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Summary The antibacterial effect of negapillin in combination with other antibiotics and sulfa drugs was studied using Gram+ve and Gram-ve bacteria as test organisms. Subsequently, various proportions of negapillin in combination with the antibiotics and the sulfa drugs were examined to determine the optimum proportions which exert maximum inhibitory effects on the bacterial growth. Mixtures of these antibiotics with negapillin were found more superior not only as antimicrobial agents but also for having comparatively low toxicity. 相似文献
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Summary The influence exerted by some cultural conditions on the ability of metabolites of Bacillus subtilis to liquefy the human blood clots was studied. Crude enzyme preparations capable of liquefying the clots were isolated from the culture solutions. The aminoacids produced during the liquefaction process could be traced. Both of the enzyme preparation and the metabolism solution induced no obvious toxic effects when injected into veins of rabbits. 相似文献
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Mark Kittisopikul Takeshi Shimi Meltem Tatli Joseph Riley Tran Yixian Zheng Ohad Medalia Khuloud Jaqaman Stephen A. Adam Robert D. Goldman 《The Journal of cell biology》2021,220(4)
Nuclear lamin isoforms form fibrous meshworks associated with nuclear pore complexes (NPCs). Using datasets prepared from subpixel and segmentation analyses of 3D–structured illumination microscopy images of WT and lamin isoform knockout mouse embryo fibroblasts, we determined with high precision the spatial association of NPCs with specific lamin isoform fibers. These relationships are retained in the enlarged lamin meshworks of Lmna−/− and Lmnb1−/− fibroblast nuclei. Cryo-ET observations reveal that the lamin filaments composing the fibers contact the nucleoplasmic ring of NPCs. Knockdown of the ring-associated nucleoporin ELYS induces NPC clusters that exclude lamin A/C fibers but include LB1 and LB2 fibers. Knockdown of the nucleoporin TPR or NUP153 alters the arrangement of lamin fibers and NPCs. Evidence that the number of NPCs is regulated by specific lamin isoforms is presented. Overall the results demonstrate that lamin isoforms and nucleoporins act together to maintain the normal organization of lamin meshworks and NPCs within the nuclear envelope. 相似文献
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Ibrahim R. Shimi Moustafa S. Nour El Dein Gamal M. Imam 《Archives of microbiology》1961,39(3):292-297
Summary The formation of fat from citric, succinic, fumaric and malic acids by Penicillium spinulosum has been investigated. Sodium arseite, -sulfonic propionic acid and mono-iodoacetate was found to control unfavourably the formation of fat from the organic acids by the preformed pellets of the organism. Moreover, iodoacetate reduced the mean molecular weight of the component fatty acids. The -sulfonic propionic acid though reduced the yields of fat yet the ratio fat/100 g of acid consumed was not markedly altered by this compound. A modified chromatographic method was described for the assay of different acids.With 3 Figures in the text 相似文献
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Multispectral imaging technologies have been widely used in fields of astronomy and remote sensing. Interdisciplinary approaches developed in, for example, the National Aeronautics and Space Administration (NASA, USA), the Jet Propulsion Laboratory (JPL, USA), or the Communications Research Laboratory (CRL, Japan) have extended the application areas of these technologies from planetary systems to cellular systems. Here we overview multispectral imaging systems that have been devised for microscope applications. We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering. 相似文献
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Takeshi Shimi Mark Kittisopikul Joseph Tran Anne E. Goldman Stephen A. Adam Yixian Zheng Khuloud Jaqaman Robert D. Goldman 《Molecular biology of the cell》2015,26(22):4075-4086
The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin A, C, B1, and B2 in mouse embryo fibroblast nuclei. Each isoform forms a distinct fiber meshwork, with comparable physical characteristics with respect to mesh edge length, mesh face area and shape, and edge connectivity to form faces. Some differences were found in face areas among isoforms due to variation in the edge lengths and number of edges per face, suggesting that each meshwork has somewhat unique assembly characteristics. In fibroblasts null for the expression of either lamins A/C or lamin B1, the remaining lamin meshworks are altered compared with the lamin meshworks in wild-type nuclei or nuclei lacking lamin B2. Nuclei lacking LA/C exhibit slightly enlarged meshwork faces and some shape changes, whereas LB1-deficient nuclei exhibit primarily a substantial increase in face area. These studies demonstrate that individual lamin isoforms assemble into complex networks within the nuclear lamina and that A- and B-type lamins have distinct roles in maintaining the organization of the nuclear lamina. 相似文献
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Biological breakdown of benzylpenicillin by preformed mats of penicillin-producing organisms
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1. Penicillium chrysogenum and Aspergillus flavus degraded benzylpenicillin in the same way. 2. Degradation of the antibiotic was initiated by the cleavage of phenylacetic acid from 6-aminopenicillanic acid. 3. Phenylacetic acid was left unchanged whereas 6-aminopenicillanic acid was degraded into cysteine and valine. This reaction is probably complex. 4. Cysteine was not utilized but valine was cleaved into acetone and glycine. Catabolism of valine by the preformed mats of the two moulds confirms this result. This step probably involves the formation of propan-2-ol. 5. Cleavage of benzylpenicillin into phenylacetic acid and 6-aminopenicillanic acid was performed through the activity of a cellular-bound enzyme. 相似文献
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