Changes in the size of the nucleoli of the Purkinje cells in the canine cerebellum in the postresuscitation period

The changes in the size of Purkinje cell (PC) nucleolus in the lateral and medial cerebellum zones were studied in dogs with different degree of neurologic status recovery after clinical death of various etiology and duration. PC always possess one nucleolus in the control and experimental groups. In the case of complete neurologic status recovery of animals the area of PC nucleolus increases in both zones studied, irrespective of the cause of clinical death. In the case of neurologic disorders the increase in PC nucleolus area is clearly expressed only in the medial zone of the cerebellum, being insignificant in the lateral zone. It is suggested that adaptive characteristics of PC are distinct in the two compared zones, which leads to greater PC vulnerability in the lateral zone during deep hypoxia.     

Humoral immunity of persons inoculated with cultured antirabies vaccines according to abridged schedules

The production of virus-neutralizing antibodies in high titers has been found to occur in persons immunized with tissue-culture rabies vaccines, concentrated and non concentrated, intramuscularly in a dose of 1 ml in accordance with reduced immunization schedules.     

Hexameric purine nucleoside phosphorylase II from Escherichia coli K-12. Physico-chemical and catalytic properties and stabilization with substrates

Some properties of hexameric purine nucleoside phosphorylase II (EC 2.4.2.1) from Escherichia coli K-12 were studied. The enzyme obeys the Michaelis-Menten kinetics with respect to purine substrates (Km for inosine, deoxyinosine and hypoxanthine are equal to 492, 106 and 26.6 microM, respectively) and exhibits negative kinetic cooperativity towards phosphate and ribose-1-phosphate. The Hill coefficient is equal to approximately 0.5 for both substrates. Hexameric purine nucleoside phosphorylase II is not a metal-dependent enzyme; its activity is inhibited by Cu2+, Zn2+, Ni2+ and SO4(2-). The enzyme is the most stable at pH 6.0; it contains essential thiol groups. All substrates partly protect the enzyme against inactivation by 5.5'-dithiobis(2-nitrobenzoic acid) and heat-inactivation and, with the exception of phosphate-against inactivation by p-chloromercuribenzoate. Hypoxanthine, especially in combination with phosphate, afford the best protection against inactivation.     

Effects of vitamins A, E, C and P on intensity of blood coagulation in experimental animals]

In experiment on white rats it was shown that preventive 12-day administration of vitamins A, E, C, P decreases the death rate of animals with exogenous thromboplastinemia and reduces hemocoagulative changes, microcirculation disorders, destructive changes of functionally active elements of inner organs. The protective effect of the above vitamins combination in thromboplastinemia is promoted by hypoactivity of platelet aggregation, by low thromboplastic activity of erythrocytes, by limited destruction of vascular endothelium.     

Immunoenzyme determination of neurospecific antigen 10-40-4 in human and animal tissue and organ extracts

IEA of brain specific antigen 10-40-4 in human and animal organs and tissues was elaborated. The method permits measuring the concentration of the antigen within the range from 1 to 120 ng/ml. The use of the method made it possible to confirm brain specificity of protein 10-40-4, since its brain content is 1500 to 2000 times higher than in other organs, and to estimate the percentage of cross-reaction between antigenic determinants of brain specific antigen 10-40-4 from different species of animals.     

Cryofractographic study of intercellular junctions in the populations of agar-cultivated Bordetella pertussis

The characteristic feature of replicas obtained from the freeze-fractures of B. pertussis unfixed cultures developing on casein charcoal agar for 1-7 days is the associative growth of highly polymorphic cells, ensured by the ramified system of intercellular connections (IC) formed by the derivatives of the outer layers of the cell wall. This proves that the associative location of bacterial cells, linked by numerous IC, in the preparation is not the artefact appearing in the process of their chemical fixation. In replicas obtained from the freeze-fractures of B. pertussis cultures, previously fixed with glutaraldehyde, osmic acid and uranyl acetate, oval cells with the cytoplasm having a relatively homogeneous structure and with the smoothed-out three-layer cell wall prevail. As a rule, IC are limited to the sites of direct contacts between individual cells.     

Stable paper indicator systems for rapid identification of microorganisms]

The authors prepared under experimental-industrial conditions paper indicator systems for the express identification of microbes, including carbohydrate discs (by the method of Nikitin et al.), and newly worked out types for the determination of the activity of cytochromoxidase, urease, indol formation, and indicator amino acid decarboxylases (lysin, ornithin, arginine). The use of paper indicator discs proved to be expedient for rationalization of laboratory diagnosis of bacterial intestinal infections.     

Relation between the radioprotective effect of helium-neon laser radiation on bacterial cells and the time period between 2 types of irradiation

The effect of helium-neon laser radiation (lambda = 633 nm) given to E. coli K-12 cells of various genotypes 4 h following X- or alpha-irradiation was shown to increase the number of viable cells. The irreversible ingradient of a radiation injury to cells remained invariable during the first 60 min after irradiation its values being minimal.     

Neurospecific and neuron-specific enolase levels in human nerve tissue at early stages of embryogenesis

Neurospecific and neuro-nonspecific isozymes of glycolytic enzyme enolase known to be molecular markers of neuronal and glial cells respectively were isolated from human brain. Using immunoenzyme assay, the content of these proteins was measured in the brain of 7-13-week human embryos and adult subjects. The content of both isoenzymes gradually increases attaining constant level in 10-13-week embryos. Relative content of neuro-nonspecific enolase in the brain of 10-13-week embryos is 1 1/2 times higher than that in adults, whereas the content of neurospecific enolase amounts only to 2% of its level in the adult brain.