by 4 yr and physical maturity at 14–23 yr. Sexual dimorphism was not detectable in most of the cranial characters but was detected in more than half of the postcranial characters. Females tended to show larger values of postcranial characters. The shape of the pelvic bone was obviously different between males and females. Thus, a discriminant function was proposed to determine sex using measurements of this bone. Individual variation was greatest in the feeding apparatus such as length of the rostrum, and least in the braincase. 相似文献
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AGE, GROWTH, AND REPRODUCTION OF THE FINLESS PORPOISE, NEOPHOCAENA PHOCAENOIDES, IN THE COASTAL WATERS OF WESTERN KYUSHU, JAPAN 总被引:1,自引:0,他引:1
Abstract: In the coastal waters of western Kyushu, Japan, a total of 97 incidentally taken or stranded finless porpoises, Neophocaena phocaenoides , was collected for studying age, growth and reproduction. An additional 17 specimens from the Inland Sea were used for a comparison of life history. Mean neonatal body length was 78.2 cm. Both males and females grew to around 140 cm by 5 yr of age. The maximum body lengths of males and females in western Kyushu were 174.5 cm and 165.0 cm, respectively, which were smaller than those recorded in other Japanese waters. Females probably attain sexual maturity at ages of 6–9 yr and at body lengths of 135–145 cm. Males probably mature sexually at ages of 4–6 yr, at body lengths of 135–140 cm and at weight of testis of 40–150 g. The lack of females aged 5–6 yr and males aged 4–5 yr precluded firm conclusions on ages at sexual maturity. Parturition in western Kyushu was estimated to be prolonged from autumn to spring, whereas in the Inland Sea and Pacific waters it was restricted from spring to summer with a peak in April. These geographical differences and available information on distribution implies that the finless porpoises in western Kyushu constitute a local population. 相似文献
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Miki Shirakihara Kunio Shirakihara Jyunji Tomonaga Megumi Takatsuki 《Marine Mammal Science》2002,18(1):30-41
Residency patterns and population size were examined for Indo-Pacific bottlenose dolphins ( Tursiops aduncus ) in the coastal waters of Amakusa-Shimoshima Island, western Kyushu, Japan, from summer 1994 to fall 1998. A total of 203 individuals were identified. The number of newly identified individuals barely increased after the first 1.5 yr. The identified individuals in one season were frequently resighted with percentages of mostly over 60% during the subsequent seasons. Most of the dolphins off Amakusa were yearround residents, although the total range of their habitat was unknown. The population size estimated by the mark-recapture technique was 218 individuals with a CV of 5.41% in 1995–1997. The group commonly consisted of more than 100 individuals. The large group size was thought to be a response to feeding on schooling fishes. 相似文献
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We report the crystal structure of an enolase from Enterococcus hirae, which is the first report of a structure determination among gram-positive bacteria. We isolated the enolase gene and determined the base sequence. The amino acid sequence deduced from the DNA sequence suggests that this enolase is composed of 431 amino acids. The amino acid sequence is very similar to those of enolases from eukaryotic and prokaryotic organisms, being 65% and 50% identical to enolases from Escherichia coli and yeast, respectively. The enolase prepared from E. hirae lysate yielded crystals containing one dimer per asymmetric unit. X-ray diffraction patterns were obtained at 2.8 A resolution on a SPring-8 synchrotron radiation source. Crystals belong to space group I4 with unit cell dimensions of a = b = 153.5 A, c = 90.7 A. The E. hirae, yeast, E. coli and lobster enolase structures are very similar. The E. hirae enolase takes an "Open" conformation. The regions in the structure that differ most from other enolases are loops L4 (132-140) and L3 (244-265). Considering the positions of these loops relative to the active site, they seem to have no direct involvement in function. Our findings show that the three dimensional structure of an important enzyme in the glycolytic pathway is evolutionarily conserved among eukaryotes and prokaryotes, including gram-positive bacteria. 相似文献
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Two species competition model is built up by assuming the hypothetical second order interactions in order to consider effects of exploitation on two competing fish species with non-linear interactions. Most important characteristic of this model, compared withLotka-Volterra type linear competition model, is that this model can possess multiple stable equilibrium points. Therefore there is a possibility that two species keeping the equilibrium state at one stable equilibrium point will be attracted to the other stable equilibrium point after a heavy perturbation. In this model reversible change of the fishing pressure does not always results in that of the equilibrium catch. In this sence MSY concept for single species can not be extended to this model. If there are multiple stable equilibrium points, the change of the dominant fish species, catastrophic and irreversible change of each equilibrium catch may be observed when the perturbation by the exploitation is added. This phenomenon immediately reminds us of the change of the dominant fish species between Japanese common mackerel and Pacific saury in the northwest Pacific Ocean. In case of the management of two competing fish species with nonlinear interactions, the consideration on the balance between the fishing pressure for each species may be as important as the decision on the catch limit for each species. MSY level for each species based on the single-species theory could be quite erroneous. 相似文献