排序方式: 共有37条查询结果,搜索用时 15 毫秒
1.
淀粉分支酶基因sbe是影响玉米直链淀粉含量的主要因素,淀粉分支酶分为3种即sbeI、sbeIIa和sbeIIb,其中sbeIIb对直链淀粉含量影响效应最大,抑制玉米淀粉分支酶sbeIIb基因的表达可减少支链淀粉的含量,从而达到提高直链淀粉的目的;ADP-葡萄糖焦磷酸化酶(AGPase)是直链淀粉合成的关键酶,通过提高AGP表达量同样可提高玉米直链淀粉含量。以此为目的分别克隆了sbeIIb一段375bp高度保守区,玉米SBE基因一段175bp内含子,AGP完整开放阅读框,大麦胚乳特异启动子和ADPG基因终止子。构建了sbeIIb基因正、反义的hpRNA发夹结构,将该发夹结构与上述基因分别连接到pCAM-BIA3301上;构建得到包含sbeIIb基因干扰结构与AGP基因过表达的pCAMB-RSA多基因胚乳特异表达载体。为此,pCAMB-RSA载体的成功构建将为高直链淀粉玉米的培育奠定基础。 相似文献
2.
Shuwan Zai Ruijie Han Wangen Zhao Chuang Ma Wenliang Huang Shengzhong Liu 《Liver Transplantation》2024,14(1):2303264
Defects within perovskite have been known to act as the nonradiative recombination centers, negatively impacting the carrier transport, which degrades the photovoltaic performance of perovskite solar cells (PSCs). Therefore, preparing a high-quality perovskite film is of vital significance. To this end, a room-temperature molten salt, dimethylamine formate (DMAFa), is introduced into perovskite precursor solution to regulate the crystallization process of CsPbI3 films. DMAFa can coordinate with Pb2+ as HCOO−-Pb2+ in the early stages, then HCOO−-Pb2+ is gradually displaced by I−-Pb2+ due to its decomposition during the subsequent annealing, thus delaying the crystallization rate, meanwhile, the DMA+ can interact with the uncoordinated Pb2+ to passivate defects of perovskite films, thereby, forming a high-quality CsPbI3 film with large grain size and low-defect density. As a result of this strategy, the power conversion efficiency is increased to 20.40%, and the open-circuit voltage is up to 1.21 V. These findings indicate that the introduction of DMAFa offers a fundamental way to achieve high-performance CsPbI3 PSCs. 相似文献
3.
4.
5.
6.
Lifang Sun Ying Wu Shengzhong Su Hongkui Liu Guang Yang Shipeng Li Xiaohui Shan Yaping Yuan 《Plant Cell, Tissue and Organ Culture》2012,109(2):271-286
Somatic embryogenesis is a complex developmental process that offers great potential for plant propagation. Although many
studies have shown that the generation of embryonic cells from somatic cells is accompanied by the synthesis of RNA and DNA
and by elevated enzymatic activity, the mechanism of the onset of somatic embryogenesis is not well understood. cDNA-amplified
fragment length polymorphism analysis was used to evaluate the gene expression pattern in embryogenic and non-embryogenic
of the inbred maize line H99 during the process of embryogenesis. We identified a total of 101 candidate genes associated
with the formation of maize embryonic calli. Based on the sequence analysis, these genes included 53 functionally-annotated
TDFs involved in such processes as energy production and conversion, cell division and signal transduction, suggesting that
somatic embryogenesis undergoes a complex process. Two full-length cDNA sequences, encoding KHCP (kinesin heavy chain like
protein) and TypA (tyrosine phosphorylation protein A), and partial sequences, encoding ARF-GEP (guanine nucleotide-exchange
protein of ADP ribosylation factor) homologs, were isolated from embryonic calli of maize and named ZmKHCP, ZmTypA and ZmARF-GEP,
respectively. Finally, the real-time qRT-PCR results showed that the expression levels of the three genes were significantly
higher in the embryonic calli than the non-embryonic calli. Thus, this study provides important clues to understanding the
induction of somatic embryogenesis in maize. The candidate genes associated with the formation of embryonic calli may offer
additional insights into the mechanism of somatic embryogenesis, and further research on the three candidate genes may determine
their role in increasing the rate of induction of embryonic calli, which may aid in the development of cultivars through transgenic
breeding. 相似文献
7.
8.
Jingru Zhang Dongliang Bai Zhiwen Jin Hui Bian Kang Wang Jie Sun Qian Wang Shengzhong Liu 《Liver Transplantation》2018,8(15)
All‐inorganic CsPbBrI2 perovskite has great advantages in terms of ambient phase stability and suitable band gap (1.91 eV) for photovoltaic applications. However, the typically used structure causes reduced device performance, primarily due to the large recombination at the interface between the perovskite, and the hole‐extraction layer (HEL). In this paper, an efficient CsPbBrI2 perovskite solar cell (PSC) with a dimensionally graded heterojunction is reported, in which the CsPbBrI2 material is distributed within bulk–nanosheet–quantum dots or 3D–2D–0D dimension‐profiled interface structure so that the energy alignment is optimized in between the valence and conduction bands of both CsPbBrI2 and the HEL layers. Specifically, the valence‐/conduction‐band edge is leveraged to bend with synergistic advantages: the graded combination enhances the hole extraction and conduction efficiency with effectively decreased recombination loss during the hole‐transfer process, leading to an enhanced built‐in electric field, hence a high VOC of as much as 1.19 V. The profiled structure induces continuously upshifted energy levels, resulting in a higher JSC of as much as 12.93 mA cm?2 and fill factor as high as 80.5%, and therefore record power conversion efficiency (PCE) of 12.39%. As far as it is known, this is the highest PCE for CsPbBrI2 perovskite‐based PSC. 相似文献
9.
Shengzhong Zhang Tao Wu Shijia Liu Xi Liu Ling Jiang Jianmin Wan 《Plant Molecular Biology Reporter》2016,34(4):748-760
10.