全文获取类型
收费全文 | 11605篇 |
免费 | 957篇 |
国内免费 | 1205篇 |
专业分类
13767篇 |
出版年
2024年 | 36篇 |
2023年 | 154篇 |
2022年 | 417篇 |
2021年 | 667篇 |
2020年 | 511篇 |
2019年 | 599篇 |
2018年 | 496篇 |
2017年 | 407篇 |
2016年 | 565篇 |
2015年 | 801篇 |
2014年 | 866篇 |
2013年 | 945篇 |
2012年 | 1135篇 |
2011年 | 988篇 |
2010年 | 569篇 |
2009年 | 557篇 |
2008年 | 609篇 |
2007年 | 482篇 |
2006年 | 447篇 |
2005年 | 366篇 |
2004年 | 320篇 |
2003年 | 273篇 |
2002年 | 251篇 |
2001年 | 230篇 |
2000年 | 172篇 |
1999年 | 144篇 |
1998年 | 96篇 |
1997年 | 94篇 |
1996年 | 94篇 |
1995年 | 73篇 |
1994年 | 65篇 |
1993年 | 46篇 |
1992年 | 62篇 |
1991年 | 31篇 |
1990年 | 39篇 |
1989年 | 29篇 |
1988年 | 26篇 |
1987年 | 21篇 |
1986年 | 16篇 |
1985年 | 23篇 |
1984年 | 10篇 |
1983年 | 10篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
1.
Food consumption, faecal production and nitrogen excretion by minnows, Phoxinus phoxinus , weighing 1–5.5 g were studied at five rations ranging from starvation to ad libitum and four temperatures ranging from 5 to 15°C.
The maximum rate of food consumption (Cmax ) was related to body weight ( W ) and temperature ( T ) by the relationship: C max = aWb1 Tb2 . There were significant daily variations in Cmax , which tended to decline over time. Absorption efficiency increased with increasing ration size and decreasing temperature. Body weight had no significant effect on the faecal production. The equation F = a Cb1 eb2 T described the relationship between faecal production ( F ), food consumption ( C ) and temperature. Ammonia-N predominated over urea-N in the excreta of most experimental fish. The proportion of urea-N in the total nitrogen excreted was generally higher at lower rations than at higher rations. Rates of nitrogen excretion increased with increased ration size and were, to a lesser extent, influenced by temperature. Body weight had no significant effect on the nitrogen excretion by feeding minnows. The equation N = a+bl T+b2 C described the effects of food consumption and temperature on nitrogen excretion ( N ) other than urea-N excretion. The relationship between urea-N excretion ( Nu ), food consumption and temperature was described by the equation Nu = aeb1T ((C+1) b 2.
On the average, 11 % of food energy was lost in faecal production and nitrogen excretion by minnows feeding on whiteworms, Enchytraeus spp. 相似文献
The maximum rate of food consumption (C
On the average, 11 % of food energy was lost in faecal production and nitrogen excretion by minnows feeding on whiteworms, Enchytraeus spp. 相似文献
2.
A determinant of polyomavirus virulence enhances virus growth in cells of renal origin. 总被引:3,自引:2,他引:1 下载免费PDF全文
J B Bolen S E Fisher K Chowdhury T C Shan J E Williams C J Dawe M A Israel 《Journal of virology》1985,53(1):335-339
We have identified a strain of polyomavirus, Py(L), which is unusual in causing acute morbidity and early death after inoculation of newborn mice. We determined that these animals died of kidney failure associated with extensive, virus-mediated destruction of renal tissue. Interestingly, the Py(L) strain infects baby mouse kidney cell cultures more efficiently than do other strains. 相似文献
3.
Preparation of [B23-D-alanine]des-(B25-B30)-hexapeptide-insulin by a combination of enzymic and non-enzymic synthesis. 下载免费PDF全文
Des-(B25-B30)-hexapeptide-insulin with B23-glycine replaced by D-alanine was prepared by a combination of enzymic and non-enzymic syntheses. The purified product was homogeneous in polyacrylamide-gel electrophoresis and could be crystallized. The biological activity in vivo of crystalline [B23-D-Ala]des-(B25-B30)-hexapeptide-insulin was determined as 58% of that of standard pig insulin (27 i.u./mg). 相似文献
4.
5.
兴安落叶松结实规律与长短枝习性的关系 总被引:1,自引:0,他引:1
1987年5月,大兴安岭林区发生的特大森林火灾,实属世界罕见,火灾面积达1.0×10~6ha 多。大量的火烧迹地亟待更新、无论是人工更新还是人工促进天然更新,其中关键的问题之一是种子的来源,在大兴安岭地区,兴 相似文献
6.
质粒YRP7用氯霉素法扩增,碱变性裂解法提取,酸酚法及核糖核酸酶纯化后,得到了高产量(5.6mg/L培养液),高纯度(A260:A280=2.0)的质粒制品,经转化实验及酶切分析确定YRP7具有下列特征:大小为5.41±0.10kb,可赋予宿主细胞AmP~r、Tet~r的表型,对大肠杆菌C600的转化频率为10~(-6)、转化效率为1.5×10~6转化子/mgDNA。限制性内切酶BamH Ⅰ、ECoRⅠ、Hind Ⅲ及PstⅠ在其分子上的切点数分别为1、2、2、2,并确定了各酶切片段的分子大小,对BanHⅠ的单切点,经插入失活法证实其位于Tet~r的基因上。由上述特征可确定,质粒YRP7是一个比较理想的克隆载体。 相似文献
7.
Yeast Mutants That Produce a Novel Type of Ascus Containing Asci Instead of Spores 总被引:2,自引:0,他引:2 下载免费PDF全文
Tetraploid yeast cells lacking BFR1 or overexpressing an essential gene BBP1 produce a novel type of ascus that contains asci instead of spores. We show here that the asci within an ascus likely arise because a/α spores undergo a second round of meiosis. Cells depleted of Bbp1p or lacking Bfr1p are defective in a number of processes such as nuclear segregation, bud formation, cytokinesis and nuclear spindle formation. Furthermore, deletion of BFR1 or overexpression of BBP1 leads to an increase in cell ploidy, indicating that Bfr1p and Bbp1p play roles in both the mitotic cell cycle and meiosis. Bfr1p and Bbp1p interact with each other in a two hybrid assay, further suggesting that they might form a complex important for cell cycle coordination. 相似文献
8.
大黄素对豚鼠结肠带平滑肌细胞电和收缩性能的影响 总被引:5,自引:1,他引:4
联合应用平滑肌肌力张力测量技术和细胞内微电极记录技术,同步地现测豚鼠结肠带平滑肌自发的肌源性电活动和力学活动,研究了大黄素的药物作用。大黄素能缩短膜电位的波动周期,从而缩短峰电位集簇发放的周期;相应地,可使平滑肌的分节律收缩加快,幅值指数升高。大黄素又能促使细胞膜电位自发的周期性波动的出现,导致峰电位的集簇发放;相应地,可使强直收缩转化为分节律收缩,即促进收缩形式向有利于肠道推进功能的方向转化。以上结果表明,大黄素能有效地提高豚鼠结肠带平滑肌细胞的电兴奋性和收缩功能,并且对其电学和力学活动的影响之间有明确的对应关系。 相似文献
9.
烟碱对乙酰胆碱诱发豚鼠回肠收缩作用的调节 总被引:3,自引:0,他引:3
在离休豚鼠回肠标本上,ACh诱发双相收缩反应:早期相收缩反应持续时间短,与神经节N受体有关;后期相收缩反应持续时间长,与平滑肌M受体有关。烟碱10μmol/L预孵育后,标本对烟碱激动神经节N受体诱发的收缩作用产生急性耐受,而ACh激动平滑肌M受体诱发的收缩作用增强。 相似文献
10.
Zhuang XC Sun YZ Cui J Zhu JM Jiang C Xiang QL Li CS 《Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology》1994,1(1):P61-P63
In the environment of microgravity, the disused atrophy of skeletal muscle, especially leg's muscle, would occur. The three purposes of this study were: 1. To observe the dynamic changes of disused atrophy of skeletal muscle under simulated weightlessness; 2. To approach the mechanism of disused atrophy of muscle; 3. To approach the countermeasures for reducing the degree of atrophy of muscle. 相似文献