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排序方式: 共有423条查询结果,搜索用时 2 毫秒
1.
Cuticlin: a noncollagen structural protein from Ascaris cuticle 总被引:6,自引:0,他引:6
A structural protein was isolated from the cuticle of Ascaris lumbricoides and studied by chemical analyses, electron microscopy, and X-ray diffraction. It has high contents of proline and alanine and relatively low contents of glycine and basic amino acids. It does not give the characteristic wide-angle X-ray diffraction pattern of collagen. It is not susceptible to bacterial collagenase. In these respects, it is distinct from collagen. The name “cuticlin” is proposed for this protein. 相似文献
2.
In vitro binding of nuclear proteins from wheat germ to the5'-upstream region of the rolC gene of Ri plasmid was investigated.The specific DNA sequences interacting with proteins were detectedby DNase I footprinting. (Received October 8, 1990; Accepted November 30, 1990) 相似文献
3.
Yasushi Oda Haruki Nakamura Toshio Yamazaki Kuniaki Nagayama Mayumi Yoshida Shigenori Kanaya Morio Ikehara 《Journal of biomolecular NMR》1992,2(2):137-147
Summary Two-dimensional (2D)1H NMR experiments using deuterium labeling have been carried out to investigate the solution structure of ribonuclease HI (RNase HI) fromEscherichia coli (E. coli), which consists of 155 amino acids. To simplify the1H NMR spectra, two fully deuterated enzymes bearing several prototed amino acids were prepared from an RNase HI overproducing strain ofE. coli grown in an almost fully deuterated medium. One enzyme was selectively labeled by protonated His, He. Val. and Leu. The other was labeled by only protonated His and Ile. The 2D1H NMR spectra of these deuterated R Nase H1 proteins, selectively labeled with protonated amino acids, were much more simple than those of the normally protonated enzyme. The simplified spectra allowed unambiguous assignments of the resonance peaks and connectivities in COSY and NOESY for the side-chain protons. The spin-lattice relaxation times of the side-chain protons of the buried His residue of the deuterated enzyme became remarkably longer than that of the protonated enzyme. In contrast, the relaxation times of the side-chain protons of exposed His residues remained essentially unchanged. 相似文献
4.
Molecular cloning and nucleotide sequence of Thermus thermophilus HB8 trpE and trpG 总被引:11,自引:0,他引:11
The trpE gene of Thermus thermophilus HB8 was cloned by complementation of an Escherichia coli tryptophan auxotroph. The E. coli harboring the cloned gene produced the anthranilate synthase I, which was heat-stable and enzymatically active at higher temperature. The nucleotide sequence of the trpE gene and its flanking regions was determined. The trpE gene was preceded by an attenuator-like structure and followed by the trpG gene, with a short gap between them. No other gene essential for tryptophan biosynthesis was observed after the trpG gene. The amino-acid sequences of the T. themophilus anthranilate synthase I and II deduced from the nucleotide sequence were compared with those of other organisms. 相似文献
5.
Cyanogen bromide (BrCN) condensed oligoadenylates [oligo(A)] on a poly(uridylic acid) [poly(U)] template in an aqueous solution. Imidazole and divalent metal ions such as Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Mg2+, and Fe2+ were required for the condensation. Chain length of oligo(A) and reaction temperature affected the coupling yield. Hexaadenylate [(pA)6] was converted to (pA)12, (pA)18, (pA)24, (pA)30, (pA)36, (pA)42, and (pA)48 in a 68% overall yield for 20 h at 25 degrees C. The coupling yield increased with increase in the poly(U) concentration. Five- to sevenfold molar excess of uridylyl residues of poly(U) to adenylyl residues of oligo(A) gave the best yield (68%). Metal ions affected the formation of linkage isomers of the phosphate bonds: The 2',5'- and 3',5'-phosphodiester bonds were predominant in the presence of Co2+, Zn2+, and Ni2+ and the 5',5'-pyrophosphate bond was predominant in the presence of Mn2+. In particular, Ni2+ gave the highest ratio of the 3',5'-phosphodiester bond (30%). N-Cyanoimidazole (1), N,N'-iminodiimidazole (2), and N-carboxamidoimidazole (3) were formed in a reaction of imidazole with BrCN in an aqueous solution. 1 and 2 had much the same condensing activity for the polymerization of adenylates as BrCN. A reaction pathway was proposed in which 1 and 2 are not only intermediates for the production of 3 but also the true condensing agent in the coupling reaction of oligo(A). Phosphorimidazolide derivative was detected in a reaction of 5'-AMP with either 1 or 2. The condensation would proceed by way of N-cyanoimidazole-phosphate adduct, the phosphorimidazolide derivative, or both. 相似文献
6.
A model for the involvement of the small nucleolar RNA (U3) in processing eukaryotic ribosomal RNA 总被引:15,自引:0,他引:15
The nucleotide sequence of chick pre-rRNA between 5.8S and 28S rRNAs is 85% G + C and has the potential to form many different secondary structures. A model is presented in which a small nucleolar RNA, U3, and its associated proteins act as an RNA isomerase to position the pre-rRNA for processing. Cleavage could be performed either by a nuclease present in the U3RNP or by a ribonuclease directed to the proper form of the pre-rRNA. 相似文献
7.
N Kanaya 《Mutation research》1990,228(2):187-192
The induction of sister-chromatid exchanges (SCEs) and chromosome aberrations (CAs) by benzo[a]pyrene (BP), 2-aminofluorene (2-AF) and cyclophosphamide (CP) in the root cells of Vicia faba was examined. BP and 2-AF induced CAs, but not SCEs. CP induced both SCEs and CAs. 相似文献
8.
N Kanaya 《Mutation research》1990,245(4):311-315
The induction of sister-chromatid exchanges (SCEs) and chromosome aberrations (CAs) by aniline hydrochloride (AH) and its C-hydroxylated metabolites, o-, m- and p-aminophenol, in the root cells of Vicia faba was examined. AH induced CAs, but not SCEs. All the C-hydroxylated metabolites of aniline induced both SCEs and CAs. However, the treatment of cells with these metabolites at concentrations that did not cause significant increases in CAs resulted in significant increases in SCEs. These results seem to suggest that the substance that induced CAs in root cells treated with AH was not the C-hydroxylated metabolites of aniline. 相似文献
9.
Kazuyuki Morihara Tatsushi Oka Hiroshige Tsuzuki Yoshiharu Tochino Takashi Kanaya 《Biochemical and biophysical research communications》1980,92(2):396-402
We have established a procedure for converting porcine insulin into human insulin using a serine protease from M497-1 which shows unique specificity against lysine residues on the carboxyl side of the splitting point. Desalanine-(B30)-insulin (DAI) was prepared by digestion of porcine insulin with protease. The coupling between DAI and Thr-OBut was performed by the same enzyme at pH 6.5 with a large excess of the amine component (Thr-OBut) in the presence of high concentrations of organic co-solvents. The highest yield was 85% by 20 h reaction at 37°C. The synthesized [Thr-OBut-B30]-insulin was isolated, then deprotected with trifluoroacetic acid in the presence of anisole to obtain semisynthetic human insulin. 相似文献
10.
S Kimura Y Oda T Nakai K Katayanagi E Kitakuni C Nakai H Nakamura M Ikehara S Kanaya 《European journal of biochemistry》1992,206(2):337-343
The size of the cavity around Ser68 of Escherichia coli ribonuclease HI was modulated by amino acid substitutions to examine the effects on the stability of the enzyme. Five mutant proteins, Ser68----Gly, Ser68----Ala, Ser68----Thr, Ser68----Val and Ser68----Leu, were constructed. Each of the mutant proteins exhibited at least 40% of the enzyme activity of the wild-type protein. The stabilities of the mutant proteins were determined from urea-denaturation and thermal-denaturation curves. Among the five mutations, only the Ser----Val mutation resulted in an increase in the stability of the enzyme. The melting temperature, tm, at pH 3.0 of the mutant protein Ser68----Val was increased by 1.9 degrees C. Its free-energy change of unfolding in the absence of urea, delta G(H2O), and the midpoint of the denaturation curve, [D]1/2, were also increased by 5.4 kJ/mol and 0.18 M, respectively. The increase in the stability of the enzyme is probably due to the filling of the cavity space around Ser68 by valine. However, the mutation of Ser68 to glycine or leucine residues resulted in a considerable decrease in stability. In these cases, some conformational changes occur, as suggested by the CD and 1H-NMR spectra of these mutant proteins. 相似文献