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1.
2.
Chromosome-specific subsets of human alphoid DNA identified by a chromosome 2-derived clone 总被引:3,自引:0,他引:3
Mariano Rocchi Antonio Baldini Nicoletta Archidiacono Shabnam Lainwala Orlando J. Miller Dorothy A. Miller 《Genomics》1990,8(4):705-709
We have cloned an alphoid DNA fragment, pBS4D, from the DNA of a human-hamster hybrid cell line containing chromosome 2 as its only cytologically detectable human component. Under high stringency conditions, pBS4D hybridized in situ mostly to chromosome 2 and to a lesser extent to chromosomes 18 and 20. Restriction analysis using the DNA from selected somatic hybrid cell lines revealed that the genomic organization of this alphoid DNA differs on each of these three chromosomes. 相似文献
3.
Several reports have suggested that variations of albumin concentration in the incubation medium can modulate the magnitude of transferrin binding to the cells. We have investigated this problem further using K562 cells. In the absence of human serum albumin, transferrin binding demonstrated a non-saturable curve which, upon Scatchard analysis, showed two components with high and low affinities. In the presence of 0.5% human serum albumin, the low-affinity but not the high-affinity component was totally inhibited and, thus, the binding showed a saturation plateau at transferrin concentration of 6 micrograms/ml. Increasing concentrations of human serum albumin in the incubation medium led to progressive inhibition of transferrin binding, reaching a plateau at 0.2% human serum albumin. At this concentration transferrin binding was about 12 ng/10(6) cells, corresponding to the saturation plateau for high-affinity binding. Low-affinity transferrin binding in the absence of human serum albumin could readily be displaced by subsequent addition of albumin. Similar inhibition was obtained by another serum protein, ceruloplasmin, suggesting that this inhibition is not unique to albumin and may be a common property of all proteins. Incubation at 37 degrees C with 59Fe-labeled transferrin indicated that all iron uptake occurs through high-affinity binding. We conclude that the reported variations in magnitude of transferrin binding by the cell due to variations in albumin concentration are the result of inhibition of low-affinity binding of transferrin by albumin. 相似文献
4.
The role of liver endothelium in the binding and uptake of ceruloplasmin: studies with colloidal gold probe 总被引:6,自引:0,他引:6
To determine the mode of uptake of ceruloplasmin (CP) by liver, the protein was labeled with colloidal gold and infused into the portal vein. In cold almost all probes bound to the sinusoidal endothelium, and at 37 degrees C internalization via a system of coated pits and vesicles occurred. Only rarely did the probe appear to bypass the endothelium, moving to the abluminal side through the gaps between endothelial cells. In the endothelial cytoplasm, the probe was seen in coated vesicles, endosomes, tubules, and large vesicles which may have formed by fusion of endosomes and tubules. Moreover, externalization of the probe to the abluminal side was noted, and this also occurred via a system of coated vesicles. The findings suggest that the uptake of CP in the liver may be primarily a transendothelial phenomenon (transcytosis). 相似文献
5.
Summary Cellular and subcellular distribution of iron in the lamina propria of rat duodenum was studied after a single i.p. injection of iron dextran, using electron microscopy and peroxidase cytochemistry. X-ray spectrum microanalysis was used for positive identification of iron. Ironcontaining particles (IP) were found in the cytoplasm of three cell types, viz. macrophages, pericytic reticular cells and sheathing fibrocytes. IP-containing organelles in lamina propria cells were more heterogeneous compared to absorptive cells and, in addition, some differences were noted in the subcellular distribution of IP in the 3 cell types. A common denominator in these 3 cell types was the presence of endogenous peroxidase, also shared by Kupffer cells which are known to be involved in iron storage. Peroxidase activity was absent in absorptive epithelial cells. It is hypothesized that the cells of the lamina propria, like Kupffer cells, may be the site of storage of excess iron absorbed, releasing iron upon demand and migrating into the lumen to prevent iron overload. In this fashion they may regulate the exchange of iron with the environment. The presence of peroxidase in these as well as Kupffer cells, and its absence in absorptive cells also raises the possibility that this enzyme may be related to certain aspects of iron storing process. 相似文献
6.
The kinetics of the cellular uptake of iron-transferrin complex was studied in L1210 murine leukemia cells and rat reticulocytes using 125I-transferrin. Saturation of transferrin with iron was necessary for optimal uptake. Following the incubation of cells with the radiolabeled complex a biphasic pattern of uptake was observed. The initial phase was rapid and relatively temperature-independent and was not altered by ethylamine, an inhibitor of transglutaminase activity which is necessary for receptor-mediated endocytosis. This phase was considered to result from receptor-ligand interaction which could be reversed to a great degree by replacement with unlabeled transferrin. A plateau was then reached, indicating a saturation of receptors. After 30 min a second phase of uptake was indicated by the second rise in the curve. This phase was slow, relatively temperature-dependent and could be abolished by ethylamine. It was interpreted as evidence of internalization of the ligand. Analysis of the data from competition studies with unlabeled transferrin indicated that the first phase might itself comprise a reversible and an irreversible step with a ratio of 5 to 1.4 for bound transferrin. Thus, the cellular uptake of iron-transferrin complex may consist of a reversible ligand-receptor interaction. Conformational changes may render this interaction irreversible and the internalization of the ligand may then follow. 相似文献
7.
In addition to its many functions in biosynthesis, growth, coagulation and rheology, vascular endothelium is anatomically interposed between the vascular space and the tissue fluid. Recent evidence indicates that it mediates cellular and molecular exchange between these compartments. The exchange can occur through differentiated microdomains of endothelium such as fenestrae. These areas are differentiated with regard to surface charge, protein distribution within the lipid bilayer, membrane fluidity and other features. The exchange is also affected by certain characteristics of the molecule to be transported: molecular size, charge, shape and its carbohydrate content. Proportionately, the largest volume of exchange occurs across the endothelial cytoplasm by vesicular transport systems. Two systems are particularly in evidence; (a) receptor-mediated transcytosis which is specific, and (b) fluid-phase endocytosis. The molecule may become modified in transit and the modification may be of essence in determining its target point and its subsequent metabolism. While most of these modifications involve the carbohydrate moiety of the glycoproteins, glycosylation of non-glycoproteins such as albumin, may also be of physiological significance in transendothelial transport. By virtue of its transport potential, albumin can thus affect the transport of other substances. Recent advances in the molecular transport function of endothelium have been reviewed in the context of its physiological and clinical significance. The basis for the concept of a generalized tissue-blood barrier has been offered. 相似文献
8.
Variants of mouse leukaemia L1210 cells have been isolated in which cytotoxicity to dimethyl sulphate is not fully potentiated by ADP-ribosyl transferase inhibitor 3-aminobenzamide, as occurs in normal L1210 cells. These variants were selected after mutagenesis by growing the cells in dimethyl sulphate and 3-aminobenzamide. The characterisation of one of these variants is described. Variant 3 cells repair low doses of DNA damage in the presence of ADP-ribosyl transferase inhibitors. The Vmax of the ADP-ribosyl transferase enzyme in these cells is only increased 35% compared to normal wild-type L1210 cells. The basal DNA ligase I activity is increased 66% above wild-type whereas DNA ligase II activity appears to be unchanged. The most striking observation, however, is that the DNA ligase II activity is not increased after dimethyl sulphate treatment as occurs in wild-type L1210 cells. It seems that by increasing DNA ligase I levels these cells can survive DNA damage in the presence of 3-aminobenzamide. This variant (mutant) provides genetic evidence for our previously published hypothesis that (ADP-ribose)n biosynthesis is required for efficient DNA repair after DNA damage by monofunctional alkylating agents, because ADP-ribosyl transferase activity regulates DNA ligase activity. This variant is the first mammalian cell reported in which DNA ligase activity is altered, as far as we are aware. In yeast, a DNA ligase mutant has a cell division cycle (cdc) phenotype. Presumably, DNA ligase is essential for DNA synthesis, repair and recombination. The present variant provides further evidence that in mammalian cells, DNA ligase II activity is related to ADP-ribosyl transferase activity. 相似文献
9.
Nemati Sara Pazoki Hossein Mohammad Rahimi Hanieh Asadzadeh Aghdaei Hamid Shahrokh Shabnam Baghaei Kaveh Mirjalali Hamed Zali Mohammad Reza 《Molecular biology reports》2021,48(10):7041-7047
Molecular Biology Reports - Autophagy process is an important defense mechanism against intracellular infection. This process plays a critical role in limiting the development of Toxoplasma gondii.... 相似文献
10.
The system size dependence of the thermodynamic properties of electrolytic systems in "Spherical Boundary Conditions" (SBC) have been examined in this work. Coulombic systems were simulated with different system sizes (N ) for a wide range of concentrations, different ionic charges and solvent permittivities. The effects of system size upon the mean internal energy ?U? and mean ionic activity coefficient ?γ ±? values were determined. Our results indicated that there was no dependence of the thermodynamic properties upon system size in the non-Euclidean geometry. Different methods of extrapolating the thermodynamic properties to infinite numbers were studied in detail. In contrast to the Euclidean geometry, the method of extrapolating ?U? or ?γ ±? versus N -1, N -2/3 and N -1/3 was not statistically justifiable nor advantageous. Therefore, SBC is well suited to simulating systems involving long-range electrostatic interactions because the results do not dependent upon system size. 相似文献