纹藤壶精子发生和成熟精子的超微结构

甲壳动物的精子形态各异.其中3个亚纲的精子具鞭毛,能运动,其它5个亚纲的精子无鞭毛且不运动.     

Selenious-β-lactoglobulin induces the apoptosis of human lung cancer A549 cells via an intrinsic mitochondrial pathway

In this study, the cytotoxic activity of selenious-β-lactoglobulin (Se-β-Lg) and the anticancer mechanism were investigated in human lung cancer A549 cells in vitro. MTT assay showed that Se-β-Lg at 200 μg/mL exhibited a significant suppression effect on A549 cells and the maximum inhibition rate reached 90% after 72 h treatment. Flow cytometry analysis revealed that 200 μg/mL of Se-β-Lg induced cell cycle arrest at G₀/G₁ phase. Cell apoptosis was induced via the generation of reactive oxygen species (ROS) and the decrease of mitochondrial membrane potential (ΔΨm) in a time-dependent manner. Furthermore, Se-β-Lg suppressed the expression of Bcl-2 and improved the level of Bax, leading to the release of cytochrome c and a higher expression of caspase-3 in A549 cells. In summary, Se-β-Lg could induce apoptosis in A549 cells via an intrinsic mitochondrial pathway and it might serve as a potential therapeutic agent for human lung cancer.     

东方扁虾精子的超微结构

利用电镜研究了东方扁虾（Ｔｈｅｎｕｓ ｏｒｉｅｎｔａｌｉｓ）精子的形态和结构。精子由核、膜复合物区和顶体区３部分组成。核内含非浓缩的染色质、微管及细纤维丝,外被核膜;５～６条辐射臂自核部位伸出,臂内充满微管。膜复合物区位于核与顶体之间,由许多膜片层结构及其衍生的囊泡共同组成。顶体区由顶体囊和围顶体物质组成,顶体结构复杂,由顶体帽、内顶体物质和外顶体物质等构成;围顶体物质呈细颗粒状,主要分布于顶体囊     

PLZF-RARα融合基因的生物学功能研究

构建了8个PLZF-RARα融合基因突变体.用“滞后”胶实验证实PLZF-RARα与PML-RARα一样,亦能以同二聚体的形式结合到维甲酸反应元件(RARE)上,且PLZF的POZ结构域介导PLZF-RARα同二聚体的形成和稳定.但两者与RARE的结合类型存在差异,在DR5G,PML-RARα的结合强度大于PLZF-RARα;而在DR5T,则是PLZF-RARα强于PML-RARα.进一步工作证实PLZF-RARα能与RXR形成异二聚体,并产生4种复合物.用免疫沉淀法发现PLZF-RARα亦能与PLZF形成异二聚体,而且也是通过POZ结构域介导PLZF-RARα和PLZF异二聚体的形成.同PML-RARα一样,PLZF-RARα对RARE的结合反应亦受维甲酸调控.     

中国沿海日本囊对虾线粒体16S rRNA基因序列变异及遗传分化

对中国东南沿海4个日本囊对虾地理群体16S rRNA基因片段进行了序列测定和分析.在获得的467bp 序列中,共检测到45个变异位点,多态位点比例为9.64%.具有10种单倍型,三亚群体单倍型多样性最高(0.900±0.161).各群体的核苷酸多样性(π)为0.0007～0.0082,其中三亚群体核苷酸多样性最为丰富,远高于湛江、北海和海口群体.群体间遗传分化指数(F_(st))为0.000～0.945.结合已报道的16S rRNA基因序列,构建了NJ分子系统树,中国海域日本囊对虾可分为3支.香港至台湾沿岸所有单倍型聚集为一分支,湛江、海口和北海群体内的所有单倍型聚集成另一分支,两分支分别属于已报道的变种Ⅰ和变种Ⅱ,但三亚群体内的4个单倍型单独聚集成一分支,与前两分支的核苷酸序列差异大(7.95%～8.35%),遗传分化明显,可能为一亲缘关系很远的新种(或变种).研究结果可为日本囊对虾的资源管理和遗传选育提供参考.     

Seasonal Variation in Diel Production Pattern of Centropages tenuiremis in Xiamen Waters, China

The seasonal variations in diel production pattern, and egg hatching time of the copepod Centropages tenuiremis in Xiamen waters, China, were investigated between January and June of 2003. The results show that in the winter–spring (January to early May) the adult females tended to lay subitaneous eggs at night, while in the beginning of summer (late May–June) they tended to lay diapause eggs as well as subitaneous eggs during the daytime. The egg hatching time negatively correlated with water temperature. These results, combined with the fact that C. tenuiremis migrates vertically in Xiamen waters, may explain the reproductive strategy of this dominant species in winter–spring.     

Seasonal reproductive biology of Centropages tenuiremis (copepoda) in Xiamen waters, People's Republic of China

The egg production rate (EPR) and the effects of environmentalvariations on diapause induction in the copepod Centropagestenuiremis in Xiamen waters were studied in 2002 and 2003. TheEPR ranged from 7.33 ± 8.14 (on 18 February 2003) to91.08 ± 20.61 (on 6 June 2003) eggs female^–1day^–1(mean ± 95% confidence limits), and displayed a significantseasonal pattern. Before the period of maximum egg production,EPR increased with seawater temperature and chlorophyll a concentration;but after the maximum, no significant relationship was foundbetween EPR and chlorophyll a concentration, and there was anegative linear relationship between EPR and temperature. Seawatertemperature and photoperiod, respectively, played a primaryrole in the switch to diapause egg production by the femalesas shown by single-factor probit analysis. However, the differencein effect between the two factors was demonstrated by binarylogistic multivariate analysis. These results suggest that thiscopepod rapidly increases subitaneous EPR by taking full advantageof conditions in favorable seasons (winter and spring). SubitaneousEPR decreases and there is a switch to the production of diapauseeggs that survive in the bottom sediments during unfavorableseasons (summer and autumn) in Xiamen waters.     

长毛对虾卵子皮层反应的研究

Morphology of the cortical reaction in the eggs of Penaeus penicillatus was studied with the light microscopy,scanning electron microscopy　and transmission electron microscopy. The cortical reaction is divided into four stages. These stages are unreacted stages, early stages, corona stages and dissipation stage. The cortical rods were released and formed a jelly coating around the surface of the egg. The jelly coating remained until the first cleavage had finished. In the end, the hatching membrane appeared around the egg. It is believed that these cortical reaction are responsible for the prevention of polyspermy by both a chemical and physical block and that also may establish a microenvironment inside a touch chorionic membrane for the developing embryo.     

银染技术在生殖细胞研究中的应用

新近对传统的银染技术作出改良,以氨银反应观察精子发生及受精过程中碱性蛋白的更替,以Ａｇ－Ａｓ反应观察精子发生过程中ＮＯＲ,嗜银细胞器,细胞骨架及其它嗜银成份的变化以及皮层皮应中嗜银成分的变化。     

PLZF基因大致结构的研究

报道了与急性早幼粒细胞白血病(APL)t(11;17)变异型易位有关的PLZF基因结构及表达.该基因至少长130kb,由7个外显子和6个内含子组成.发现外显子2中存在着替换剪接现象,且剪接供体信号为不典型的GA.PLZF基因的断裂点主要丛集于内含子3,该基因的表达具有组织特异性.