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Mala Ranghoo‐Sanmukhiya Joyce Govinden‐Soulange Christophe Lavergne Shannoo Khoyratty Denis Da Silva Michel Frederich Hippolyte Kodja 《Phytochemical analysis : PCA》2010,21(6):566-574
Introduction – Aloe tormentorii, A. purpurea and A. macra are used as multipurpose folk medicines in Réunion and Mauritius Islands and are mistaken for the introduced Aloe vera. Objective – To compare the phytochemical, antimicrobial and DNA profiles of Aloe endemic to Mauritius and Réunion with the profiles of A. vera. Methodology – Leaf extracts of these Aloe species were analysed using standard phytochemical screening techniques, TLC and by HPLC. These extracts were also assayed for antimicrobial activity using microdilution techniques. Genetic diversity was studied using RAPD markers. Results – Phytochemical and antimicrobial assays and RAPD analysis showed that Mascarene Aloe species were very different from A. vera. Conclusion – This study is the first report highlighting the differences between Aloe sp.p from Mascarene and Aloe vera at the metabolic and genomic level. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
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Daneshwar?PuchooaEmail author Sher-Ullah?Shahnoo?Sulliman?Khoyratty 《Plant Molecular Biology Reporter》2004,22(2):195-196
DNA extraction is difficult in many plants because of metabolites that interfere with DNA isolation procedures and subsequent
applications such as DNA restriction, amplification, and cloning. We developed the first reliable and efficient method for
isolatingVictoria amazonica genomic DNA that is free from polysaccharides and polyphenols. This protocol uses 1.5 M NaCl, 2% polyvinylpyrrolidone (PVP)
(Mr 1000), 5% mercaptoethanol, 0.12% sodium sulfite, and an incubation at 65°C for 4 h. The purity of isolated genomic DNA
was confirmed by means of high-performance liquid chromatography (HPLC) profile and spectrophotometric analyses (A260/230 ratio of 1.836, A260/280 of 1.842). DNA was obtained in the amount of 387 μg per gram of leaf material, and it proved amenable to restriction digestion. 相似文献
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