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M A Pilinskaia M Kh Shigaeva G V Kasinova A M Malashenko I F Vyskubenko 《T?Sitologii?a i genetika》1984,18(2):123-128
A complex study of mutagenic activity of captan (a fungicide) in a series of standard test-systems has shown that the preparation induces gene mutations in certain Salmonella indicator strains (without metabolic activation), increases the frequency of mitotic crossing-over and gene conversion in yeasts (Saccharomyces), possesses a weak cytogenetic action on bone marrow cells in experimental animals, manifests no cytogenetic effect in the lymphocyte culture of human peripheral blood (including a system of microsomal activation). Genetic activity of captan cannot be a limiting criterion of its harmfulness. 相似文献
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Biophysics - Abstract—Two methods for studying the functioning of the mitochondrial ATP-sensitive potassium channel, which plays an important role in cell physiology and especially in cardio-... 相似文献
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N. Venediktova M. Shigaeva S. Belova K. Belosludtsev N. Belosludtseva O. Gorbacheva E. Lezhnev L. Lukyanova G. Mironova 《Molecular and cellular biochemistry》2013,383(1-2):261-269
The role of mitochondria in the inherited or ontogenetically acquired reactions of organism to stress is not studied enough. In the present work, we examined the functional state of the coupled respiratory chain, potassium and calcium transport and rate of hydrogen peroxide production on two rat lines: August and Wistar—which possess different resistance to emotional stress and hypoxia. It was established that the respiration rate and efficiency of oxidative phosphorylation were higher in August rats than in Wistar ones. In August rats, the rate of potassium transport and ATP-dependent mitochondrial swelling as well as the concentration of the ion in the mitochondrial matrix were almost twice as higher comparatively to those parameters in Wistar rats. The rate of H2O2 production was found to be decreased in the mitochondria of August rats. It was also demonstrated that the two rat lines differed by their resistance to the opening of the palmitate/Ca2+-induced pore and by their ability to retain calcium within mitochondria. The paper discusses the involvement of the mitochondrial ATP-dependent potassium channel in the adaptation of animals to adverse effects. 相似文献
4.
Streptomyces griseus 15 was subjected to the action of 1,4-bis-diazoacetyl butane (DAB) taken at concentrations of 10 to 50,000 micrograms/ml. Small doses (10-100 micrograms/ml) of DAB had no mutagenic action and activated the cultural growth (the viability and the survival rate of spores increased on solid media, while the biomass yield rised in liquid media). Experiments were conducted using the method of orthogonal planning of a bifactorial experiment, and the role of the exposure time rised with a decrease in the mutagen concentration. 相似文献
5.
The yeast mutants possessing enhanced sensitivity to detergents were obtained after treatment with ethyl methanesulfonate. The whole set of mutants may be divided into three groups, according to sensitivity to: cetylthreemethylammonium chloride detergents and dyes of the ethidium bromide type, detergents, dyes and antibiotics (gramycidin C and actinomycin D). The genetic analysis performed indicated that more than one gene are responsible for sensitivity. On the basis of the test for allelism mutants were distributed into three groups. It was shown that ethidium bromide is far more potent inducer of cytoplasmic petites in detergent-sensitive than in wild-type strains. 相似文献
6.
M Kh Shigaeva N B Akhmatullina N K Dzhangalina A S Zykova 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1984,(9):95-99
The effect of N-nitroso-N-methylurea (NMU), N-nitroso-N,N'-dimethylurea (NDMU) and N-nitroso-N-ethylurea (NEU) at doses less than 100 mkg/ml on mutability of Salmonella typhimurium strains of Ames' system (G-46, TA-1950, TA-1535, TA-100, TA-1538) has been studied. NMU and NEU at doses of 5-10 mkg/ml have been found to increase the survival and decrease the number of reversions from auxotrophity in histidine to prototrophity. The effect of given doses of NMU and NEU on bacteria repair activity has been shown. The role of pk M101 plasmide in this process is being discussed. NDMU in contrast to NMU and NEU induces read frome shift mutations and exhibits high mutagenous activity at all doses examined. 相似文献
7.
E. Yu. Talanov L. L. Pavlik M. I. Shigaeva N. V. Belosludtseva D. A. Moshkov G. D. Mironova 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2014,8(1):121-124
The electron microscopic study of thin sections of rat liver and heart using commercial specific antibodies against KIR.6.2 and secondary antibodies conjugated with colloidal gold was performed. It was found that the gold-labeled protein is localized in mitochondria of cardiomyocytes and hepatocytes but not in rough and smooth endoplasmic reticulum of hepatic cells and myofibrils of myocardium. In rat heart and liver mitochondria, the gold label was mainly located in mitochondrial cristae and was not found in mitochondrial matrix and intermembrane space. The data indicate that in heart and liver mitochondria there exists a protein similar in structure to the channel-forming subunit of a cytoplasmic potassium channel, KIR6.2. This is also supported by the presence of common modulators of cytoplasmic and mitochondrial ATP-dependent potassium channels. A possible role of the protein as a subunit of the mitochondrial ATP-dependent potassium channel is discussed. 相似文献
8.
Mironova GD Shigaeva MI Gritsenko EN Murzaeva SV Gorbacheva OS Germanova EL Lukyanova LD 《Journal of bioenergetics and biomembranes》2010,42(6):473-481
The mechanism of tissue protection from ischemic damage by activation of the mitochondrial ATP-dependent K+ channel (mitoKATP) remains unexplored. In this work, we have measured, using various approaches, the ATP-dependent mitochondrial K+ transport in rats that differed in their resistance to hypoxia. The transport was found to be faster in the hypoxia-resistant
rats as compared to that in the hypoxia-sensitive animals. Adaptation of animals to the intermittent normobaric hypoxia increased
the rate of transport. At the same time, the intramitochondrial concentration of K+ in the hypoxia-sensitive rats was higher than that in the resistant and adapted animals. This indicates that adaptation to
hypoxia stimulates not only the influx of potassium into mitochondria, but also K+/H+ exchange. When mitoKATP was blocked, the rate of the mitochondrial H2O2 production was found to be significantly higher in the hypoxia-resistant rats than that in the hypoxia-sensitive animals.
The natural flavonoid-containing adaptogen Extralife, which has an evident antihypoxic effect, increased the rate of the mitochondrial
ATP-dependent K+ transport in vitro and increased the in vivo tolerance of hypoxia-sensitive rats to acute hypoxia 5-fold. The involvement of the mitochondrial K+ transport in the mechanism of cell adaptation to hypoxia is discussed. 相似文献
9.
M. I. Shigaeva E. N. Gritsenko S. V. Murzaeva O. S. Gorbacheva E. Yu. Talanov G. D. Mironova 《Biophysics》2010,55(6):944-950
Changes in the rate of respiration and functioning of the ATP-dependent potassium channel in liver and heart mitochondria
of one-, three-, eight-, and 24-month-old Wistar male rats have been investigated. It was shown that the activity of the channel
in the mitochondria of both tissues in 24-month-old animals decreases more than three times, and the content of potassium,
1.5–2 times compared with young one-month-old rats. The changes occur against the background of age-related decrease of energy
supply in mitochondria, the respiratory complex-I undergoing the greatest changes upon aging. The decrease of channel activity
may be the result of changes in channel sensitivity to modulators and a decrease in the expression of mitochondrial K+-transporting
channel-protein with a molecular mass of 5.5 kDa upon aging found in this work. As a result, the functioning of not only the
mitoKATP but also the whole mitochondrial potassium cycle is impaired. 相似文献
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